Method of detecting genetic deletions identified with chromosomal abnormalities

Gray, Joe W; Pinkel, Daniel; Tkachuk, Douglas

Title: Method of detecting genetic deletions identified with chromosomal abnormalities

Patent · Tue Nov 26 00:00:00 EST 2013

OSTI ID:1109099

Gray, Joe W; Pinkel, Daniel; Tkachuk, Douglas

Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyzes. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acids probes are typically of a complexity greater tha 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particlularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML) and for biological dosimetry. Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar ut genetically different diseases, and for many prognostic and diagnostic applications.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: W-7405-ENG-48

Assignee:: The Regents of the University of California (Oakland, CA)

Patent Number(s):: 8,592,155

Application Number:: 10/608,092

OSTI ID:: 1109099

Country of Publication:: United States

Language:: English

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