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Title: Designer proton-channel transgenic algae for photobiological hydrogen production

Patent ·
OSTI ID:1016564
 [1]
  1. Knoxville, TN
+ Show Author Affiliations

A designer proton-channel transgenic alga for photobiological hydrogen production that is specifically designed for production of molecular hydrogen (H.sub.2) through photosynthetic water splitting. The designer transgenic alga includes proton-conductive channels that are expressed to produce such uncoupler proteins in an amount sufficient to increase the algal H.sub.2 productivity. In one embodiment the designer proton-channel transgene is a nucleic acid construct (300) including a PCR forward primer (302), an externally inducible promoter (304), a transit targeting sequence (306), a designer proton-channel encoding sequence (308), a transcription and translation terminator (310), and a PCR reverse primer (312). In various embodiments, the designer proton-channel transgenic algae are used with a gas-separation system (500) and a gas-products-separation and utilization system (600) for photobiological H.sub.2 production.

Research Organization:
Lee, James Weifu (Knoxville, TN)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC05-00OR22725
Assignee:
Lee, James Weifu (Knoxville, TN)
Patent Number(s):
7,932,437
Application Number:
US Patent Application 11/748,531
OSTI ID:
1016564
Country of Publication:
United States
Language:
English

References (8)

Oxygen Tolerance of the H 2 -sensing [NiFe] Hydrogenase from Ralstonia eutropha H16 Is Based on Limited Access of Oxygen to the Active Site journal April 2005
Identification of Lhcb Gene Family Encoding the Light-harvesting Chlorophyll-a/b Proteins of Photosystem II in Chlamydomonas reinhardtii journal August 2001
Temperature effect on production of hydrogen and oxygen byChlamydomonas cold strain CCMP1619 and wild-type 137c: Scientific note journal September 1995
A New Oxygen Sensitivity and Its Potential Application in Photosynthetic H2 Production book January 2003
Coordinate Copper- and Oxygen-responsive Cyc6 and Cpx1 Expression in Chlamydomonas Is Mediated by the Same Element journal February 2000
Microalgae: a green source of renewable H2 journal December 2000
A New Perspective on Hydrogen Production by Photosynthetic Water Splitting book May 1997
Improvement of Photosynthetic CO2 Fixation at High Light Intensity Through Reduction of Chlorophyll Antenna Size journal January 2002

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60 APPLIED LIFE SCIENCES