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Title: Methods and compositions for the production of orthogonal tRNA-aminoacyl-tRNA synthetase pairs

Abstract

This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.

Inventors:
 [1];  [2];  [2];  [2];  [3];  [4];  [5];  [2];  [2];  [2];  [2]
  1. La Jolla, CA
  2. San Diego, CA
  3. Lexington, MA
  4. North Haven, CT
  5. Philadelphia, PA
Publication Date:
Research Org.:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1027459
Patent Number(s):
8,012,739
Application Number:
11/978,154
Assignee:
The Scripps Research Institute (La Jolla, CA); The Regents of the University of California (Oakland, CA)
DOE Contract Number:  
FG03-00ER45812; AC03-76SF00098
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Schultz, Peter G, Wang, Lei, Anderson, John Christopher, Chin, Jason W, Liu, David R, Magliery, Thomas J, Meggers, Eric L, Mehl, Ryan Aaron, Pastrnak, Miro, Santoro, Stephen William, and Zhang, Zhiwen. Methods and compositions for the production of orthogonal tRNA-aminoacyl-tRNA synthetase pairs. United States: N. p., 2011. Web.
Schultz, Peter G, Wang, Lei, Anderson, John Christopher, Chin, Jason W, Liu, David R, Magliery, Thomas J, Meggers, Eric L, Mehl, Ryan Aaron, Pastrnak, Miro, Santoro, Stephen William, & Zhang, Zhiwen. Methods and compositions for the production of orthogonal tRNA-aminoacyl-tRNA synthetase pairs. United States.
Schultz, Peter G, Wang, Lei, Anderson, John Christopher, Chin, Jason W, Liu, David R, Magliery, Thomas J, Meggers, Eric L, Mehl, Ryan Aaron, Pastrnak, Miro, Santoro, Stephen William, and Zhang, Zhiwen. 2011. "Methods and compositions for the production of orthogonal tRNA-aminoacyl-tRNA synthetase pairs". United States. https://www.osti.gov/servlets/purl/1027459.
@article{osti_1027459,
title = {Methods and compositions for the production of orthogonal tRNA-aminoacyl-tRNA synthetase pairs},
author = {Schultz, Peter G and Wang, Lei and Anderson, John Christopher and Chin, Jason W and Liu, David R and Magliery, Thomas J and Meggers, Eric L and Mehl, Ryan Aaron and Pastrnak, Miro and Santoro, Stephen William and Zhang, Zhiwen},
abstractNote = {This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.},
doi = {},
url = {https://www.osti.gov/biblio/1027459}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Sep 06 00:00:00 EDT 2011},
month = {Tue Sep 06 00:00:00 EDT 2011}
}

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