Insights into Acetate Toxicity in Zymomonas mobilis 8b using Different Substrates

Yang, Shihui; Franden, M. A.; Brown, S. D.; Chou, Y. C.; Pienkos, P. T.; Zhang, Min

doi:10.1186/s13068-014-0140-8

Title: Insights into Acetate Toxicity in Zymomonas mobilis 8b using Different Substrates

Journal Article · Tue Sep 30 00:00:00 EDT 2014 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-014-0140-8· OSTI ID:1160173

Yang, Shihui ^[1]; Franden, M. A. ^[1]; Brown, S. D. ^[2]; Chou, Y. C. ^[1]; Pienkos, P. T. ^[1]; Zhang, Min ^[1]

National Renewable Energy Lab. (NREL), Golden, CO (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

The lignocellulosic biomass is a promising renewable feedstock for biofuel production. Acetate is one of the major inhibitors liberated from hemicelluloses during hydrolysis. Likewise, an understanding of the toxic effects of acetate on the fermentation microorganism and the efficient utilization of mixed sugars of glucose and xylose in the presence of hydrolysate inhibitors is crucial for economic biofuel production.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1160173

Report Number(s):: NREL/JA-5100-61892; MainId:24294; UUID:f4a2d632-c3cb-e311-8d7c-d89d67132a6d; MainAdminID:12770

Journal Information:: Biotechnology for Biofuels, Vol. 7, Issue 9, September 2014; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 40 works

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References (48)

