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Title: Method for chromium analysis and speciation

Patent ·
OSTI ID:1175109

A method of detecting a metal in a sample comprising a plurality of metal is disclosed. The method comprises providing the sample comprising a metal to be detected. The sample is added to a reagent solution comprising an enzyme and a substrate, where the enzyme is inhibited by the metal to be detected. An array of chelating agents is used to eliminate the inhibitory effects of additional metals in the sample. An enzymatic activity in the sample is determined and compared to an enzymatic activity in a control solution to detect the metal to be detected. A method of determining a concentration of the metal in the sample is also disclosed. A method of detecting a valence state of a metal is also disclosed.

Research Organization:
Idaho National Laboratory (INL), Idaho Falls, ID (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC07-99ID13727
Assignee:
Bechtel BWXT Idaho, LLC (Idaho Falls, ID)
Patent Number(s):
6,811,997
Application Number:
10/189,035
OSTI ID:
1175109
Country of Publication:
United States
Language:
English

References (3)

Phytochelatins protect plant enzymes from heavy metal poisoning journal August 1992
In vitro alleviation of heavy metal-induced enzyme inhibition by proline journal November 1998
A Spectrophotometric Assay for Nitrate Using NADPH Oxidation by Aspergillus Nitrate Reductase journal August 1993