Synechococcus elongatus UTEX 2973, a fast growing cyanobacterial chassis for biosynthesis using light and CO2

Yu, Jingjie; Liberton, Michelle; Cliften, Paul F.; Head, Richard D.; Jacobs, Jon M.; Smith, Richard D.; Koppenaal, David W.; Brand, Jerry J.; Pakrasi, Himadri B.

doi:10.1038/srep08132

Title: Synechococcus elongatus UTEX 2973, a fast growing cyanobacterial chassis for biosynthesis using light and CO2

Journal Article · Fri Jan 30 00:00:00 EST 2015 · Scientific Reports

DOI:https://doi.org/10.1038/srep08132· OSTI ID:1171276

Yu, Jingjie; Liberton, Michelle; Cliften, Paul F.; Head, Richard D.; Jacobs, Jon M.; Smith, Richard D.; Koppenaal, David W.; Brand, Jerry J.; Pakrasi, Himadri B.

Photosynthetic microbes are of emerging interest as production organisms in biotechnology because they can grow autotrophically using sunlight, an abundant energy source, and CO2, a greenhouse gas. Important traits for such microbes are fast growth and amenability to genetic manipulation. Here we describe Synechococcus elongatus UTEX 2973, a unicellular cyanobacterium capable of rapid autotrophic growth, comparable to heterotrophic industrial hosts such as yeast. Synechococcus 2973 can be readily transformed for facile generation of desired knockout and knock-in mutations. Genome sequencing coupled with global proteomics studies revealed that Synechococcus 2973 is a close relative of the widely studied cyanobacterium Synechococcus elongatus PCC 7942, an organism that grows more than two times slower. A small number of nucleotide changes are the only significant differences between the genomes of these two cyanobacterial strains. Thus, our study has unraveled genetic determinants necessary for rapid growth of cyanobacterial strains of significant industrial potential.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL); Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC05-76RL01830; SC 0001035

OSTI ID:: 1171276

Report Number(s):: PNNL-SA-105375; srep08132

Journal Information:: Scientific Reports, Vol. 5; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 212 works

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