Real-time, single-step bioassay using nanoplasmonic resonator with ultra-high sensitivity

Zhang, Xiang; Ellman, Jonathan A; Chen, Fanqing Frank; Su, Kai-Hang; Wei, Qi-Huo; Sun, Cheng

Title: Real-time, single-step bioassay using nanoplasmonic resonator with ultra-high sensitivity

Patent · Tue Apr 01 00:00:00 EDT 2014

OSTI ID:1129019

Zhang, Xiang; Ellman, Jonathan A; Chen, Fanqing Frank; Su, Kai-Hang; Wei, Qi-Huo; Sun, Cheng

A nanoplasmonic resonator (NPR) comprising a metallic nanodisk with alternating shielding layer(s), having a tagged biomolecule conjugated or tethered to the surface of the nanoplasmonic resonator for highly sensitive measurement of enzymatic activity. NPRs enhance Raman signals in a highly reproducible manner, enabling fast detection of protease and enzyme activity, such as Prostate Specific Antigen (paPSA), in real-time, at picomolar sensitivity levels. Experiments on extracellular fluid (ECF) from paPSA-positive cells demonstrate specific detection in a complex bio-fluid background in real-time single-step detection in very small sample volumes.

View Patent

Cite

Export

Save

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC02-05CH11231

Assignee:: The Regents of the University of California (Oakland, CA)

Patent Number(s):: 8,685,743

Application Number:: 12/772,118

OSTI ID:: 1129019

Resource Relation:: Patent File Date: 2010 Apr 30

Country of Publication:: United States

Language:: English

References (41)

