Electro-autotrophic synthesis of higher alcohols

Liao, James C.; Cho, Kwang Myung

Title: Electro-autotrophic synthesis of higher alcohols

Patent · Tue Oct 06 00:00:00 EDT 2015

OSTI ID:1222639

Liao, James C.; Cho, Kwang Myung

The disclosure provides a process that converts CO.sub.2 to higher alcohols (e.g. isobutanol) using electricity as the energy source. This process stores electricity (e.g. from solar energy, nuclear energy, and the like) in liquid fuels that can be used as high octane number gasoline substitutes. Instead of deriving reducing power from photosynthesis, this process derives reducing power from electrically generated mediators, either H.sub.2 or formate. H.sub.2 can be derived from electrolysis of water. Formate can be generated by electrochemical reduction of CO.sub.2. After delivering the reducing power in the cell, formate becomes CO.sub.2 and recycles back. Therefore, the biological CO.sub.2 fixation process can occur in the dark.

View Patent

Cite

Export

Save

Research Organization:: Univ. of California, Oakland, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AR0000085

Assignee:: The Regents of the University of California (Oakland, CA)

Patent Number(s):: 9,150,889

Application Number:: 13/522,288

OSTI ID:: 1222639

Resource Relation:: Patent File Date: 2011 Jan 15

Country of Publication:: United States

Language:: English

References (60)

