The effects of cadmium of the growth and metallothionein expression of the bivalve larvae, crassostrea virginica
- Univ. of Charleston, WV (United States)
Oyster larvae, Crassostrea virginica, were exposed to 20 ppb of cadmium (Cd) and fed (mixture of Isochrysis galbana & Chaetoceros gracilis, 40mL) in the laboratory for 10 days. On the 0, 4, 7 and 10 day the larvae samples were taken and frozen. Then they were homogenized, centrifuged, ultrafiltered through a membrane separation technique used to segregate substances according to the molecular weight and size. The cytosolic protein was first partially purified by gel permeation, then by PAGE (Polyacrylamide Gel Electrophoresis). The controls and metal exposed larvae were evaluated on total wet weight and the metallothioneins (MT) were identified from the preparations using silver staining techniques. No significant changes could be detected in the controls. However, there was a great number dead at the beginning of the experiment. Cd accumulation began at the time of exposure. This suggests that surface area may play a role in determining short-term accumulation rates. Cd effects on growth (wet weight) was slightly different, the exposed weighed less than or equal to the controls. In addition, the Cd uptake via food played an insignificant role compared to direct uptake from sea water. Between day 0 and 7 there was a number of mortalities for the controls and exposed. In addition, there was a major weight change with the exposed, they appeared to weigh less than the controls on day 7, whereas on day 4 they weighed more. So weight is a very sensitive indicator of toxic stress.
- Research Organization:
- Medical Univ. of South Carolina, Charleston, SC (United States)
- OSTI ID:
- 121302
- Report Number(s):
- DOE/EW/50625-T25; ON: DE95016555; TRN: 95:007740-0003
- Resource Relation:
- Other Information: PBD: 1994; Related Information: Is Part Of Summer Undergraduate Research Program: Environmental studies; McMillan, J. [ed.]; PB: 127 p.
- Country of Publication:
- United States
- Language:
- English
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