Report for Evaluation of Canonical SNP Taqman Assays to Detect Biothreat Agents and Environmental Samples for DHS
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
The objective of this project is to provide DHS a comprehensive evaluation of the current genomic technologies including genotyping, Taqman PCR, multiple locus variable tandem repeat analysis (MLVA), microarray and high-throughput DNA sequencing in the analysis of biothreat agents from complex environmental samples. Taqman assays were designed to detect the canonical SNPs to discriminate different strains of Bacillus anthracis. The assays were run to test purified DNA from several strains of B. anthracis and BioWatch aerosol filter extracts and soil samples that were spiked with B. anthracis. The assays were very sensitive. We were able to detect signatures specific to B. anthracis Ames at the 10-100 genome copy level after spiking B. anthracis Ames DNA into BioWatch aerosol filter extracts 100-1000 when spiked into soil samples.
- Research Organization:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 1122226
- Report Number(s):
- LLNL-TR-482763
- Country of Publication:
- United States
- Language:
- English
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