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Title: Antifungal polypeptides

Patent ·
OSTI ID:1041036

Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC02-05CH11231
Assignee:
Pioneer Hi-Bred International, Inc. (Johnston, IA); E. I. du Pont de Nemours and Company (Wilmington, DE); The Regents of the University of California (Oakland, CA)
Patent Number(s):
8,148,510
Application Number:
12/795,770
OSTI ID:
1041036
Country of Publication:
United States
Language:
English

References (6)

Purification, Characterisation and cDNA Cloning of an Antimicrobial Peptide from Macadamia Integrifolia journal March 1997
Functional Analysis of Conserved Histidines in ADP-Glucose Pyrophosphorylase from Escherichia coli journal March 1998
Transforming growth factor alpha: mutation of aspartic acid 47 and leucine 48 results in different biological activities. journal March 1988
Isolation and Characterization of a Novel Antifungal Peptide from Aspergillus niger journal October 1999
In Defense against Pathogens. Both Plant Sentinels and Foot Soldiers Need to Know the Enemy: Fig. 1. journal April 2003
Functional Domain Analysis of the Yeast ABC Transporter Ycf1p by Site-directed Mutagenesis journal August 1999

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