Chemical inducible promoter used to obtain transgenic plants with a silent marker

Aoyama, Takashi; Zuo, Jianru; Chua, Nam-Hai

Title: Chemical inducible promoter used to obtain transgenic plants with a silent marker

Patent · Tue Aug 31 00:00:00 EDT 2004

OSTI ID:1175006

Aoyama, Takashi; Zuo, Jianru; Chua, Nam-Hai

A chemically inducible promoter is described that may be used to transform plants, including tobacco and lettuce, with genes which are easily regulatable by adding the plants or plant cells to a medium containing an inducer of the promoter or by removing the plants or plant cells from such medium. The promoter described is one that is inducible by a glucocorticoid which is not endogenous to plants. Such promoters may be used with a variety of genes such as ipt or knotted1 to induce shoot formation in the presence of a glucocorticoid. The promoter may also be used with antibiotic or herbicide resistance genes which are then regulatable by the presence or absence of inducer rather than being constitutive. Other examples of genes which may be placed under the control of the inducible promoter are also presented.

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Research Organization:: The Rockefeller University, New York, NY (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-94ER20142

Assignee:: The Rockefeller University (New York, NY)

Patent Number(s):: 6,784,340

Application Number:: 09/438,392

OSTI ID:: 1175006

Country of Publication:: United States

Language:: English

References (73)

