TRANSCRIPTIONAL PROFILING USING THE FLOWTHROUGH GENOSENSOR FINAL CRADA REPORT C/ORNL97-00472

Technical Report · Tue Jun 01 00:00:00 EDT 1999

DOI:https://doi.org/10.2172/1149259· OSTI ID:1149259

Doktycz, M. J. ^[1]; Yang, H. ^[2]

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1. ORNL
2. Gene Logic Inc.

A Cooperative Research and Development Agreement (CRADA) between Lockheed Martin Energy Research Corporation (Contractor) and Gene Logic, Inc., (Participant) was carried out to evaluate the technical feasibility study of the application of the flowthrough genosensor for gene expression (transcriptional) profiling, over the current industry practice of using flat surface hybridization arrays to monitor the relative abundance of individual mRNA species in a cell. Various parameters, including substrate preparation, flow rates, hybridization conditions and sample concentrations, were evaluated on the flowthrough genosensor. The superiority of the flowthrough genosensor, in terms of hybridization rate and sensitivity were established.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: USDOE
• Contributing Organization: Gene Logic Inc.
• DOE Contract Number: DE-AC05-00OR22725
• OSTI ID: 1149259
• Report Number(s): C/ORNL97-0472; C/ORNL97-00472
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
genosensor
hybridization
cell