Raman-based system for DNA sequencing-mapping and other separations Vo-Dinh, Tuan https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5306403 US Patent Document 5,306,403	patent	April 1994
High resolution scanning raman microscope Quake, Stephen R. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6002471 US Patent Document 6,002,471	patent	December 1999
Advanced surface-enhanced Raman gene probe systems and methods thereof Vo-Dinh, Tuan https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6174677 US Patent Document 6,174,677	patent	January 2001
Resonant light scattering microparticle methods Prober, James M.; Cui, Xiumin; Dam, Rudy J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7122384 US Patent Document 7,122,384	patent	October 2006
Metal coated nanocrystalline silicon as an active surface enhanced raman spectroscopy (SERS) substrate Chan, Selena; Berlin, Andrew A.; Kwon, Sunghoon https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060215154 US Patent Application 11/264433; 20060215154	patent-application	September 2006
Surface plasmon-enhanced nano-optic devices and methods of making same Kim, Hong Koo; Sun, Zhijun; Jung, Yun Suk https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060273245 US Patent Application 10/566946; 20060273245	patent-application	December 2006
Elevated surface enhanced Raman spectroscopy resonator structures and method of making same Chen, Fanqing Frank; Chebi, Robert https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9857307 US Patent Document 9,857,307	patent	January 2018
Detection of protease and protease activity using a single nanocrescent SERS probe Liu, Gang; Ellman, Jonathan A.; Lee, Luke P. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9145575 US Patent Document 9,145,575	patent	September 2015
Tunable and augmented plasmon resonances of Au∕SiO2∕Au nanodisks Su, K. H.; Wei, Q. H.; Zhang, X. Applied Physics Letters, Vol. 88, Issue 6 https://doi.org/10.1063/1.2172712	journal	February 2006
Modulation of the enzymatic activity of papain by interdomain residues remote from the active site Altschuh, Danièle; Tessier, Daniel C.; Vernet, Thierry "Protein Engineering, Design and Selection", Vol. 7, Issue 6 https://doi.org/10.1093/protein/7.6.769	journal	January 1994
Molecular diagnosis of prostate cancer Canto, Eduardo I.; Shariat, Shahrokh F.; Slawin, Kevin M. Current Urology Reports, Vol. 5, Issue 3 https://doi.org/10.1007/s11934-004-0038-2	journal	May 2004
Raman Dye-Labeled Nanoparticle Probes for Proteins Cao, Y. Charles; Jin, Rongchao; Nam, Jwa-Min Journal of the American Chemical Society, Vol. 125, Issue 48 https://doi.org/10.1021/ja0366235	journal	December 2003
Nanoparticles with Raman Spectroscopic Fingerprints for DNA and RNA Detection Cao, Y. C. Science, Vol. 297, Issue 5586 https://doi.org/10.1126/science.297.5586.1536	journal	August 2002
Prostate-Specific Antigen and the Early Diagnosis of Prostate Cancer Caplan, Aaron; Kratz, Alexander Pathology Patterns Reviews, Vol. 117, Issue suppl_1 https://doi.org/10.1309/C4UN-12LK-43HP-JXY3	journal	June 2002
The Tissue Kallikrein Family of Serine Proteases: Functional Roles in Human Disease and Potential as Clinical Biomarkers Clements, Judith A.; Willemsen, Nicole M.; Myers, Stephen A. Critical Reviews in Clinical Laboratory Sciences, Vol. 41, Issue 3 https://doi.org/10.1080/10408360490471931	journal	January 2004
The role of prostate-specific antigen in the clinical evaluation of prostatic disease Denmeade, S. R.; Isaacs, J. T. BJU International, Vol. 93, Issue s1 https://doi.org/10.1111/j.1464-410X.2003.04634.x	journal	March 2004
A history of prostate cancer treatment Denmeade, Samuel R.; Isaacs, John T. Nature Reviews Cancer, Vol. 2, Issue 5 https://doi.org/10.1038/nrc801	journal	May 2002
Prostate-Specific Antigen-Activated Thapsigargin Prodrug as Targeted Therapy for Prostate Cancer Denmeade, S. R.; Jakobsen, C. M.; Janssen, S. JNCI Journal of the National Cancer Institute, Vol. 95, Issue 13 https://doi.org/10.1093/jnci/95.13.990	journal	July 2003
Self-Assembled Metal Colloid Monolayers: An Approach to SERS Substrates Freeman, R. G.; Grabar, K. C.; Allison, K. J. Science, Vol. 267, Issue 5204 https://doi.org/10.1126/science.267.5204.1629	journal	March 1995
PSA markers in prostate cancer detection Gretzer, Matthew B.; Partin, Alan W. Urologic Clinics of North America, Vol. 30, Issue 4 https://doi.org/10.1016/S0094-0143(03)00057-0	journal	November 2003
Prostate cancer epidemiology Grönberg, Henrik The Lancet, Vol. 361, Issue 9360 https://doi.org/10.1016/S0140-6736(03)12713-4	journal	March 2003
New biochip technology for label-free detection of pathogens and their toxins Grow, Ann E.; Wood, Laurie L.; Claycomb, Johanna L. Journal of Microbiological Methods, Vol. 53, Issue 2, p. 221-233 https://doi.org/10.1016/S0167-7012(03)00026-5	journal	May 2003
Prostate-specific antigen and related isoforms in the diagnosis and management of prostate cancer Haese, Alexander; Graefen, Markus; Huland, Hartwig Current Urology Reports, Vol. 5, Issue 3 https://doi.org/10.1007/s11934-004-0042-6	journal	May 2004
