Portable dual field gradient force multichannel flow cytometer device with a dual wavelength low noise detection scheme

James, Conrad D; Galambos, Paul C; Derzon, Mark S; Graf, Darin C; Pohl, Kenneth R; Bourdon, Chris J

Title: Portable dual field gradient force multichannel flow cytometer device with a dual wavelength low noise detection scheme

Patent · Tue Oct 23 00:00:00 EDT 2012

OSTI ID:1079398

James, Conrad D; Galambos, Paul C; Derzon, Mark S; Graf, Darin C; Pohl, Kenneth R; Bourdon, Chris J

Systems and methods for combining dielectrophoresis, magnetic forces, and hydrodynamic forces to manipulate particles in channels formed on top of an electrode substrate are discussed. A magnet placed in contact under the electrode substrate while particles are flowing within the channel above the electrode substrate allows these three forces to be balanced when the system is in operation. An optical detection scheme using near-confocal microscopy for simultaneously detecting two wavelengths of light emitted from the flowing particles is also discussed.

View Patent

Cite

Export

Save

Research Organization:: Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC04-94AL85000

Assignee:: Sandia Corporation (Albuquerque, NM)

Patent Number(s):: 8,293,089

Application Number:: 12/610,409

OSTI ID:: 1079398

Country of Publication:: United States

Language:: English

References (26)