A glucocorticoid-inducible transcription system causes severe growth defects in Arabidopsis and induces defense-related genes Kang, Hong-Gu; Fang, Yiwen; Singh, Karam B. The Plant Journal, Vol. 20, Issue 1 https://doi.org/10.1046/j.1365-313X.1999.00575.x	journal	October 1999
Specific DNA binding of GAL4, a positive regulatory protein of yeast Giniger, Edward; Varnum, Susan M.; Ptashne, Mark Cell, Vol. 40, Issue 4 https://doi.org/10.1016/0092-8674(85)90336-8	journal	April 1985
Primary structure of the Saccharomyces cerevisiae GAL4 gene. Laughon, A.; Gesteland, R. F. Molecular and Cellular Biology, Vol. 4, Issue 2 https://doi.org/10.1128/MCB.4.2.260	journal	February 1984
Genes Specifying Auxin and Cytokinin Biosynthesis in Prokaryotes Morris, Roy O. Plant Hormones https://doi.org/10.1007/978-94-011-0473-9_15	book	January 1995
Cytokinin-mediated insect resistance in Nicotiana plants transformed with the ipt gene Smigocki, A.; Neal, J. W.; McCanna, I. Plant Molecular Biology, Vol. 23, Issue 2 https://doi.org/10.1007/BF00029008	journal	October 1993
Transgenic Plants in Hormone Biology Klee, Harry J.; Lanahan, Michael B. Plant Hormones https://doi.org/10.1007/978-94-011-0473-9_16	book	January 1995
Stringent repression and homogeneous de-repression by tetracycline of a modified CaMV 35S promoter in intact transgenic tobacco plants. Gatz, C.; Frohberg, C.; Wendenburg, R. The Plant Journal, Vol. 2, Issue 3 https://doi.org/10.1046/j.1365-313X.1992.t01-37-00999.x	journal	May 1992
Altered morphology in transgenic tobacco plants that overproduce cytokinins in specific tissues and organs Li, Yi; Hagen, Gretchen; Guilfoyle, Tom J. Developmental Biology, Vol. 153, Issue 2 https://doi.org/10.1016/0012-1606(92)90123-X	journal	October 1992
The Cauliflower Mosaic Virus 35S Promoter: Combinatorial Regulation of Transcription in Plants Benfey, P. N.; Chua, N. -H. Science, Vol. 250, Issue 4983 https://doi.org/10.1126/science.250.4983.959	journal	November 1990
Genetic complementation of a glucocorticoid receptor deficiency by expression of cloned receptor cDNA Miesfeld, Roger; Rusconi, Sandro; Godowski, Paul J. Cell, Vol. 46, Issue 3 https://doi.org/10.1016/0092-8674(86)90659-8	journal	August 1986
A novel circadian phenotype based on firefly luciferase expression in transgenic plants. Millar, A. J.; Short, S. R.; Chua, N. H. The Plant Cell, Vol. 4, Issue 9 https://doi.org/10.1105/tpc.4.9.1075	journal	September 1992
Construction of Gene Expression System in Cultured Tobacco Cellsa Shinmyo, A.; Yoshida, K.; Kasai, T. Annals of the New York Academy of Sciences, Vol. 782, Issue 1 https://doi.org/10.1111/j.1749-6632.1996.tb40551.x	journal	May 1996
DNA sequence of the herpes simplex virus type 1 gene whose product is responsible for transcriptional activation of immediate early promoters Dalrymple, M. A.; McGeoch, D. J.; Davison, A. J. Nucleic Acids Research, Vol. 13, Issue 21 https://doi.org/10.1093/nar/13.21.7865	journal	January 1985
Nucleotide sequence of the lexA gene of E. coli Horii, Toshihiro; Ogawa, Tomoko; Ogawa, Hideyuki Cell, Vol. 23, Issue 3 https://doi.org/10.1016/0092-8674(81)90432-3	journal	March 1981
Agrobacterium-Mediated Plant Transformation and its Further Applications to Plant Biology Klee, H.; Horsch, R.; Rogers, S. Annual Review of Plant Physiology, Vol. 38, Issue 1 https://doi.org/10.1146/annurev.pp.38.060187.002343	journal	June 1987
A selective transcriptional induction system for mammalian cells based on Gal4-estrogen receptor fusion proteins. Braselmann, S.; Graninger, P.; Busslinger, M. Proceedings of the National Academy of Sciences, Vol. 90, Issue 5 https://doi.org/10.1073/pnas.90.5.1657	journal	March 1993
Chemically inducible promoters in transgenic plants Gatz, Christine Current Opinion in Biotechnology, Vol. 7, Issue 2 https://doi.org/10.1016/S0958-1669(96)80008-5	journal	April 1996
Multiple resistance to sulfonylureas and imidazolinones conferred by an acetohydroxyacid synthase gene with separate mutations for selective resistance Hattori, Jiro; Rutledge, Robert; Labbé, Hélène Molecular and General Genetics MGG, Vol. 232, Issue 2 https://doi.org/10.1007/BF00279993	journal	March 1992
T-DNA of Agrobacterium tumefaciens encodes an enzyme of cytokinin biosynthesis. Akiyoshi, D. E.; Klee, H.; Amasino, R. M. Proceedings of the National Academy of Sciences, Vol. 81, Issue 19 https://doi.org/10.1073/pnas.81.19.5994	journal	October 1984