Microbial hybrid promoters Deboer, Herman A. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4551433 US Patent Document 4,551,433	patent	November 1985
Process for amplifying nucleic acid sequences Mullis, Kary B. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4683202 US Patent Document 4,683,202	patent	July 1987
Direct molecular cloning of primer extended DNA containing an alkane diol Wallace, Robert Bruce; Witney, Franklin R. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5426039 US Patent Document 5,426,039	patent	June 1995
Synthetic insecticidal crystal protein gene having a modified frequency of codon usage Adang, Michael J.; Murray, Elizabeth E. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6015891 US Patent Document 6,015,891	patent	January 2000
Biofuel Production Yoshikuni, Yasuo; Kashiyama, Yuki https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20090139134 US Patent Application 12/245537; 20090139134	patent-application	June 2009
Engineered microorganisms for producing N-butanol and related methods Buelter, Thomas; Hawkins, Andrew C.; Kersh, Kalib https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20090155869 US Patent Application 11/949724; 20090155869	patent-application	June 2009
Polymerase Chain Reaction Arnheim, Norman; Levenson, Corey H. Chemical & Engineering News, Vol. 68, Issue 40 https://doi.org/10.1021/cen-v068n040.p036	journal	October 1990
Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde Atsumi, Shota; Higashide, Wendy; Liao, James C. Nature Biotechnology, Vol. 27, Issue 12, p. 1177-1180 https://doi.org/10.1038/nbt.1586	journal	November 2009
Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants the Keio collection Baba, Tomoya; Ara, Takeshi; Hasegawa, Miki Molecular Systems Biology, Vol. 2, Article No. 2006.0008 https://doi.org/10.1038/msb4100050	journal	February 2006
Blunt-end and single-strand ligations by Escherichia coli ligase: influence on an in vitro amplication scheme Barringer, Kevin J.; Orgel, Leslie; Wahl, Geoff Gene, Vol. 89, Issue 1, p. 117-122 https://doi.org/10.1016/0378-1119(90)90213-B	journal	April 1990
Sequences and characterization of hupU and hupV genes of Bradyrhizobium japonicum encoding a possible nickel-sensing complex involved in hydrogenase expression. Black, L. K.; Fu, C.; Maier, R. J. Journal of Bacteriology, Vol. 176, Issue 22 https://doi.org/10.1128/jb.176.22.7102-7106.1994	journal	November 1994
REDOX REGULATION: A Broadening Horizon Buchanan, Bob B.; Balmer, Yves Annual Review of Plant Biology, Vol. 56, Issue 1 https://doi.org/10.1146/annurev.arplant.56.032604.144246	journal	June 2005
Oxygen Tolerance of the H ₂ -sensing [NiFe] Hydrogenase from Ralstonia eutropha H16 Is Based on Limited Access of Oxygen to the Active Site Buhrke, Thorsten; Lenz, Oliver; Krauss, Norbert Journal of Biological Chemistry, Vol. 280, Issue 25 https://doi.org/10.1074/jbc.M503260200	journal	April 2005
Reduction of Unusual Iron-Sulfur Clusters in the H ₂ -sensing Regulatory Ni-Fe Hydrogenase from Ralstonia eutropha H16 Buhrke, Thorsten; Löscher, Simone; Lenz, Oliver Journal of Biological Chemistry, Vol. 280, Issue 20 https://doi.org/10.1074/jbc.M500601200	journal	March 2005
Oxidizing enzymes as biocatalysts Burton, Stephanie G. Trends in Biotechnology, Vol. 21, Issue 12, p. 543-549 https://doi.org/10.1016/j.tibtech.2003.10.006	journal	December 2003
Long PCR Cheng, Suzanne; Chang, Sheng-Yung; Gravitt, Patti Nature, Vol. 369, Issue 6482 https://doi.org/10.1038/369684a0	journal	June 1994
Genomic View of Energy Metabolism in <i>Ralstonia eutropha</i> H16 Cramm, Rainer Journal of Molecular Microbiology and Biotechnology, Vol. 16, Issue 1-2 https://doi.org/10.1159/000142893	journal	January 2009
TransTerm: a database of translational signals Dalphin, M. Nucleic Acids Research, Vol. 24, Issue 1 https://doi.org/10.1093/nar/24.1.216	journal	January 1996
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products Datsenko, K. A.; Wanner, B. L. Proceedings of the National Academy of Sciences, Vol. 97, Issue 12, p. 6640-6645 https://doi.org/10.1073/pnas.120163297	journal	May 2000
The hupTUV operon is involved in negative control of hydrogenase synthesis in Rhodobacter capsulatus. Elsen, S.; Colbeau, A.; Chabert, J. Journal of Bacteriology, Vol. 178, Issue 17 https://doi.org/10.1128/jb.178.17.5174-5181.1996	journal	September 1996
Light-responsive gene expression in cyanobacteria. Golden, S. S. Journal of Bacteriology, Vol. 177, Issue 7 https://doi.org/10.1128/jb.177.7.1651-1654.1995	journal	April 1995
Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication. Guatelli, J. C.; Whitfield, K. M.; Kwoh, D. Y. Proceedings of the National Academy of Sciences, Vol. 87, Issue 5, p. 1874-1878 https://doi.org/10.1073/pnas.87.5.1874	journal	March 1990
Dehydrogenases for the synthesis of chiral compounds Hummel, Werner; Kula, Maria-Regina European Journal of Biochemistry, Vol. 184, Issue 1 https://doi.org/10.1111/j.1432-1033.1989.tb14983.x	journal	September 1989