Automated methods for multiplexed pathogen detection Straub, Timothy M.; Dockendorff, Brian P.; Quiñonez-Díaz, Maria D. Journal of Microbiological Methods, Vol. 62, Issue 3 https://doi.org/10.1016/j.mimet.2005.04.012	journal	September 2005
Biofunctional magnetic nanoparticles for protein separation and pathogen detection Gu, Hongwei; Xu, Keming; Xu, Chenjie Chemical Communications, Issue 9 https://doi.org/10.1039/b514130c	journal	January 2006
Timely multithreat biological, chemical and nuclide detection: a platform, a metric, key results Derzon, Mark S.; Hopkins, Matthew M.; Galambos, Paul C. International Journal of Technology Transfer and Commercialisation, Vol. 7, Issue 4 https://doi.org/10.1504/IJTTC.2008.021038	journal	January 2008
Continuous sorting of magnetic cells via on-chip free-flow magnetophoresis Pamme, Nicole; Wilhelm, Claire Lab on a Chip, Vol. 6, Issue 8 https://doi.org/10.1039/b604542a	journal	January 2006
Encapsulation of organic field effect transistors for flexible biomedical microimplants Feili, Dara; Schuettler, Martin; Doerge, Thomas Sensors and Actuators A: Physical, Vol. 120, Issue 1 https://doi.org/10.1016/j.sna.2004.11.021	journal	April 2005
Combined field-induced dielectrophoresis and phase separation for manipulating particles in microfluidics Bennett, Dawn J.; Khusid, Boris; James, Conrad D. Applied Physics Letters, Vol. 83, Issue 23 https://doi.org/10.1063/1.1629789	journal	December 2003
3D focusing of nanoparticles in microfluidic channels Morgan, H.; Morgan, H.; Holmes, D. IEE Proceedings - Nanobiotechnology, Vol. 150, Issue 2 https://doi.org/10.1049/ip-nbt:20031090	journal	January 2003
Magnetic bead handling on-chip: new opportunities for analytical applications Gijs, Martin A. M. Microfluidics and Nanofluidics https://doi.org/10.1007/s10404-004-0010-y	journal	October 2004
Vertical Focusing Device Utilizing Dielectrophoretic Force and Its Application on Microflow Cytometer Lin, C. -H.; Lee, G. -B.; Fu, L. -M. Journal of Microelectromechanical Systems, Vol. 13, Issue 6 https://doi.org/10.1109/JMEMS.2004.838352	journal	December 2004
Analyzing a bioterror attack on the food supply: The case of botulinum toxin in milk Wein, L. M.; Liu, Y. Proceedings of the National Academy of Sciences, Vol. 102, Issue 28 https://doi.org/10.1073/pnas.0408526102	journal	June 2005
Immunomagnetic detection of Bacillus stearothermophilus spores in food and environmental samples. Blake, M. R.; Weimer, B. C. Applied and environmental microbiology, Vol. 63, Issue 5 https://doi.org/10.1128/AEM.63.5.1643-1646.1997	journal	January 1997
Fast, sensitive and cost-effective detection of nerve agents in the gas phase using a portable instrument and an electrochemical biosensor Arduini, Fabiana; Amine, Aziz; Moscone, Danila Analytical and Bioanalytical Chemistry, Vol. 388, Issue 5-6 https://doi.org/10.1007/s00216-007-1330-z	journal	May 2007
High throughput particle analysis: Combining dielectrophoretic particle focussing with confocal optical detection Holmes, David; Morgan, Hywel; Green, Nicolas G. Biosensors and Bioelectronics, Vol. 21, Issue 8, p. 1621-1630 https://doi.org/10.1016/j.bios.2005.10.017	journal	February 2006
Recent advancements in the gas-phase MicroChemLab Le, P. R.; Manginell, P.; Adkins, D. R. IEEE Sensors Journal, Vol. 6, Issue 3 https://doi.org/10.1109/JSEN.2006.874495	journal	June 2006
Tungsten coil atomic emission spectrometry Rust, Jennifer A.; Nóbrega, Joaquim A.; Calloway, Clifton P. Spectrochimica Acta Part B: Atomic Spectroscopy, Vol. 61, Issue 2 https://doi.org/10.1016/j.sab.2005.12.009	journal	February 2006
Multiplexed Liquid Arrays for Simultaneous Detection of Simulants of Biological Warfare Agents McBride, Mary T.; Gammon, Stuart; Pitesky, Maurice Analytical Chemistry, Vol. 75, Issue 8 https://doi.org/10.1021/ac026379k	journal	April 2003
Monolithic surface micromachined fluidic devices for dielectrophoretic preconcentration and routing of particles James, Conrad D.; Okandan, Murat; Mani, Seethambal S. Journal of Micromechanics and Microengineering, Vol. 16, Issue 10 https://doi.org/10.1088/0960-1317/16/10/001	journal	August 2006
A three-dimensional dielectrophoretic particle focusing channel for microcytometry applications Yu, Choongho; Vykoukal, J.; Vykoukal, D. M. Journal of Microelectromechanical Systems, Vol. 14, Issue 3, p. 480-487 https://doi.org/10.1109/JMEMS.2005.844839	journal	June 2005
Portable remote Raman system for monitoring hydrocarbon, gas hydrates and explosives in the environment Sharma, Shiv K.; Misra, Anupam K.; Sharma, Bhavna Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Vol. 61, Issue 10 https://doi.org/10.1016/j.saa.2005.02.020	journal	August 2005
Enzymatically-generated fluorescent detection in micro-channels with internal magnetic mixing for the development of parallel microfluidic ELISA Herrmann, M.; Veres, T.; Tabrizian, M. Lab on a Chip, Vol. 6, Issue 4 https://doi.org/10.1039/b516031f	journal	January 2006
Automated immunomagnetic separation and microarray detection of E. coli O157:H7 from poultry carcass rinse Chandler, D. International Journal of Food Microbiology, Vol. 70, Issue 1-2 https://doi.org/10.1016/S0168-1605(01)00536-0	journal	October 2001
Rapid, femtomolar bioassays in complex matrices combining microfluidics and magnetoelectronics Mulvaney, S. P.; Cole, C. L.; Kniller, M. D. Biosensors and Bioelectronics, Vol. 23, Issue 2 https://doi.org/10.1016/j.bios.2007.03.029	journal	September 2007
Portable 24-analyte surface plasmon resonance instruments for rapid, versatile biodetection Chinowsky, Timothy M.; Soelberg, Scott D.; Baker, Paul Biosensors and Bioelectronics, Vol. 22, Issue 9-10 https://doi.org/10.1016/j.bios.2006.11.026	journal	April 2007
Classification of Chemical and Biological Warfare Agent Simulants by Surface-Enhanced Raman Spectroscopy and Multivariate Statistical Techniques Pearman, William F.; Fountain, Augustus W. Applied Spectroscopy, Vol. 60, Issue 4 https://doi.org/10.1366/000370206776593744	journal	April 2006
Binary electrokinetic separation of target DNA from background DNA primers. James, Conrad; Derzon, Mark https://doi.org/10.2172/876396	report	October 2005
Microfluidic ELISA on non-passivated PDMS chip using magnetic bead transfer inside dual networks of channels Herrmann, Marc; Roy, Emmanuel; Veres, Teodor Lab on a Chip, Vol. 7, Issue 11 https://doi.org/10.1039/b707883h	journal	January 2007

Similar Records

Manipulation of DNA for use in microfluidic devices

Conference · Wed Nov 18 00:00:00 EST 1998 · OSTI ID:1079398

Belgrader, P; Bettencourt, K; Davidson, J C; +4 more

Flow cytometer for resolving signals from heterogeneous fluorescence emissions and quantifying lifetime in fluorochrome-labeled cells/particles by phase-sensitive detection

Journal Article · Wed Dec 01 00:00:00 EST 1993 · Review of Scientific Instruments; (United States) · OSTI ID:1079398

Steinkamp, J A; Yoshida, T M; Martin, J C

AC electrokinetic manipulation of selenium nanoparticles for potential nanosensor applications

Journal Article · Fri Mar 15 00:00:00 EDT 2013 · Materials Research Bulletin · OSTI ID:1079398

Mahmoodi, Seyed Reza; Bayati, Marzieh; Hosseinirad, Somayeh; +3 more

Related Subjects

47 OTHER INSTRUMENTATION

Title: Portable dual field gradient force multichannel flow cytometer device with a dual wavelength low noise detection scheme

Citation Formats

References (26)

Similar Records

Related Subjects