Inhibition of growth of Zymomonas mobilis by model compounds found in lignocellulosic hydrolysates Franden, Mary Ann; Pilath, Heidi M.; Mohagheghi, Ali Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-99	journal	January 2013
Effect of organic acids on the growth and fermentation of ethanologenicEscherichia coli LY01 Zaldivar, Jesus; Ingram, Lonnie O. Biotechnology and Bioengineering, Vol. 66, Issue 4 https://doi.org/10.1002/(SICI)1097-0290(1999)66:4<203::AID-BIT1>3.0.CO;2-#	journal	January 1999
Cellulosic hydrolysate toxicity and tolerance mechanisms in Escherichia coli Mills, Tirzah Y.; Sandoval, Nicholas R.; Gill, Ryan T. Biotechnology for Biofuels, Vol. 2, Issue 1, Article No. 26 https://doi.org/10.1186/1754-6834-2-26	journal	January 2009
Bacteria engineered for fuel ethanol production: current status Dien, B. S.; Cotta, M. A.; Jeffries, T. W. Applied Microbiology and Biotechnology, Vol. 63, Issue 3, p. 258-266 https://doi.org/10.1007/s00253-003-1444-y	journal	December 2003
Zymomonas mobilis: an alternative ethanol producer Panesar, Parmjit S.; Marwaha, Satwinder S.; Kennedy, John F. Journal of Chemical Technology & Biotechnology, Vol. 81, Issue 4 https://doi.org/10.1002/jctb.1448	journal	January 2006
The biology of Zymomonas. Swings, J.; De Ley, J. Bacteriological Reviews, Vol. 41, Issue 1 https://doi.org/10.1128/MMBR.41.1.1-46.1977	journal	January 1977
Draft Genome Sequence of the Flocculating Zymomonas mobilis Strain ZM401 (ATCC 31822) Zhao, N.; Bai, Y.; Zhao, X. -Q. Journal of Bacteriology, Vol. 194, Issue 24 https://doi.org/10.1128/JB.01947-12	journal	December 2012
Complete Genome Sequence of the Ethanol-Producing Zymomonas mobilis subsp. mobilis Centrotype ATCC 29191 Desiniotis, A.; Kouvelis, V. N.; Davenport, K. Journal of Bacteriology, Vol. 194, Issue 21 https://doi.org/10.1128/JB.01398-12	journal	October 2012
Genome Sequence of the Ethanol-Producing Zymomonas mobilis subsp. pomaceae Lectotype Strain ATCC 29192 Kouvelis, V. N.; Davenport, K. W.; Brettin, T. S. Journal of Bacteriology, Vol. 193, Issue 18 https://doi.org/10.1128/JB.05273-11	journal	July 2011
Complete Genome Sequence of the Ethanol Producer Zymomonas mobilis NCIMB 11163 Kouvelis, V. N.; Saunders, E.; Brettin, T. S. Journal of Bacteriology, Vol. 191, Issue 22 https://doi.org/10.1128/JB.01084-09	journal	September 2009
The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4 Seo, Jeong-Sun; Chong, Hyonyong; Park, Hyun Seok Nature Biotechnology, Vol. 23, Issue 1 https://doi.org/10.1038/nbt1045	journal	December 2004
Systems Biology Analysis of Zymomonas mobilis ZM4 Ethanol Stress Responses Yang, Shihui; Pan, Chongle; Tschaplinski, Timothy J. PLoS ONE, Vol. 8, Issue 7 https://doi.org/10.1371/journal.pone.0068886	journal	July 2013
The genome-scale metabolic network analysis of Zymomonas mobilis ZM4 explains physiological features and suggests ethanol and succinic acid production strategies Lee, Kyung; Park, Jong; Kim, Tae Microbial Cell Factories, Vol. 9, Issue 1 https://doi.org/10.1186/1475-2859-9-94	journal	January 2010
Genome-scale modeling and in silico analysis of ethanologenic bacteria Zymomonas mobilis Widiastuti, Hanifah; Kim, Jae Young; Selvarasu, Suresh Biotechnology and Bioengineering, Vol. 108, Issue 3 https://doi.org/10.1002/bit.22965	journal	November 2010
Biotechnological potential of respiring Zymomonas mobilis: A stoichiometric analysis of its central metabolism Pentjuss, Agris; Odzina, Ilona; Kostromins, Andrejs Journal of Biotechnology, Vol. 165, Issue 1 https://doi.org/10.1016/j.jbiotec.2013.02.014	journal	May 2013
Kinetic modeling to optimize pentose fermentation inZymomonas mobilis Altintas, Mehmet M.; Eddy, Christina K.; Zhang, Min Biotechnology and Bioengineering, Vol. 94, Issue 2 https://doi.org/10.1002/bit.20843	journal	January 2006
DNA restriction-modification systems in the ethanologen, Zymomonas mobilis ZM4 Kerr, Aidan L.; Jeon, Young Jae; Svenson, Charles J. Applied Microbiology and Biotechnology, Vol. 89, Issue 3 https://doi.org/10.1007/s00253-010-2936-1	journal	October 2010
Use of an EZ-Tn5-based random mutagenesis system to create a Zymomonas mobilis with significant tolerance to heat stress and malnutrition Jia, Xianghui; Wei, Na; Wang, Tianyv Journal of Industrial Microbiology & Biotechnology, Vol. 40, Issue 8 https://doi.org/10.1007/s10295-013-1287-1	journal	May 2013