Expression of a Gene Cluster kaiABC as a Circadian Feedback Process in Cyanobacteria Ishiura, M. Science, Vol. 281, Issue 5382 https://doi.org/10.1126/science.281.5382.1519	journal	September 1998
Microbial production of poly- D- 3-hydroxybutyrate from CO 2 A., Ishizaki; K., Tanaka; N., Taga Applied Microbiology and Biotechnology, Vol. 57, Issue 1-2 https://doi.org/10.1007/s002530100775	journal	October 2001
Cyanobacterial daily life with Kai-based circadian and diurnal genome-wide transcriptional control in Synechococcus elongatus Ito, H.; Mutsuda, M.; Murayama, Y. Proceedings of the National Academy of Sciences, Vol. 106, Issue 33, p. 14168-14173 https://doi.org/10.1073/pnas.0902587106	journal	July 2009
Quinone sensing by the circadian input kinase of the cyanobacterial circadian clock Ivleva, N. B.; Gao, T.; LiWang, A. C. Proceedings of the National Academy of Sciences, Vol. 103, Issue 46, p. 17468-17473 https://doi.org/10.1073/pnas.0606639103	journal	November 2006
Structural Insights into a Circadian Oscillator Johnson, C. H.; Egli, M.; Stewart, P. L. Science, Vol. 322, Issue 5902 https://doi.org/10.1126/science.1150451	journal	October 2008
The H2 Sensor of Ralstonia eutropha Is a Member of the Subclass of Regulatory [NiFe] Hydrogenases Kleihues, L.; Lenz, O.; Bernhard, M. Journal of Bacteriology, Vol. 182, Issue 10 https://doi.org/10.1128/JB.182.10.2716-2724.2000	journal	May 2000
The hydrogenases of Thiocapsa roseopersicina Kovács, K. L.; Kovács, Á. T.; Maróti, G. Biochemical Society Transactions, Vol. 33, Issue 1 https://doi.org/10.1042/BST0330061	journal	February 2005
Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format. Kwoh, D. Y.; Davis, G. R.; Whitfield, K. M. Proceedings of the National Academy of Sciences, Vol. 86, Issue 4, p. 1173-1177 https://doi.org/10.1073/pnas.86.4.1173	journal	February 1989
A ligase-mediated gene detection technique Landegren, U.; Kaiser, R.; Sanders, J. Science, Vol. 241, Issue 4869 https://doi.org/10.1126/science.3413476	journal	August 1988
Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris Larimer, Frank W; Chain, Patrick; Hauser, Loren Nature Biotechnology, Vol. 22, Issue 1, p. 55-61 https://doi.org/10.1038/nbt923	journal	December 2003
A novel multicomponent regulatory system mediates H2 sensing in Alcaligenes eutrophus Lenz, O.; Friedrich, B. Proceedings of the National Academy of Sciences, Vol. 95, Issue 21 https://doi.org/10.1073/pnas.95.21.12474	journal	October 1998
Thioredoxin-linked processes in cyanobacteria are as numerous as in chloroplasts, but targets are different Lindahl, M.; Florencio, F. J. Proceedings of the National Academy of Sciences, Vol. 100, Issue 26, p. 16107-16112 https://doi.org/10.1073/pnas.2534397100	journal	December 2003
Circadian orchestration of gene expression in cyanobacteria. Liu, Y.; Tsinoremas, N. F.; Johnson, C. H. Genes & Development, Vol. 9, Issue 12 https://doi.org/10.1101/gad.9.12.1469	journal	June 1995
Dual Role of Cysteine 172 in Redox Regulation of Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase Activity and Degradation Marcus, Y.; Altman-Gueta, H.; Finkler, A. Journal of Bacteriology, Vol. 185, Issue 5 https://doi.org/10.1128/JB.185.5.1509-1517.2003	journal	March 2003
Overexpression of a cyanobacterial fructose-1,6-/sedoheptulose-1, 7-bisphosphatase in tobacco enhances photosynthesis and growth Miyagawa, Yoshiko; Tamoi, Masahiro; Shigeoka, Shigeru Nature Biotechnology, Vol. 19, Issue 10 https://doi.org/10.1038/nbt1001-965	journal	October 2001
Codon usage in plant genes Murray, Elizabeth E.; Lotzer, Jeff; Eberle, Mary Nucleic Acids Research, Vol. 17, Issue 2 https://doi.org/10.1093/nar/17.2.477	journal	January 1989
Global gene repression by KaiC as a master process of prokaryotic circadian system Nakahira, Y.; Katayama, M.; Miyashita, H. Proceedings of the National Academy of Sciences, Vol. 101, Issue 3, p. 881-885 https://doi.org/10.1073/pnas.0307411100	journal	January 2004
Improvement for L-leucine production in a continuously operated enzyme membrane reactor Ohshima, Toshihisa; Wandrey, Christian; Kula, Maria-Regina Biotechnology and Bioengineering, Vol. 27, Issue 11, p. 1616-1618 https://doi.org/10.1002/bit.260271118	journal	November 1985
The multisubunit chloroplast RNA polymerase A from mustard (Sinapis alba L.): Integration of a prokaryotic core into a larger complex with organelle-specific functions Pfannschmidt, Thomas; Ogrzewalla, Karsten; Baginsky, Sacha European Journal of Biochemistry, Vol. 267, Issue 1 https://doi.org/10.1046/j.1432-1327.2000.00991.x	journal	January 2000
Genome sequence of the bioplastic-producing “Knallgas” bacterium Ralstonia eutropha H16 Pohlmann, Anne; Fricke, Wolfgang Florian; Reinecke, Frank Nature Biotechnology, Vol. 24, Issue 10 https://doi.org/10.1038/nbt1244	journal	September 2006
Regulation of Uptake Hydrogenase and Effects of Hydrogen Utilization on Gene Expression in Rhodopseudomonas palustris Rey, F. E.; Oda, Y.; Harwood, C. S. Journal of Bacteriology, Vol. 188, Issue 17 https://doi.org/10.1128/JB.00381-06	journal	August 2006
Phosphotransfer Reactions of the CbbRRS Three-Protein Two- Component System from Rhodopseudomonas palustris CGA010 Appear To Be Controlled by an Internal Molecular Switch on the Sensor Kinase Romagnoli, S.; Tabita, F. R. Journal of Bacteriology, Vol. 189, Issue 2 https://doi.org/10.1128/JB.01326-06	journal	October 2006
The membrane-bound hydrogenase of Alcaligenes eutrophus. I. Solubilization, purification, and biochemical properties Schink, Bernhard; Schlegel, Hans G. Biochimica et Biophysica Acta (BBA) - Enzymology, Vol. 567, Issue 2, p. 315-324 https://doi.org/10.1016/0005-2744(79)90117-7	journal	April 1979
Purification and properties of soluble hydrogenase from Alcaligenes eutrophus H 16 Schneider, Klaus; Schlegel, Hans G. Biochimica et Biophysica Acta (BBA) - Enzymology, Vol. 452, Issue 1, p. 66-80 https://doi.org/10.1016/0005-2744(76)90058-9	journal	November 1976
Air Pollution and Climate-Forcing Impacts of a Global Hydrogen Economy Schultz, M. G. Science, Vol. 302, Issue 5645 https://doi.org/10.1126/science.1089527	journal	October 2003
Hydrogen metabolism and nitrogen fixation in wild type and Nif⁻ mutants of Rhodopseudomonas acidophila Siefert, Eike; Pfennig, Norbert Biochimie, Vol. 60, Issue 3, p. 261-265 https://doi.org/10.1016/S0300-9084(78)80822-0	journal	June 1978
Stabilization of NAD-dependent formate dehydrogenase from Candida boidinii by site-directed mutagenesis of cysteine residues: Site-directed mutagenesis of formate dehydrogenase. Slusarczyk, Heike; Felber, Stephan; Kula, Maria-Regina European Journal of Biochemistry, Vol. 267, Issue 5 https://doi.org/10.1046/j.1432-1327.2000.01123.x	journal	March 2000
Circadian rhythms in gene transcription imparted by chromosome compaction in the cyanobacterium Synechococcus elongatus Smith, R. M.; Williams, S. B. Proceedings of the National Academy of Sciences, Vol. 103, Issue 22, p. 8564-8569 https://doi.org/10.1073/pnas.0508696103	journal	May 2006
Nasba Sooknanan, Roy; Malek, Lawrence T. Nature Biotechnology, Vol. 13, Issue 6 https://doi.org/10.1038/nbt0695-563	journal	June 1995
Acquisition of a new type of fructose-1,6-bisphosphatase with resistance to hydrogen peroxide in cyanobacteria: molecular characterization of the enzyme from Synechocystis PCC 6803 Tamoi, Masahiro; Murakami, Akiko; Takeda, Toru Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 1383, Issue 2 https://doi.org/10.1016/S0167-4838(97)00208-2	journal	April 1998
The Calvin cycle in cyanobacteria is regulated by CP12 via the NAD(H)/NADP(H) ratio under light/dark conditions: Regulation of the Calvin cycle by cyanobacterial CP12 Tamoi, Masahiro; Miyazaki, Takashi; Fukamizo, Tamo The Plant Journal, Vol. 42, Issue 4 https://doi.org/10.1111/j.1365-313X.2005.02391.x	journal	April 2005
Molecular Characterization and Resistance to Hydrogen Peroxide of Two Fructose-1,6-bisphosphatases fromSynechococcusPCC 7942 Tamoi, Masahiro; Ishikawa, Takahiro; Takeda, Toru Archives of Biochemistry and Biophysics, Vol. 334, Issue 1 https://doi.org/10.1006/abbi.1996.0425	journal	October 1996
Amplifying Genes: PCR and Its Alternatives Van Brunt, Jennifer Nature Biotechnology, Vol. 8, Issue 4 https://doi.org/10.1038/nbt0490-291	journal	April 1990
Evolutionary conserved light regulation of Calvin cycle activity by NADPH-mediated reversible phosphoribulokinase/CP12/ glyceraldehyde-3-phosphate dehydrogenase complex dissociation Wedel, Norbert; Soll, Jürgen Proceedings of the National Academy of Sciences, Vol. 95, Issue 16 https://doi.org/10.1073/pnas.95.16.9699	journal	August 1998
Cofactor Regeneration at the Lab Scale Wichmann, R.; Vasic-Racki, D. Technology Transfer in Biotechnology https://doi.org/10.1007/b98911	book	January 2005
The ligation amplification reaction (LAR)—Amplification of specific DNA sequences using sequential rounds of template-dependent ligation Wu, Dan Y.; Wallace, R. Bruce Genomics, Vol. 4, Issue 4, p. 560-569 https://doi.org/10.1016/0888-7543(89)90280-2	journal	May 1989
Biosynthesis and Compositional Regulation of Poly[(3-hydroxybutyrate)-co-(3-hydroxyhexanoate)] in RecombinantRalstonia eutropha Expressing Mutated Polyhydroxyalkanoate Synthase Genes Tsuge, Takeharu; Saito, Yu; Kikkawa, Yoshihiro Macromolecular Bioscience, Vol. 4, Issue 3 https://doi.org/10.1002/mabi.200300077	journal	March 2004

Cited By (1)

Electro-autotrophic synthesis of higher alcohols Liao, James C.; Cho, Kwang Myung https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9150889 US Patent Document 9,150,889	patent	October 2015

Similar Records

Electro-autotrophic synthesis of higher alcohols

Patent · Tue Nov 01 00:00:00 EDT 2016 · OSTI ID:1222639

Liao, James C.; Cho, Kwang Myung

Assessment of ether and alcohol fuels from coal. Volume I. Summary. Final report. [Mixtures of ethers and higher alcohols with gasoline]

Technical Report · Tue Mar 01 00:00:00 EST 1983 · OSTI ID:1222639

Liquid Fuel From Renewable Electricity and Bacteria: Electro-Autotrophic Synthesis of Higher Alcohols

Program Document · Thu Jul 01 00:00:00 EDT 2010 · OSTI ID:1222639

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Title: Electro-autotrophic synthesis of higher alcohols

Citation Formats

References (60)

Cited By (1)

Similar Records

Related Subjects