Goniometer-based femtosecond crystallography with X-ray free electron lasers

Cohen, Aina E.; Soltis, S. Michael; González, Ana; Aguila, Laura; Alonso-Mori, Roberto; Barnes, Christopher O.; Baxter, Elizabeth L.; Brehmer, Winnie; Brewster, Aaron S.; Brunger, Axel T.; Calero, Guillermo; Chang, Joseph F.; Chollet, Matthieu; Ehrensberger, Paul; Eriksson, Thomas L.; Feng, Yiping; Hattne, Johan; Hedman, Britt; Hollenbeck, Michael; Holton, James M.; Keable, Stephen; Kobilka, Brian K.; Kovaleva, Elena G.; Kruse, Andrew C.; Lemke, Henrik T.; Lin, Guowu; Lyubimov, Artem Y.; Manglik, Aashish; Mathews, Irimpan I.; McPhillips, Scott E.; Nelson, Silke; Peters, John W.; Sauter, Nicholas K.; Smith, Clyde A.; Song, Jinhu; Stevenson, Hilary P.; Tsai, Yingssu; Uervirojnangkoorn, Monarin; Vinetsky, Vladimir; Wakatsuki, Soichi; Weis, William I.; Zadvornyy, Oleg A.; Zeldin, Oliver B.; Zhu, Diling; Hodgson, Keith O.

doi:10.1073/pnas.1418733111

Title: Goniometer-based femtosecond crystallography with X-ray free electron lasers

Journal Article · Fri Oct 31 00:00:00 EDT 2014 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1418733111· OSTI ID:1221828

Cohen, Aina E. ^[1]; Soltis, S. Michael ^[1]; González, Ana ^[1]; Aguila, Laura ^[1]; Alonso-Mori, Roberto ^[1]; Barnes, Christopher O. ^[2]; Baxter, Elizabeth L. ^[3]; Brehmer, Winnie ^[1]; Brewster, Aaron S. ^[4]; Brunger, Axel T. ^[5]; Calero, Guillermo ^[2]; Chang, Joseph F. ^[1]; Chollet, Matthieu ^[1]; Ehrensberger, Paul ^[1]; Eriksson, Thomas L. ^[1]; Feng, Yiping ^[1]; Hattne, Johan ^[4]; Hedman, Britt ^[1]; Hollenbeck, Michael ^[1]; Holton, James M. ^[6] more »

SLAC National Accelerator Lab., Menlo Park, CA (United States)
Univ. of Pittsburgh, PA (United States)
SLAC National Accelerator Lab., Menlo Park, CA (United States); Stanford Univ., CA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Stanford Univ., CA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, San Francisco, CA (United States)
Montana State Univ., Bozeman, MT (United States)

The emerging method of femtosecond crystallography (FX) may extend the diffraction resolution accessible from small radiation-sensitive crystals and provides a means to determine catalytically accurate structures of acutely radiation-sensitive metalloenzymes. Automated goniometer-based instrumentation developed for use at the Linac Coherent Light Source enabled efficient and flexible FX experiments to be performed on a variety of sample types. In the case of rod-shaped Cpl hydrogenase crystals, only five crystals and about 30 min of beam time were used to obtain the 125 still diffraction patterns used to produce a 1.6-Å resolution electron density map. With smaller crystals, high-density grids were used to increase sample throughput; 930 myoglobin crystals mounted at random orientation inside 32 grids were exposed, demonstrating the utility of this approach. Screening results from cryocooled crystals of β₂-adrenoreceptor and an RNA polymerase II complex indicate the potential to extend the diffraction resolution obtainable from very radiation-sensitive samples beyond that possible with undulator-based synchrotron sources.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-05CH11231; P41GM103393; GM102520; GM095887; GM073210; GM082250; GM094625; HHMI Collaborative Innovation Award (HCIA)

OSTI ID:: 1221828

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 48; ISSN 0027-8424

Publisher:: National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 101 works

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