Use of a Tn5-based transposon system to create a cost-effective Zymomonas mobilis for ethanol production from lignocelluloses Zhang, Xi; Wang, Tianyv; Zhou, Wen Microbial Cell Factories, Vol. 12, Issue 1 https://doi.org/10.1186/1475-2859-12-41	journal	January 2013
Dissecting a complex chemical stress: chemogenomic profiling of plant hydrolysates Skerker, Jeffrey M.; Leon, Dacia; Price, Morgan N. Molecular Systems Biology, Vol. 9, Issue 1 https://doi.org/10.1038/msb.2013.30	journal	January 2013
Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilis Zhang, M.; Eddy, C.; Deanda, K. Science, Vol. 267, Issue 5195 https://doi.org/10.1126/science.267.5195.240	journal	January 1995
Development of an arabinose-fermenting Zymomonas mobilis strain by metabolic pathway engineering. Deanda, K.; Zhang, M.; Eddy, C. Applied and environmental microbiology, Vol. 62, Issue 12 https://doi.org/10.1128/AEM.62.12.4465-4470.1996	journal	January 1996
Comparison of glucose/xylose co-fermentation by recombinant Zymomonas mobilis under different genetic and environmental conditions Ma, Yuanyuan; Dong, Huina; Zou, Shaolan Biotechnology Letters, Vol. 34, Issue 7 https://doi.org/10.1007/s10529-012-0897-4	journal	March 2012
Engineering efficient xylose metabolism into an acetic acid-tolerant Zymomonas mobilis strain by introducing adaptation-induced mutations Agrawal, Manoj; Wang, Yun; Chen, Rachel Ruizhen Biotechnology Letters, Vol. 34, Issue 10 https://doi.org/10.1007/s10529-012-0970-z	journal	June 2012
Characterization of Heterologous and Native Enzyme Activity Profiles in Metabolically Engineered Zymomonas mobilis Strains During Batch Fermentation of Glucose and Xylose Mixtures Gao, Qiang; Zhang, Min; McMillan, James D. Applied Biochemistry and Biotechnology, Vol. 98-100, Issue 1-9 https://doi.org/10.1385/ABAB:98-100:1-9:341	journal	January 2002
Over-expression of xylulokinase in a xylose-metabolising recombinant strain of Zymomonas mobilis Jae Jeon, Young; J. Svenson, Charles; L. Rogers, Peter FEMS Microbiology Letters, Vol. 244, Issue 1 https://doi.org/10.1016/j.femsle.2005.01.025	journal	March 2005
Cofermentation of Glucose, Xylose,and Arabinose by Genomic DNA-IntegratedXylose/Arabinose Fermenting Strainof <E1>Zymomonas mobilis</E1> AX101 Mohagheghi, Ali; Evans, Kent; Chou, Yat-Chen Applied Biochemistry and Biotechnology, Vol. 98-100, Issue 1-9 https://doi.org/10.1385/ABAB:98-100:1-9:885	journal	January 2002
Cofermentation of glucose, xylose, and arabinose by mixed cultures of two genetically engineeredZymomonas mobilis strains Mohagheghi, Ali; Evans, Kent; Finkelstein, Mark Applied Biochemistry and Biotechnology, Vol. 70-72, Issue 1 https://doi.org/10.1007/BF02920145	journal	March 1998
Transcriptome profiling of Zymomonas mobilis under ethanol stress He, Ming-xiong; Wu, Bo; Shui, Zong-xia Biotechnology for Biofuels, Vol. 5, Issue 1 https://doi.org/10.1186/1754-6834-5-75	journal	January 2012
Paradigm for industrial strain improvement identifies sodium acetate tolerance loci in Zymomonas mobilis and Saccharomyces cerevisiae Yang, S.; Land, M. L.; Klingeman, D. M. Proceedings of the National Academy of Sciences, Vol. 107, Issue 23 https://doi.org/10.1073/pnas.0914506107	journal	May 2010
An evolved xylose transporter from Zymomonas mobilis enhances sugar transport in Escherichia coli Ren, Chuan; Chen, Tingjian; Zhang, Jingqing Microbial Cell Factories, Vol. 8, Issue 1 https://doi.org/10.1186/1475-2859-8-66	journal	January 2009
Improved genome annotation for Zymomonas mobilis Yang, Shihui; Pappas, Katherine M.; Hauser, Loren J. Nature Biotechnology, Vol. 27, Issue 10 https://doi.org/10.1038/nbt1009-893	journal	October 2009
Transcriptomic and metabolomic profiling of Zymomonas mobilis during aerobic and anaerobic fermentations Yang, Shihui; Tschaplinski, Timothy J.; Engle, Nancy L. BMC Genomics, Vol. 10, Issue 1 https://doi.org/10.1186/1471-2164-10-34	journal	January 2009
STRING 8--a global view on proteins and their functional interactions in 630 organisms Jensen, L. J.; Kuhn, M.; Stark, M. Nucleic Acids Research, Vol. 37, Issue Database https://doi.org/10.1093/nar/gkn760	journal	January 2009
Transcriptome profiling of Zymomonas mobilis under furfural stress He, Ming-xiong; Wu, Bo; Shui, Zong-xia Applied Microbiology and Biotechnology, Vol. 95, Issue 1 https://doi.org/10.1007/s00253-012-4155-4	journal	May 2012
