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Title: Method for in vitro recombination

Patent ·
OSTI ID:1084234
;

The present invention relates to an in vitro method, using isolated protein reagents, for joining two double-stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest.

Research Organization:
Synthetic Genomics, Inc., La Jolla, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG02-02ER63453
Assignee:
Synthetic Genomics, Inc. (La Jolla, CA)
Patent Number(s):
8,435,736
Application Number:
12/820,861
OSTI ID:
1084234
Resource Relation:
Patent File Date: 2010 Jun 22
Country of Publication:
United States
Language:
English

References (8)

A multifunctional exonuclease from vegetative Schizosaccharomyces pombe cells exhibiting in vitro strand exchange activity. journal May 1994
Macromolecular crowding increases binding of DNA polymerase to DNA: an adaptive effect. journal April 1987
Method for the complete chemical synthesis and assembly of genes and genomes patent February 2003
In Vitro Recombination Method patent-application February 2007
Macromolecular crowding accelerates the cohesion of DNA fragments with complementary termini journal January 1985
Evolution of whole cells and organisms by recursive sequence recombination patent April 2002
Stimulation of enzymatic ligation of DNA by high concentrations of nonspecific polymers patent April 1986
Repair of double-strand breaks by incorporation of a molecule of homologous DNA journal April 2000

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