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Title: Engineering disease resistance with pectate lyase-like genes

Patent ·
OSTI ID:1175262

A mutant gene coding for pectate lyase and homologs thereof is provided, which when incorporated in transgenic plants effect an increased level disease resistance in such plants. Also is provided the polypeptide sequence for the pectate lyase of the present invention. Methods of obtaining the mutant gene, producing transgenic plants which include the nucleotide sequence for the mutant gene and producing improved disease resistance in a crop of such transgenic plants are also provided.

Research Organization:
Carnegie Inst. of Washington, Washington, DC (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG02-94ER20133
Assignee:
Carnegie Institution of Washington (Washington, DC)
Patent Number(s):
6,864,404
Application Number:
09/684,960
OSTI ID:
1175262
Country of Publication:
United States
Language:
English

References (16)

Cry j I, a major allergen of Japanese cedar pollen, has pectate lyase enzyme activity journal January 1995
Plant Disease Susceptibility Genes? journal September 2002
Genetics of Powdery Mildew Resistance in Barley journal January 1994
Cloning, molecular characterization and expression pattern of a strawberry ripening-specific cDNA with sequence homology to pectate lyase from higher plants journal August 1997
Constitutive expression of pathogenesis-related proteins PR-1, GRP, and PR-S in tobacco has no effect on virus infection. journal March 1989
The Identity of Plant Glutamate Receptors journal May 2001
Le multimédia peut-il aider à réduire la dissonance cognitive ? journal January 1998
Powers and Pitfalls in Sequence Analysis: The 70% Hurdle journal April 2000
Systemic Acquired Resistance. journal October 1996
PMR6 , a Pectate Lyase–Like Gene Required for Powdery Mildew Susceptibility in Arabidopsis journal September 2002
Genetic characterization of five powdery mildew disease resistance loci in Arabidopsis thaliana journal March 1996
Biological effects of pectic fragments in plant cells journal September 1994
Small oligomers of galacturonic acid are endogenous suppressors of disease resistance reactions in wheat leaves journal May 1999
The cpr5 mutant of Arabidopsis expresses both NPR1-dependent and NPR1-independent resistance. journal September 1997
A simple and rapid method for the preparation of plant genomic DNA for PCR analysis journal January 1991
Extracellular Enzymes and Pathogenesis of Soft-Rot Erwinia journal September 1994

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