Genome-Wide Analysis of the General Stress Response Network in Escherichia coli: S-Dependent Genes, Promoters, and Sigma Factor Selectivity Weber, H.; Polen, T.; Heuveling, J. Journal of Bacteriology, Vol. 187, Issue 5 https://doi.org/10.1128/JB.187.5.1591-1603.2005	journal	February 2005
Metabolomic and transcriptomic stress response of Escherichia coli Jozefczuk, Szymon; Klie, Sebastian; Catchpole, Gareth Molecular Systems Biology, Vol. 6, Issue 1 https://doi.org/10.1038/msb.2010.18	journal	January 2010
Respiratory behaviour of a Zymomonas mobilis adhB::kan ^r mutant supports the hypothesis of two alcohol dehydrogenase isoenzymes catalysing opposite reactions Kalnenieks, U.; Galinina, N.; Toma, M. M. FEBS Letters, Vol. 580, Issue 21 https://doi.org/10.1016/j.febslet.2006.08.034	journal	August 2006
Discovery and characterization of a xylose reductase from Zymomonas mobilis ZM4 Agrawal, Manoj; Chen, Rachel Ruizhen Biotechnology Letters, Vol. 33, Issue 11 https://doi.org/10.1007/s10529-011-0677-6	journal	July 2011
Reduction of xylose to xylitol catalyzed by glucoseâ€“fructose oxidoreductase from Zymomonas mobilis Zhang, Xiaomei; Chen, Guanjun; Liu, Weifeng FEMS Microbiology Letters, Vol. 293, Issue 2 https://doi.org/10.1111/j.1574-6968.2009.01529.x	journal	April 2009
Clostridium thermocellum ATCC27405 transcriptomic, metabolomic and proteomic profiles after ethanol stress Yang, Shihui; Giannone, Richard J.; Dice, Lezlee BMC Genomics, Vol. 13, Issue 1 https://doi.org/10.1186/1471-2164-13-336	journal	January 2012
Generation of a proton motive force by histidine decarboxylation and electrogenic histidine/histamine antiport in Lactobacillus buchneri. Molenaar, D.; Bosscher, J. S.; ten Brink, B. Journal of Bacteriology, Vol. 175, Issue 10 https://doi.org/10.1128/jb.175.10.2864-2870.1993	journal	May 1993
Development of a high-throughput method to evaluate the impact of inhibitory compounds from lignocellulosic hydrolysates on the growth of Zymomonas mobilis Franden, Mary Ann; Pienkos, Philip T.; Zhang, Min Journal of Biotechnology, Vol. 144, Issue 4 https://doi.org/10.1016/j.jbiotec.2009.08.006	journal	December 2009
Improving xylose utilization by recombinant Zymomonas mobilis strain 8b through adaptation using 2-deoxyglucose Mohagheghi, Ali; Linger, Jeff; Smith, Holly Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/1754-6834-7-19	journal	January 2014
Cofermentation of Glucose, Xylose, and Arabinose by Mixed Cultures of Two Genetically Engineered Zymomonas mobilis Strains Mohagheghi, Ali; Evans, Kent; Finkelstein, Mark Biotechnology for Fuels and Chemicals https://doi.org/10.1007/978-1-4612-1814-2_28	book	January 1998
Ethanol cycle in an ethanologenic bacterium Kalnenieks, U.; Galinina, N.; Toma, M. M. FEBS Letters, Vol. 522, Issue 1-3 https://doi.org/10.1016/s0014-5793(02)02923-x	journal	June 2002
Identification and Inactivation of Genetic Loci Involved with Lactobacillus acidophilus Acid Tolerance Azcarate-Peril, M. A.; Altermann, E.; Hoover-Fitzula, R. L. Applied and Environmental Microbiology, Vol. 70, Issue 9 https://doi.org/10.1128/aem.70.9.5315-5322.2004	journal	September 2004
Elucidation of Zymomonas mobilis physiology and stress responses by quantitative proteomics and transcriptomics Yang, Shihui; Pan, Chongle; Hurst, Gregory B. Frontiers in Microbiology, Vol. 5 https://doi.org/10.3389/fmicb.2014.00246	journal	May 2014

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Improving a recombinant Zymomonas mobilis strain 8b through continuous adaptation on dilute acid pretreated corn stover hydrolysate Mohagheghi, Ali; Linger, Jeffrey G.; Yang, Shihui Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0233-z	journal	March 2015
Metabolic engineering of Zymomonas mobilis for 2,3-butanediol production from lignocellulosic biomass sugars Yang, Shihui; Mohagheghi, Ali; Franden, Mary Ann Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-016-0606-y	journal	September 2016
Complete genome sequence and the expression pattern of plasmids of the model ethanologen Zymomonas mobilis ZM4 and its xylose-utilizing derivatives 8b and 2032 Yang, Shihui; Vera, Jessica M.; Grass, Jeff Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1116-x	journal	May 2018
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09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
Zymomonas mobilis
acetate
xylose
transcriptomics
microarray
toxicity
pretreatment

Title: Insights into Acetate Toxicity in Zymomonas mobilis 8b using Different Substrates

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