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Title: Nanoplasmonic molecular ruler for nuclease activity and DNA footprinting

Abstract

This invention provides a nanoplasmonic molecular ruler, which can perform label-free and real-time monitoring of nucleic acid (e.g., DNA) length changes and perform nucleic acid footprinting. In various embodiments the ruler comprises a nucleic acid attached to a nanoparticle, such that changes in the nucleic acid length are detectable using surface plasmon resonance. The nanoplasmonic ruler provides a fast and convenient platform for mapping nucleic acid-protein interactions, for nuclease activity monitoring, and for other footprinting related methods.

Inventors:
; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1107635
Patent Number(s):
8,569,468
Application Number:
12/440,579
Assignee:
The Regents of the University of California (Oakland, CA)
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Chen, Fanqing Frank, Liu, Gang L, and Lee, Luke P. Nanoplasmonic molecular ruler for nuclease activity and DNA footprinting. United States: N. p., 2013. Web.
Chen, Fanqing Frank, Liu, Gang L, & Lee, Luke P. Nanoplasmonic molecular ruler for nuclease activity and DNA footprinting. United States.
Chen, Fanqing Frank, Liu, Gang L, and Lee, Luke P. 2013. "Nanoplasmonic molecular ruler for nuclease activity and DNA footprinting". United States. https://www.osti.gov/servlets/purl/1107635.
@article{osti_1107635,
title = {Nanoplasmonic molecular ruler for nuclease activity and DNA footprinting},
author = {Chen, Fanqing Frank and Liu, Gang L and Lee, Luke P},
abstractNote = {This invention provides a nanoplasmonic molecular ruler, which can perform label-free and real-time monitoring of nucleic acid (e.g., DNA) length changes and perform nucleic acid footprinting. In various embodiments the ruler comprises a nucleic acid attached to a nanoparticle, such that changes in the nucleic acid length are detectable using surface plasmon resonance. The nanoplasmonic ruler provides a fast and convenient platform for mapping nucleic acid-protein interactions, for nuclease activity monitoring, and for other footprinting related methods.},
doi = {},
url = {https://www.osti.gov/biblio/1107635}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Oct 29 00:00:00 EDT 2013},
month = {Tue Oct 29 00:00:00 EDT 2013}
}

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