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Title: Effects of remediation amendments on vadose zone microorganisms

Abstract

Surfactant-based foam delivery technology has been studied to remediate Hanford 200 area deep vadose zone sediment. However, the surfactants and remediation amendments have an unknown effect on indigenous subsurface microorganisms. Microbial populations are important factors to consider in remediation efforts due to their potential to alter soil geochemistry. This project focuses on measuring microbial metabolic responses to remediation amendments in batch and column studies using Deep Vadose Zone Sediments. Initial studies of the microbes from Hanford 200 area deep vadose zone sediment showed surfactants sodium dodecyl sulfate (SDS) and cocamidopropyl betaine (CAPB) and remediation amendment calcium polysulfide (CPS) had no affect on microbial growth using BiologTM Ecoplates. To move towards a more realistic field analog, soil columns were packed with Hanford 200 Area sediment. Once microbial growth in the column was verified by observing growth of the effluent solution on tryptic soy agar plates, remedial surfactants were injected into the columns, and the resulting metabolic diversity was measured. Results suggest surfactant sodium dodecyl sulfate (SDS) stimulates microbial growth. The soil columns were also visualized using X-ray microtomography to inspect soil packing and possibly probe for evidence of biofilms. Overall, BiologTM Ecoplates provide a rapid assay to predict effects of remediationmore » amendments on Hanford 200 area deep vadose zone microorganisms.« less

Authors:
;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1052525
Report Number(s):
PNNL-21611
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English

Citation Formats

Miller, Hannah M, and Tilton, Fred A. Effects of remediation amendments on vadose zone microorganisms. United States: N. p., 2012. Web. doi:10.2172/1052525.
Miller, Hannah M, & Tilton, Fred A. Effects of remediation amendments on vadose zone microorganisms. United States. https://doi.org/10.2172/1052525
Miller, Hannah M, and Tilton, Fred A. 2012. "Effects of remediation amendments on vadose zone microorganisms". United States. https://doi.org/10.2172/1052525. https://www.osti.gov/servlets/purl/1052525.
@article{osti_1052525,
title = {Effects of remediation amendments on vadose zone microorganisms},
author = {Miller, Hannah M and Tilton, Fred A},
abstractNote = {Surfactant-based foam delivery technology has been studied to remediate Hanford 200 area deep vadose zone sediment. However, the surfactants and remediation amendments have an unknown effect on indigenous subsurface microorganisms. Microbial populations are important factors to consider in remediation efforts due to their potential to alter soil geochemistry. This project focuses on measuring microbial metabolic responses to remediation amendments in batch and column studies using Deep Vadose Zone Sediments. Initial studies of the microbes from Hanford 200 area deep vadose zone sediment showed surfactants sodium dodecyl sulfate (SDS) and cocamidopropyl betaine (CAPB) and remediation amendment calcium polysulfide (CPS) had no affect on microbial growth using BiologTM Ecoplates. To move towards a more realistic field analog, soil columns were packed with Hanford 200 Area sediment. Once microbial growth in the column was verified by observing growth of the effluent solution on tryptic soy agar plates, remedial surfactants were injected into the columns, and the resulting metabolic diversity was measured. Results suggest surfactant sodium dodecyl sulfate (SDS) stimulates microbial growth. The soil columns were also visualized using X-ray microtomography to inspect soil packing and possibly probe for evidence of biofilms. Overall, BiologTM Ecoplates provide a rapid assay to predict effects of remediation amendments on Hanford 200 area deep vadose zone microorganisms.},
doi = {10.2172/1052525},
url = {https://www.osti.gov/biblio/1052525}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Aug 10 00:00:00 EDT 2012},
month = {Fri Aug 10 00:00:00 EDT 2012}
}