High speed nucleic acid sequencing

Korlach, Jonas; Webb, Watt W; Levene, Michael; Turner, Stephen; Craighead, Harold G; Foquet, Mathieu

Title: High speed nucleic acid sequencing

Patent · Tue May 17 00:00:00 EDT 2011

OSTI ID:1020256

Korlach, Jonas ^[1]; Webb, Watt W ^[1]; Levene, Michael ^[1]; Turner, Stephen ^[1]; Craighead, Harold G ^[1]; Foquet, Mathieu ^[1]

Ithaca, NY

The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid. Each type of labeled nucleotide comprises an acceptor fluorophore attached to a phosphate portion of the nucleotide such that the fluorophore is removed upon incorporation into a growing strand. Fluorescent signal is emitted via fluorescent resonance energy transfer between the donor fluorophore and the acceptor fluorophore as each nucleotide is incorporated into the growing strand. The sequence is deduced by identifying which base is being incorporated into the growing strand.

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Research Organization:: Cornell Research Foundation (Ithaca, NY)

Sponsoring Organization:: USDOE

DOE Contract Number:: 066898-0003891

Assignee:: Cornell Research Foundation (Ithaca, NY)

Patent Number(s):: 7,943,305

Application Number:: 11/279,711

OSTI ID:: 1020256

Country of Publication:: United States

Language:: English

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