skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Increasing cell culture population doublings for long-term growth of finite life span human cell cultures

Abstract

Cell culture media formulations for culturing human epithelial cells are herein described. Also described are methods of increasing population doublings in a cell culture of finite life span human epithelial cells and prolonging the life span of human cell cultures. Using the cell culture media disclosed alone and in combination with addition to the cell culture of a compound associated with anti-stress activity achieves extended growth of pre-stasis cells and increased population doublings and life span in human epithelial cell cultures.

Inventors:
;
Publication Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1171090
Patent Number(s):
8,962,325
Application Number:
12/242,474
Assignee:
The Regents of the University of California (Oakland, CA)
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2008 Sep 30
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Stampfer, Martha R, and Garbe, James C. Increasing cell culture population doublings for long-term growth of finite life span human cell cultures. United States: N. p., 2015. Web.
Stampfer, Martha R, & Garbe, James C. Increasing cell culture population doublings for long-term growth of finite life span human cell cultures. United States.
Stampfer, Martha R, and Garbe, James C. 2015. "Increasing cell culture population doublings for long-term growth of finite life span human cell cultures". United States. https://www.osti.gov/servlets/purl/1171090.
@article{osti_1171090,
title = {Increasing cell culture population doublings for long-term growth of finite life span human cell cultures},
author = {Stampfer, Martha R and Garbe, James C},
abstractNote = {Cell culture media formulations for culturing human epithelial cells are herein described. Also described are methods of increasing population doublings in a cell culture of finite life span human epithelial cells and prolonging the life span of human cell cultures. Using the cell culture media disclosed alone and in combination with addition to the cell culture of a compound associated with anti-stress activity achieves extended growth of pre-stasis cells and increased population doublings and life span in human epithelial cell cultures.},
doi = {},
url = {https://www.osti.gov/biblio/1171090}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Feb 24 00:00:00 EST 2015},
month = {Tue Feb 24 00:00:00 EST 2015}
}

Works referenced in this record:

Telomerase modulates expression of growth-controlling genes and enhances cell proliferation
journal, April 2003


Telomerase expression in human somatic cells does not induce changes associated with a transformed phenotype
journal, January 1999


Long-term culture of adult murine epidermal keratinocytes
journal, December 2005


Absence of cancer–associated changes in human fibroblasts immortalized with telomerase
journal, January 1999


Telomerase protects developing neurons against DNA damage-induced cell death
journal, November 2001


Enhanced growth medium and method for culturing human mammary epithelial cells
patent, December 1983


Oxytocin and Oxytocin Receptors in Cancer Cells and Proliferation
journal, April 2004


Telomerase beyond telomeres
journal, August 2002


Telomerase reverse transcriptase promotes cardiac muscle cell proliferation, hypertrophy, and survival
journal, August 2001


Expression of the telomerase catalytic subunit, hTERT, induces resistance to transforming growth factor β growth inhibition in p16INK4A(−) human mammary epithelial cells
journal, April 2001