An acetohydroxy acid synthase mutant reveals a single site involved in multiple herbicide resistance Hattori, Jiro; Brown, Douglas; Mourad, George Molecular and General Genetics MGG, Vol. 246, Issue 4 https://doi.org/10.1007/BF00290445	journal	July 1995
Expression of a bacterial gene in transgenic tobacco plants confers resistance to the herbicide 2,4-dichlorophenoxyacetic acid Lyon, Bruce R.; Llewellyn, Danny J.; Huppatz, John L. Plant Molecular Biology, Vol. 13, Issue 5 https://doi.org/10.1007/BF00027313	journal	November 1989
Ectopic Expression of a Tobacco Homeobox Gene, NTH15, Dramatically Alters Leaf Morphology and Hormone Levels in Transgenic Tobacco Tamaoki, M.; Kusaba, S.; Kano-Murakami, Y. Plant and Cell Physiology, Vol. 38, Issue 8 https://doi.org/10.1093/oxfordjournals.pcp.a029252	journal	January 1997
Ecdysone agonist inducible transcription in transgenic tobacco plants Martinez, Alberto; Sparks, Caroline; Hart, Cliff A. The Plant Journal, Vol. 19, Issue 1 https://doi.org/10.1046/j.1365-313X.1999.00504.x	journal	July 1999
A knotted1-like homeobox gene in Arabidopsis is expressed in the vegetative meristem and dramatically alters leaf morphology when overexpressed in transgenic plants. Lincoln, C.; Long, J.; Yamaguchi, J. The Plant Cell, Vol. 6, Issue 12 https://doi.org/10.1105/tpc.6.12.1859	journal	December 1994
Glucocorticoid-inducible expression of a bacterial avirulence gene in transgenic Arabidopsis induces hypersensitive cell death: Glucocorticoid-inducible cell death McNellis, Timothy W.; Mudgett, Mary Beth; Li, Karen The Plant Journal, Vol. 14, Issue 2 https://doi.org/10.1046/j.1365-313X.1998.00106.x	journal	April 1998
Epidermal cell fate determination in Arabidopsis: patterns defined by a steroid-inducible regulator Lloyd, A.; Schena, M.; Walbot, V. Science, Vol. 266, Issue 5184 https://doi.org/10.1126/science.7939683	journal	October 1994
Cytokinin gene fused with a strong promoter enhances shoot organogenesis and zeatin levels in transformed plant cells Smigocki, A. C.; Owens, L. D. Proceedings of the National Academy of Sciences, Vol. 85, Issue 14 https://doi.org/10.1073/pnas.85.14.5131	journal	July 1988
An estrogen receptor-based transactivator XVE mediates highly inducible gene expression in transgenic plants Zuo, Jianru; Niu, Qi-Wen; Chua, Nam-Hai The Plant Journal, Vol. 24, Issue 2 https://doi.org/10.1046/j.1365-313x.2000.00868.x	journal	October 2000
Sequence and expression of human estrogen receptor complementary DNA Greene, G.; Gilna, P.; Waterfield, M. Science, Vol. 231, Issue 4742 https://doi.org/10.1126/science.3753802	journal	March 1986
Use of bar as a selectable marker gene and for the production of herbicide-resistant rice plants from protoplasts Rathore, Keerti S.; Chowdhury, Vijay K.; Hodges, Thomas K. Plant Molecular Biology, Vol. 21, Issue 5 https://doi.org/10.1007/BF00027118	journal	March 1993
GAL4-VP16 is an unusually potent transcriptional activator Sadowski, Ivan; Ma, Jun; Triezenberg, Steve Nature, Vol. 335, Issue 6190 https://doi.org/10.1038/335563a0	journal	October 1988
Steroid-binding domains for regulating the functions of heterologous proteins in cis Picard, Didier Trends in Cell Biology, Vol. 3, Issue 8 https://doi.org/10.1016/0962-8924(93)90057-8	journal	August 1993
From tumour to tuber; tumour cell characteristics and chromosome numbers of crown gall-derived tetraploid potato plants (Solanum tuberosum cv. ‘Maris Bard’) Ooms, G.; Karp, A.; Roberts, J. Theoretical and Applied Genetics, Vol. 66, Issue 2 https://doi.org/10.1007/BF00265193	journal	July 1983
A movable and regulable inactivation function within the steroid binding domain of the glucocorticoid receptor Picard, Didier; Salser, Stephen J.; Yamamoto, Keith R. Cell, Vol. 54, Issue 7 https://doi.org/10.1016/0092-8674(88)90122-5	journal	September 1988
Alterations of Endogenous Cytokinins in Transgenic Plants Using a Chimeric Isopentenyl Transferase Gene. Medford, J. I.; Horgan, R.; El-Sawi, Z. The Plant Cell https://doi.org/10.1105/tpc.1.4.403	journal	April 1989
Tissue-specific and light-regulated expression of a pea nuclear gene encoding the small subunit of ribulose-1,5-bisphosphate carboxylase. Coruzzi, G.; Broglie, R.; Edwards, C. The EMBO Journal, Vol. 3, Issue 8 https://doi.org/10.1002/j.1460-2075.1984.tb02031.x	journal	August 1984
