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Title: Methods for sequencing GC-rich and CCT repeat DNA templates

Patent ·
OSTI ID:1176115

The present invention is directed to a PCR-based method of cycle sequencing DNA and other polynucleotide sequences having high CG content and regions of high GC content, and includes for example DNA strands with a high Cytosine and/or Guanosine content and repeated motifs such as CCT repeats.

Research Organization:
Los Alamos National Security, LLC, Los Alamos, NM (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG02-98ER62647
Assignee:
Los Alamos National Security, LLC (Los Alamos, NM)
Patent Number(s):
7,179,602
Application Number:
10/656,358
OSTI ID:
1176115
Country of Publication:
United States
Language:
English

References (22)

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DNA sequencing by primer walking with strings of contiguous hexamers journal December 1992
Incorporation of 7-deaza dGTP during the amplification step in the polymerase chain reaction procedure improves subsequent DNA sequencing journal January 1990
Maturational patterns in early hominids journal August 1987
Betaine Improves the PCR Amplification of GC-Rich DNA Sequences journal October 1997
Base-Calling of Automated Sequencer Traces Using Phred. II. Error Probabilities journal March 1998
Base-Calling of Automated Sequencer Traces Using Phred.  I. Accuracy Assessment journal March 1998
PCR with 7-Deaza-2′-Deoxyguanosine Triphosphate book January 1990
A single residue in DNA polymerases of the Escherichia coli DNA polymerase I family is critical for distinguishing between deoxy- and dideoxyribonucleotides. journal July 1995
A new method for sequencing DNA. journal February 1977
The synthesis of oligonucleotides containing an aliphatic amino group at the 5′ terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis journal January 1985
Improved double-stranded DNA sequencing using the linear polymerase chain reaction journal January 1989
A strategy for high-volume sequencing of cosmid DNAs: random and directed priming with a library of oligonucleotides. journal September 1989
The enhancement of PCR amplification by low molecular-weight sulfones journal August 2001
A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides journal October 1987
DNA sequencing by capillary electrophoresis with replaceable linear polyacrylamide and laser-induced fluorescence detection journal October 1993
Use of Dimethyl Sulfoxide to Improve Fluorescent, Taq Cycle Sequencing book January 1994
A fast and simple method for sequencing DNA cloned in the single-stranded bacteriophage M13 journal March 1979
Capillary gel electrophoresis for rapid, high resolution DNA sequencing journal January 1990
Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content. journal July 1996
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing journal October 1980
Multiplex DNA sequencing journal April 1988

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES