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Title: Microfluidic systems and methods of transport and lysis of cells and analysis of cell lysate

Abstract

Microfluidic systems and methods are disclosed which are adapted to transport and lyse cellular components of a test sample for analysis. The disclosed microfluidic systems and methods, which employ an electric field to rupture the cell membrane, cause unusually rapid lysis, thereby minimizing continued cellular activity and resulting in greater accuracy of analysis of cell processes.

Inventors:
; ; ;
Publication Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175002
Patent Number(s):
6,783,647
Application Number:
10/041,821
Assignee:
UT-Battelle, LLC (Oak Ridge, TN)
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
30 DIRECT ENERGY CONVERSION

Citation Formats

Culbertson, Christopher T., Jacobson, Stephen C., McClain, Maxine A., and Ramsey, J. Michael. Microfluidic systems and methods of transport and lysis of cells and analysis of cell lysate. United States: N. p., 2004. Web.
Culbertson, Christopher T., Jacobson, Stephen C., McClain, Maxine A., & Ramsey, J. Michael. Microfluidic systems and methods of transport and lysis of cells and analysis of cell lysate. United States.
Culbertson, Christopher T., Jacobson, Stephen C., McClain, Maxine A., and Ramsey, J. Michael. 2004. "Microfluidic systems and methods of transport and lysis of cells and analysis of cell lysate". United States. https://www.osti.gov/servlets/purl/1175002.
@article{osti_1175002,
title = {Microfluidic systems and methods of transport and lysis of cells and analysis of cell lysate},
author = {Culbertson, Christopher T. and Jacobson, Stephen C. and McClain, Maxine A. and Ramsey, J. Michael},
abstractNote = {Microfluidic systems and methods are disclosed which are adapted to transport and lyse cellular components of a test sample for analysis. The disclosed microfluidic systems and methods, which employ an electric field to rupture the cell membrane, cause unusually rapid lysis, thereby minimizing continued cellular activity and resulting in greater accuracy of analysis of cell processes.},
doi = {},
url = {https://www.osti.gov/biblio/1175002}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Aug 31 00:00:00 EDT 2004},
month = {Tue Aug 31 00:00:00 EDT 2004}
}

Works referenced in this record:

Continuous Cell Introduction for the Analysis of Individual Cells by Capillary Electrophoresis
journal, January 2001


Electroosmotically Induced Hydraulic Pumping with Integrated Electrodes on Microfluidic Devices
journal, August 2001


Characterization of Cellular Optoporation with Distance
journal, March 2000


Effects of high electric fields on micro-organisms
journal, August 1968


Transport, Manipulation, and Reaction of Biological Cells On-Chip Using Electrokinetic Effects
journal, April 1997


Voltage-induced pore formation and hemolysis of human erythrocytes
journal, December 1977


Hemolysis of human erythrocytes by transient electric field.
journal, May 1977


Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane)
journal, October 1998