Hydrogel nanoparticle based immunoassay
An immunoassay device incorporating porous polymeric capture nanoparticles within either the sample collection vessel or pre-impregnated into a porous substratum within fluid flow path of the analytical device is presented. This incorporation of capture particles within the immunoassay device improves sensitivity while removing the requirement for pre-processing of samples prior to loading the immunoassay device. A preferred embodiment is coreshell bait containing capture nanoparticles which perform three functions in one step, in solution: a) molecular size sieving, b) target analyte sequestration and concentration, and c) protection from degradation. The polymeric matrix of the capture particles may be made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. This device is useful for point of care diagnostic assays for biomedical applications and as field deployable assays for environmental, pathogen and chemical or biological threat identification.
- Research Organization:
- George Mason Research Foundation, Inc., Fairfax, VA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- FC52-04NA25455
- Assignee:
- George Mason Research Foundation, Inc. (Fairfax, VA)
- Patent Number(s):
- 9,012,240
- Application Number:
- 13/061,507
- OSTI ID:
- 1178255
- Resource Relation:
- Patent File Date: 2009 Aug 26
- Country of Publication:
- United States
- Language:
- English
Smart Hydrogel Particles: Biomarker Harvesting: One-Step Affinity Purification, Size Exclusion, and Protection against Degradation
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journal | January 2008 |
Ligand-Functionalized Core/Shell Microgels with Permselective Shells
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journal | December 2004 |
Dry chemistry, lateral flow-reconstituted chromatographic enzyme-driven assays
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patent | September 2008 |
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