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Title: Effects of freezing and cold acclimation on the plasma membrane of isolated protoplasts. Summary progress report, May 16, 1987--June 1, 1991

Abstract

This project focuses on lesions in the plasma membrane of protoplasts that occur during freezing to temperatures below {minus}5{degrees} which result in changes in the semipermeablity of the plasma membrane. This injury, referred to as loss of osmotic responsiveness, is associated with the formation of large, aparticulate domains in the plasma membrane, aparticulate lamellae subtending the plasma membrane, and lamellar-to-hexagonal{sub II} phase transitions in the plasma membrane and subtending lamellar. The goals of this project are to provide a mechanistic understanding of the mechanism by which freeze-induced dehydration effects the formation of aparticulate domains and lamellar-to-hexagonal{sub II} phase transitions and to determine the mechanisms by which cold acclimation and cryoprotectants preclude or diminish these ultrastructural changes. Our working hypothesis is the formation of aparticulate domains and lamellar-to-hexagon{sub II} phase transitions in the plasma membrane and subtending lamellae are manifestations of hydration-dependent bilayer-bilayer interactions.

Authors:
Publication Date:
Research Org.:
Cornell Univ., Ithaca, NY (United States). Dept. of Soil, Crop and Atmospheric Sciences
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
10166751
Report Number(s):
DOE/ER/13214-7
ON: DE92018399
DOE Contract Number:  
FG02-84ER13214
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: [1991]
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 09 BIOMASS FUELS; 60 APPLIED LIFE SCIENCES; FREEZING; BIOLOGICAL EFFECTS; CELL MEMBRANES; CONFORMATIONAL CHANGES; PROGRESS REPORT; RYE; LEAVES; PLANT CELLS; CRYOBIOLOGY; LAMELLAE; LIPIDS; OSMOSIS; SORBITOL; GLYCOLS; BIOLOGICAL ADAPTATION; 560400; 090800; 553000; OTHER ENVIRONMENTAL POLLUTANT EFFECTS; PRODUCTION; AGRICULTURE AND FOOD TECHNOLOGY

Citation Formats

Steponkus, P L. Effects of freezing and cold acclimation on the plasma membrane of isolated protoplasts. Summary progress report, May 16, 1987--June 1, 1991. United States: N. p., 1991. Web. doi:10.2172/10166751.
Steponkus, P L. Effects of freezing and cold acclimation on the plasma membrane of isolated protoplasts. Summary progress report, May 16, 1987--June 1, 1991. United States. https://doi.org/10.2172/10166751
Steponkus, P L. 1991. "Effects of freezing and cold acclimation on the plasma membrane of isolated protoplasts. Summary progress report, May 16, 1987--June 1, 1991". United States. https://doi.org/10.2172/10166751. https://www.osti.gov/servlets/purl/10166751.
@article{osti_10166751,
title = {Effects of freezing and cold acclimation on the plasma membrane of isolated protoplasts. Summary progress report, May 16, 1987--June 1, 1991},
author = {Steponkus, P L},
abstractNote = {This project focuses on lesions in the plasma membrane of protoplasts that occur during freezing to temperatures below {minus}5{degrees} which result in changes in the semipermeablity of the plasma membrane. This injury, referred to as loss of osmotic responsiveness, is associated with the formation of large, aparticulate domains in the plasma membrane, aparticulate lamellae subtending the plasma membrane, and lamellar-to-hexagonal{sub II} phase transitions in the plasma membrane and subtending lamellar. The goals of this project are to provide a mechanistic understanding of the mechanism by which freeze-induced dehydration effects the formation of aparticulate domains and lamellar-to-hexagonal{sub II} phase transitions and to determine the mechanisms by which cold acclimation and cryoprotectants preclude or diminish these ultrastructural changes. Our working hypothesis is the formation of aparticulate domains and lamellar-to-hexagon{sub II} phase transitions in the plasma membrane and subtending lamellae are manifestations of hydration-dependent bilayer-bilayer interactions.},
doi = {10.2172/10166751},
url = {https://www.osti.gov/biblio/10166751}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Dec 31 00:00:00 EST 1991},
month = {Tue Dec 31 00:00:00 EST 1991}
}