Cytokinin content and tissue distribution in plants transformed by a reconstructed isopentenyl transferase gene Smigocki, Ann C. Plant Molecular Biology, Vol. 16, Issue 1 https://doi.org/10.1007/BF00017921	journal	January 1991
Functional dissection of VP16, the trans-activator of herpes simplex virus immediate early gene expression. Triezenberg, S. J.; Kingsbury, R. C.; McKnight, S. L. Genes & Development, Vol. 2, Issue 6 https://doi.org/10.1101/gad.2.6.718	journal	June 1988
Gene regulation by steroid hormones Beato, Miguel Cell, Vol. 56, Issue 3 https://doi.org/10.1016/0092-8674(89)90237-7	journal	February 1989
Control of Morphogenesis in in Vitro Cultures Tran Thanh Van, K. M. Annual Review of Plant Physiology, Vol. 32, Issue 1 https://doi.org/10.1146/annurev.pp.32.060181.001451	journal	June 1981
Inhibition of Leaf Senescence by Autoregulated Production of Cytokinin Gan, S.; Amasino, R. M. Science, Vol. 270, Issue 5244 https://doi.org/10.1126/science.270.5244.1986	journal	December 1995
Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter Odell, Joan T.; Nagy, Ferenc; Chua, Nam-Hai Nature, Vol. 313, Issue 6005, p. 810-812 https://doi.org/10.1038/313810a0	journal	February 1985
Resistance to hygromycin B: A new marker for plant transformation studies Waldron, C.; Murphy, E. B.; Roberts, J. L. Plant Molecular Biology, Vol. 5, Issue 2 https://doi.org/10.1007/BF00020092	journal	January 1985
Genetic transformation mediated byAgrobacterium tumefaciens of florists' chrysanthemum (Dendranthema xgrandiflorum) cultivar ‘Peach Margaret’ Boase, M. R.; Bradley, J. M.; Borst, N. K. In Vitro Cellular & Developmental Biology - Plant, Vol. 34, Issue 1 https://doi.org/10.1007/BF02823122	journal	January 1998
Conditional transgenic expression of the ipt gene indicates a function for cytokinins in paracrine signaling in whole tobacco plants Faiss, Martin; Zalubilova, Jana; Strnad, Miroslav The Plant Journal, Vol. 12, Issue 2 https://doi.org/10.1046/j.1365-313X.1997.12020401.x	journal	August 1997
Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans. Napoli, C.; Lemieux, C.; Jorgensen, R. The Plant Cell https://doi.org/10.1105/tpc.2.4.279	journal	April 1990
Separation of DNA binding from the transcription-activating function of a eukaryotic regulatory protein Keegan, L.; Gill, G.; Ptashne, M. Science, Vol. 231, Issue 4739 https://doi.org/10.1126/science.3080805	journal	February 1986
Identification of a cloned cytokinin biosynthetic gene Barry, G. F.; Rogers, S. G.; Fraley, R. T. Proceedings of the National Academy of Sciences, Vol. 81, Issue 15 https://doi.org/10.1073/pnas.81.15.4776	journal	August 1984
Light-induced expression of ipt from Agrobacterium tumefaciens results in cytokinin accumulation and osmotic stress symptoms in transgenic tobacco Thomas, John C.; Smigocki, Ann C.; Bohnert, Hans J. Plant Molecular Biology, Vol. 27, Issue 2 https://doi.org/10.1007/BF00020179	journal	January 1995
Drosophila TAFII40 interacts with both a VP16 activation domain and the basal transcription factor TFIIB Goodrich, James A.; Hoey, Timothy; Thut, Catherine J. Cell, Vol. 75, Issue 3 https://doi.org/10.1016/0092-8674(93)90386-5	journal	November 1993
Chemical Control of gene Expression Gatz, C. Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 48, Issue 1 https://doi.org/10.1146/annurev.arplant.48.1.89	journal	June 1997
A GFP-mouse talin fusion protein labels plant actin filamentsin vivoand visualizes the actin cytoskeleton in growing pollen tubes Kost, Benedikt; Spielhofer, Pius; Chua, Nam-Hai The Plant Journal, Vol. 16, Issue 3 https://doi.org/10.1046/j.1365-313x.1998.00304.x	journal	November 1998
A steroid-inducible gene expression system for plant cells. Schena, M.; Lloyd, A. M.; Davis, R. W. Proceedings of the National Academy of Sciences, Vol. 88, Issue 23 https://doi.org/10.1073/pnas.88.23.10421	journal	December 1991
Herbicide Safener-Inducible Gene Expression in Arabidopsis thaliana de Veylder, L.; van Montagu, M.; Inze, D. Plant and Cell Physiology, Vol. 38, Issue 5 https://doi.org/10.1093/oxfordjournals.pcp.a029206	journal	January 1997
Inducible isopentenyl transferase as a high-efficiency marker for plant transformation Kunkel, Tim; Niu, Qi-Wen; Chan, Yang-Sun Nature Biotechnology, Vol. 17, Issue 9 https://doi.org/10.1038/12914	journal	September 1999