Plasmon-Sampled Surface-Enhanced Raman Excitation Spectroscopy ^† Haynes, Christy L.; Van Duyne, Richard P. The Journal of Physical Chemistry B, Vol. 107, Issue 30 https://doi.org/10.1021/jp027749b	journal	July 2003
Surface-enhanced Raman scattering on tunable plasmonic nanoparticle substrates Jackson, J. B.; Halas, N. J. Proceedings of the National Academy of Sciences, Vol. 101, Issue 52, p. 17930-17935 https://doi.org/10.1073/pnas.0408319102	journal	December 2004
Periodically structured metallic substrates for SERS Kahl, M.; Voges, E.; Kostrewa, S. Sensors and Actuators B: Chemical, Vol. 51, Issue 1-3, p. 285-291 https://doi.org/10.1016/S0925-4005(98)00219-6	journal	August 1998
Anthrax toxin lethal factor contains a zinc metalloprotease consensus sequence which is required for lethal toxin activity Klimpel, Kurt R.; Arora, Naveen; Leppla, Stephen H. Molecular Microbiology, Vol. 13, Issue 6 https://doi.org/10.1111/j.1365-2958.1994.tb00500.x	journal	September 1994
Real-Time Glucose Sensing by Surface-Enhanced Raman Spectroscopy in Bovine Plasma Facilitated by a Mixed Decanethiol/Mercaptohexanol Partition Layer Lyandres, Olga; Shah, Nilam C.; Yonzon, Chanda Ranjit Analytical Chemistry, Vol. 77, Issue 19 https://doi.org/10.1021/ac051357u	journal	October 2005
Surface-enhanced spectroscopy Moskovits, Martin Reviews of Modern Physics, Vol. 57, Issue 3 https://doi.org/10.1103/RevModPhys.57.783	journal	July 1985
Probing Single Molecules and Single Nanoparticles by Surface-Enhanced Raman Scattering Nie, Shuming; Emory, Steven R. Science, Vol. 275, Issue 5303, p. 1102-1106 https://doi.org/10.1126/science.275.5303.1102	journal	February 1997
Characterization and application of Raman labels for confocal Raman microspectroscopic detection of cellular proteins in single cells Nithipatikom, Kasem; McCoy, Michael J.; Hawi, Sharon R. Analytical Biochemistry, Vol. 322, Issue 2, p. 198-207 https://doi.org/10.1016/j.ab.2003.07.020	journal	November 2003
Catalytic hydroxyl/amine dyads within serine proteases Paetzel, Mark; Dalbey, Ross E. Trends in Biochemical Sciences, Vol. 22, Issue 1, p. 28-31 https://doi.org/10.1016/S0968-0004(96)10065-7	journal	January 1997
On the size of the active site in proteases. I. Papain Schechter, Israel; Berger, Arieh Biochemical and Biophysical Research Communications, Vol. 27, Issue 2, p. 157-162 https://doi.org/10.1016/S0006-291X(67)80055-X	journal	April 1967
The three-dimensional structure of the aspartyl protease from the HIV-1 isolate BRU Spinelli, S.; Liu, Q.; Alzari, P. Biochimie, Vol. 73, Issue 11 https://doi.org/10.1016/0300-9084(91)90169-2	journal	November 1991
Surface-enhanced Raman spectroscopy of half-mustard agent Stuart, Douglas A.; Biggs, Kevin B.; Van Duyne, Richard P. The Analyst, Vol. 131, Issue 4 https://doi.org/10.1039/b513326b	journal	January 2006
Raman Enhancement Factor of a Single Tunable Nanoplasmonic Resonator Su, Kai-Hung; Durant, Stéphane; Steele, Jennifer M. The Journal of Physical Chemistry B, Vol. 110, Issue 9 https://doi.org/10.1021/jp055566u	journal	March 2006
Surface-Enhanced Raman Detection of 2,4-Dinitrotoluene Impurity Vapor as a Marker To Locate Landmines Sylvia, James M.; Janni, James A.; Klein, J. D. Analytical Chemistry, Vol. 72, Issue 23 https://doi.org/10.1021/ac0006573	journal	December 2000
Surface-enhanced Raman scattering for medical diagnostics and biological imaging Vo-Dinh, Tuan; Yan, Fei; Wabuyele, Musundi B. Journal of Raman Spectroscopy, Vol. 36, Issue 6-7 https://doi.org/10.1002/jrs.1348	journal	January 2005
Immunopeptidometric Assay for Enzymatically Active Prostate-Specific Antigen Wu, P. Clinical Chemistry, Vol. 50, Issue 1 https://doi.org/10.1373/clinchem.2003.026146	journal	January 2004
Separation of enzymatically active and inactive prostate-specific antigen (PSA) by peptide affinity chromatography: PSA-Specific Peptides Wu, Ping; Stenman, Ulf-Håkan; Pakkala, Miikka The Prostate, Vol. 58, Issue 4 https://doi.org/10.1002/pros.10337	journal	November 2003
A Glucose Biosensor Based on Surface-Enhanced Raman Scattering: Improved Partition Layer, Temporal Stability, Reversibility, and Resistance to Serum Protein Interference Yonzon, Chanda Ranjit; Haynes, Christy L.; Zhang, Xiaoyu Analytical Chemistry, Vol. 76, Issue 1 https://doi.org/10.1021/ac035134k	journal	January 2004

Similar Records

Nanoplasmonic molecular ruler for nuclease activity and DNAfootprinting

Journal Article · Tue Aug 15 00:00:00 EDT 2006 · Nature Nanotechnology · OSTI ID:1129019

Chen, Fanqing Frank; Liu, Gang L; Yin, Yadong; +7 more

Membrane cholesterol modulates STEAP2 conformation during dynamic intracellular trafficking processes leading to broad subcellular distribution

Journal Article · Sat Sep 15 00:00:00 EDT 2018 · Experimental Cell Research · OSTI ID:1129019

Hasegawa, Haruki; Li, Cong; Alba, Benjamin M.; +3 more

Nanoplasmonic molecular ruler for nuclease activity and DNA footprinting

Patent · Tue Oct 29 00:00:00 EDT 2013 · OSTI ID:1129019

Chen, Fanqing Frank; Liu, Gang L; Lee, Luke P

Related Subjects

47 OTHER INSTRUMENTATION

Title: Real-time, single-step bioassay using nanoplasmonic resonator with ultra-high sensitivity

Citation Formats

References (41)

Similar Records

Related Subjects