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Title: Supplemental macronutrients and microbial fermentation products improve the uptake and transport of foliar applied zinc in sunflower (Helianthus annuus L.) plants. Studies utilizing micro X-ray florescence

Abstract

Enhancing nutrient uptake and the subsequent elemental transport from the sites of application to sites of utilization is of great importance to the science and practical field application of foliar fertilizers. The aim of this study was to investigate the mobility of various foliar applied zinc (Zn) formulations in sunflower (Helianthus annuus L.) and to evaluate the effects of the addition of an organic biostimulant on phloem loading and elemental mobility. This was achieved by application of foliar formulations to the blade of sunflower (H. annuus L.) and high-resolution elemental imaging with micro X-ray fluorescence (μ-XRF) to visualize Zn within the vascular system of the leaf petiole. Although no significant increase of total Zn in petioles was determined by inductively-coupled plasma mass-spectrometer, μ-XRF elemental imaging showed a clear enrichment of Zn in the vascular tissues within the sunflower petioles treated with foliar fertilizers containing Zn. The concentration of Zn in the vascular of sunflower petioles was increased when Zn was applied with other microelements with EDTA (commercial product Kick-Off) as compared with an equimolar concentration of ZnSO₄ alone. The addition of macronutrients N, P, K (commercial product CleanStart) to the Kick-Off Zn fertilizer, further increased vascular system Zn concentrations whilemore » the addition of the microbially derived organic biostimulant “GroZyme” resulted in a remarkable enhancement of Zn concentrations in the petiole vascular system. The study provides direct visualized evidence for phloem transport of foliar applied Zn out of sites of application in plants by using μ-XRF technique, and suggests that the formulation of the foliar applied Zn and the addition of the organic biostimulant GroZyme increases the mobility of Zn following its absorption by the leaf of sunflower.« less

Authors:
 [1];  [2];  [1];  [3];  [3];  [2];  [4];  [3]
  1. Zhejiang Univ., Hangzhou (China). MOE Key Lab. of Environment Remediation and Ecological Health; Univ. of California, Davis, CA (United States). Dept. of Plant Sciences.
  2. Zhejiang Univ., Hangzhou (China). MOE Key Lab. of Environment Remediation and Ecological Health.
  3. Univ. of California, Davis, CA (United States). Dept. of Plant Sciences.
  4. Ag Spectrum Co., Vero Beach, FL (United States)
Publication Date:
Research Org.:
SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1202310
Grant/Contract Number:  
AC02-76SF00515
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Frontiers in Plant Science
Additional Journal Information:
Journal Volume: 5; Journal ID: ISSN 1664-462X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; foliar fertilizer; remobilization; sunflower; μ-XRF; zinc; microbial fermentation; biostimulant

Citation Formats

Tian, Shengke, Lu, Lingli, Xie, Ruohan, Zhang, Minzhe, Jernstedt, Judith A., Hou, Dandi, Ramsier, Cliff, and Brown, Patrick H. Supplemental macronutrients and microbial fermentation products improve the uptake and transport of foliar applied zinc in sunflower (Helianthus annuus L.) plants. Studies utilizing micro X-ray florescence. United States: N. p., 2015. Web. doi:10.3389/fpls.2014.00808.
Tian, Shengke, Lu, Lingli, Xie, Ruohan, Zhang, Minzhe, Jernstedt, Judith A., Hou, Dandi, Ramsier, Cliff, & Brown, Patrick H. Supplemental macronutrients and microbial fermentation products improve the uptake and transport of foliar applied zinc in sunflower (Helianthus annuus L.) plants. Studies utilizing micro X-ray florescence. United States. https://doi.org/10.3389/fpls.2014.00808
Tian, Shengke, Lu, Lingli, Xie, Ruohan, Zhang, Minzhe, Jernstedt, Judith A., Hou, Dandi, Ramsier, Cliff, and Brown, Patrick H. 2015. "Supplemental macronutrients and microbial fermentation products improve the uptake and transport of foliar applied zinc in sunflower (Helianthus annuus L.) plants. Studies utilizing micro X-ray florescence". United States. https://doi.org/10.3389/fpls.2014.00808. https://www.osti.gov/servlets/purl/1202310.
@article{osti_1202310,
title = {Supplemental macronutrients and microbial fermentation products improve the uptake and transport of foliar applied zinc in sunflower (Helianthus annuus L.) plants. Studies utilizing micro X-ray florescence},
author = {Tian, Shengke and Lu, Lingli and Xie, Ruohan and Zhang, Minzhe and Jernstedt, Judith A. and Hou, Dandi and Ramsier, Cliff and Brown, Patrick H.},
abstractNote = {Enhancing nutrient uptake and the subsequent elemental transport from the sites of application to sites of utilization is of great importance to the science and practical field application of foliar fertilizers. The aim of this study was to investigate the mobility of various foliar applied zinc (Zn) formulations in sunflower (Helianthus annuus L.) and to evaluate the effects of the addition of an organic biostimulant on phloem loading and elemental mobility. This was achieved by application of foliar formulations to the blade of sunflower (H. annuus L.) and high-resolution elemental imaging with micro X-ray fluorescence (μ-XRF) to visualize Zn within the vascular system of the leaf petiole. Although no significant increase of total Zn in petioles was determined by inductively-coupled plasma mass-spectrometer, μ-XRF elemental imaging showed a clear enrichment of Zn in the vascular tissues within the sunflower petioles treated with foliar fertilizers containing Zn. The concentration of Zn in the vascular of sunflower petioles was increased when Zn was applied with other microelements with EDTA (commercial product Kick-Off) as compared with an equimolar concentration of ZnSO₄ alone. The addition of macronutrients N, P, K (commercial product CleanStart) to the Kick-Off Zn fertilizer, further increased vascular system Zn concentrations while the addition of the microbially derived organic biostimulant “GroZyme” resulted in a remarkable enhancement of Zn concentrations in the petiole vascular system. The study provides direct visualized evidence for phloem transport of foliar applied Zn out of sites of application in plants by using μ-XRF technique, and suggests that the formulation of the foliar applied Zn and the addition of the organic biostimulant GroZyme increases the mobility of Zn following its absorption by the leaf of sunflower.},
doi = {10.3389/fpls.2014.00808},
url = {https://www.osti.gov/biblio/1202310}, journal = {Frontiers in Plant Science},
issn = {1664-462X},
number = ,
volume = 5,
place = {United States},
year = {Wed Jan 21 00:00:00 EST 2015},
month = {Wed Jan 21 00:00:00 EST 2015}
}