T-DNA of the Agrobacterium TI and RI Plasmids Bevan, M. W.; Chilton, M. Annual Review of Genetics, Vol. 16, Issue 1 https://doi.org/10.1146/annurev.ge.16.120182.002041	journal	December 1982
Functional dissection of the hormone and DNA binding activities of the glucocorticoid receptor. Rusconi, S.; Yamamoto, K. R. The EMBO Journal, Vol. 6, Issue 5 https://doi.org/10.1002/j.1460-2075.1987.tb02369.x	journal	May 1987
Transcriptional activator TGV mediates dexamethasone-inducible and tetracycline-inactivatable gene expression Bohner, Steffen; Lenk, Ingo; Rieping, Mechthild The Plant Journal, Vol. 19, Issue 1 https://doi.org/10.1046/j.1365-313X.1999.00503.x	journal	July 1999
CKI1, a Histidine Kinase Homolog Implicated in Cytokinin Signal Transduction Kakimoto, Tatsuo Science, Vol. 274, Issue 5289 https://doi.org/10.1126/science.274.5289.982	journal	November 1996
Analysis of Cytokinin Metabolism in ipt Transgenic Tobacco by Liquid Chromatography-Tandem Mass Spectrometry Redig, P.; Schmulling, T.; Van Onckelen, H. Plant Physiology, Vol. 112, Issue 1 https://doi.org/10.1104/pp.112.1.141	journal	September 1996
Cotton plants transformed with a bacterial degradation gene are protected from accidental spray drift damage by the herbicide 2,4-dichlorophenoxyacetic acid Lyon, Bruce R.; Cousins, Yvonne L.; Llewellyn, Danny J. Transgenic Research, Vol. 2, Issue 3 https://doi.org/10.1007/BF01972610	journal	May 1993
Herbicide resistance due to amplification of a mutant acetohydroxyacid synthase gene Harms, Christian T.; Armour, Susan L.; DiMaio, Joseph J. Molecular and General Genetics MGG, Vol. 233, Issue 3 https://doi.org/10.1007/BF00265440	journal	June 1992
Ectopic expression of the Arabidopsis transcriptional activator Athb-1 alters leaf cell fate in tobacco. Aoyama, T.; Dong, C. H.; Wu, Y. The Plant Cell, Vol. 7, Issue 11 https://doi.org/10.1105/tpc.7.11.1773	journal	November 1995
Expression Profiling of the Maize Flavonoid Pathway Genes Controlled by Estradiol-Inducible Transcription Factors CRC and P Bruce, Wesley; Folkerts, Otto; Garnaat, Carl The Plant Cell, Vol. 12, Issue 1 https://doi.org/10.1105/tpc.12.1.65	journal	January 2000
Selection of marker-free transgenic plants using the isopentenyl transferase gene Ebinuma, H.; Sugita, K.; Matsunaga, E. Proceedings of the National Academy of Sciences, Vol. 94, Issue 6 https://doi.org/10.1073/pnas.94.6.2117	journal	March 1997
A G-box motif (GCCACGTGCC) tetramer confers high-level constitutive expression in dicot and monocot plants Ishige, Fumiharu; Takaichi, Miyuki; Foster, Randy The Plant Journal, Vol. 18, Issue 4 https://doi.org/10.1046/j.1365-313X.1999.00456.x	journal	May 1999
Isolation, expression and phylogenetic inheritance of an acetolactate synthase gene from Brassica napus Wiersma, P. A.; Schmiemann, M. G.; Condie, J. A. Molecular and General Genetics MGG, Vol. 219, Issue 3 https://doi.org/10.1007/BF00259614	journal	November 1989
Cytokinin-to-Auxin Ratios and Morphology of Shoots and Tissues Transformed by a Chimeric Isopentenyl Transferase Gene Smigocki, Ann C.; Owens, Lowell D. Plant Physiology, Vol. 91, Issue 3 https://doi.org/10.1104/pp.91.3.808	journal	November 1989
Binding of general transcription factor TFIIB to an acidic activating region Lin, Young-Sun; Ha, Ilho; Maldonado, Edio Nature, Vol. 353, Issue 6344 https://doi.org/10.1038/353569a0	journal	October 1991
KNAT1 induces lobed leaves with ectopic meristems when overexpressed in Arabidopsis. Chuck, G.; Lincoln, C.; Hake, S. The Plant Cell, Vol. 8, Issue 8 https://doi.org/10.1105/tpc.8.8.1277	journal	August 1996
Organization of the lexA gene of Escherichia coli and nucleotide sequence of the regulatory region Miki, Toru; Ebina, Yousuke; Kishi, Fumio Nucleic Acids Research, Vol. 9, Issue 3 https://doi.org/10.1093/nar/9.3.529	journal	January 1981
Controlled Cytokinin Production in Transgenic Tobacco Using a Copper-Inducible Promoter McKenzie, Marian Jane; Mett, Vadim; Reynolds, Paul Hugh Stewart Plant Physiology, Vol. 116, Issue 3 https://doi.org/10.1104/pp.116.3.969	journal	March 1998
Promoters that respond to chemical inducers Gatz, Christiane; Lenk, Ingo Trends in Plant Science, Vol. 3, Issue 9 https://doi.org/10.1016/S1360-1385(98)01287-4	journal	September 1998

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