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Title: Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging

Journal Article · · Structural Dynamics
DOI:https://doi.org/10.1063/1.4919641· OSTI ID:1212299
 [1];  [2];  [1];  [3];  [4];  [5];  [5];  [6]
  1. La Trobe Univ., Melbourne (Australia); CSIRO Manufacturing Flagship, Parkville (Australia)
  2. La Trobe Univ., Melbourne (Australia)
  3. Univ. of Oxford, Oxford (United Kingdom)
  4. SLAC National Accelerator Lab., Menlo Park, CA (United States); Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
  5. Argonne National Lab. (ANL), Argonne, IL (United States)
  6. La Trobe Univ., Melbourne (Australia); Melbourne Centre for Nanofabrication, Melbourne (Australia)
+ Show Author Affiliations

For laboratory and synchrotron based X-ray sources, radiation damage has posed a significant barrier to obtaining high-resolution structural data from biological macromolecules. The problem is particularly acute for micron-sized crystals where the weaker signal often necessitates the use of higher intensity beams to obtain the relevant data. Here, we employ a combination of techniques, including Bragg coherent diffractive imaging to characterise the radiation induced damage in a micron-sized protein crystal over time. The approach we adopt here could help screen for potential protein crystal candidates for measurement at X-ray free election laser sources.

Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
Grant/Contract Number:
AC02-06CH11357
OSTI ID:
1212299
Alternate ID(s):
OSTI ID: 1421048
Journal Information:
Structural Dynamics, Vol. 2, Issue 4; ISSN 2329-7778
Publisher:
American Crystallographic Association/AIPCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 12 works
Citation information provided by
Web of Science

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Cited By (3)

Resolution and dose dependence of radiation damage in biomolecular systems journal July 2019
Bragg coherent diffraction imaging and metrics for radiation damage in protein micro-crystallography text January 2017
Nano-scale imaging of the full strain tensor of specific dislocations extracted from a bulk sample text January 2019

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Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
proteins
radiation damage
x-ray diffraction
crystal structure
reciprocal space