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Cited by: 23 works
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Works referenced in this record:

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Comparative movement of labelled nitrogen and zinc in 1-year-old peach [ Prunus persica (L.) Batsch] trees following late-season foliar application
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Improved nitrogen nutrition enhances root uptake, root-to-shoot translocation and remobilization of zinc (65Zn) in wheat
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Comportamento vegetativo e produtividade de girassol em função do arranjo espacial das plantas
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Zinc Application Effects on Yield and Seed Oil Content of Sunflower Grown on a Saline Calcareous Soil
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Rapeseed and sunflower meal: a review on biotechnology status and challenges
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Using synchrotron X-ray fluorescence microprobes in the study of metal homeostasis in plants
journal, January 2009


Effects of agricultural biostimulants on soil microbial activity and nitrogen dynamics
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Spatial imaging of Zn and other elements in Huanglongbing-affected grapefruit by synchrotron-based micro X-ray fluorescence investigation
journal, January 2014


Distribution and Transport of Foliar Applied Zinc in Pistachio
journal, July 1999


Isotope ratio determination in boron analysis
journal, December 1998


Rapeseed and sunflower meal: a review on biotechnology status and challenges
journal, July 2012


Bacterial siderophores efficiently provide iron to iron-starved tomato plants in hydroponics culture
journal, June 2013


Biofortification of rice grain with zinc through zinc fertilization in different countries
journal, March 2012


Agricultural uses of plant biostimulants
journal, May 2014


Imaging element distribution and speciation in plant cells
journal, March 2014


The influence of two agricultural biostimulants on nitrogen transformations, microbial activity, and plant growth in soil microcosms
journal, January 2003


Effects of agricultural biostimulants on soil microbial activity and nitrogen dynamics
journal, March 2002


Effects of Nitrogen on the Distribution and Chemical Speciation of Iron and Zinc in Pearling Fractions of Wheat Grain
journal, May 2014


Zinc Application Effects on Yield and Seed Oil Content of Sunflower Grown on a Saline Calcareous Soil
journal, October 2006


Spatial imaging of Zn and other elements in Huanglongbing-affected grapefruit by synchrotron-based micro X-ray fluorescence investigation
journal, January 2014


Improved nitrogen nutrition enhances root uptake, root-to-shoot translocation and remobilization of zinc (65Zn) in wheat
journal, October 2010


Efficient xylem transport and phloem remobilization of Zn in the hyperaccumulator plant species Sedum alfredii
journal, February 2013


Foliar Absorption of Mineral Nutrients
journal, June 1959


From plant surface to plant metabolism: the uncertain fate of foliar-applied nutrients
journal, January 2013


The influence of inorganic nitrogen fertilizer forms on micronutrient retranslocation and accumulation in grains of winter wheat
journal, January 2013


Works referencing / citing this record:

2015 Atomic Spectrometry Update – a review of advances in X-ray fluorescence spectrometry and their applications
journal, January 2015


Recent advances in LIBS and XRF for the analysis of plants
journal, January 2018

  • Arantes de Carvalho, Gabriel Gustinelli; Bueno Guerra, Marcelo Braga; Adame, Andressa
  • Journal of Analytical Atomic Spectrometry, Vol. 33, Issue 6
  • https://doi.org/10.1039/c7ja00293a

Biostimulants in agriculture
journal, August 2015


Developing Biostimulants From Agro-Food and Industrial By-Products
journal, October 2018


Laboratory Microprobe X-Ray Fluorescence in Plant Science: Emerging Applications and Case Studies
journal, November 2018


Simplifying Sample Preparation for Soil Fertility Analysis by X-ray Fluorescence Spectrometry
journal, November 2019


Foliar application of zinc sulphate and zinc EDTA to wheat leaves: differences in mobility, distribution, and speciation
journal, June 2018


Biostimulants in Plant Science: A Global Perspective
journal, January 2017


Developing Biostimulants From Agro-Food and Industrial By-Products
journal, October 2018


Simplifying Sample Preparation for Soil Fertility Analysis by X-ray Fluorescence Spectrometry
journal, November 2019