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Title: Zymomonas with improved ethanol production in medium containing concentrated sugars and acetate

Patent ·
OSTI ID:1016555
 [1];  [2];  [3];  [4];  [5];  [6];  [2];  [7]
  1. Kennett Square, PA
  2. Lakewood, CO
  3. Centennial, CO
  4. Newark, DE
  5. Havertown, PA
  6. West Chester, PA
  7. New Hope, PA
+ Show Author Affiliations

Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas strains further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in mixed-sugars medium comprising xylose, and, in particular, in the presence of acetate.

Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC36-08GO28308
Assignee:
E.I. du Pont de Nemours and Company (Wilmington, DE); Alliance for Sustainable Energy LLC (
Patent Number(s):
7,897,396
Application Number:
US Patent Application 12/261,160
OSTI ID:
1016555
Country of Publication:
United States
Language:
English

References (15)

Growth inhibition ofZymomonas mobilis ATCC 10988 by ammonium ions journal June 1989
Performance of a newly developed integrant of Zymomonas mobilis for ethanol production on corn stover hydrolysate journal February 2004
Comparative Energetics of Glucose and Xylose Metabolism in Recombinant Zymomonas mobilis book January 2000
Integration host factor: A protein for all reasons journal November 1988
Global Gene Expression Profiling in Escherichia coli K12: THE EFFECTS OF INTEGRATION HOST FACTOR journal June 2000
The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4 journal December 2004
Global Analysis of Escherichia coli Gene Expression during the Acetate-Induced Acid Tolerance Response journal April 2001
Ethanol production from cellulose by coupled saccharification/fermentation usingSaccharomyces cerevisiae and cellulase complex fromSclerotiun rolfsii UV-8 mutant journal September 1992
Microbial Cellulose Utilization: Fundamentals and Biotechnology journal September 2002
Pentose metabolism in Zymomonas mobilis wild-type and recombinant strains journal December 1992
Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilis journal January 1995
Nuclear Magnetic Resonance Studies of Acetic Acid Inhibition of Rec Zymomonas mobilis ZM4(pZB5) journal January 2000
The effect of acetic acid on fuel ethanol production byZymomonas journal September 1993
Kinetic and Nuclear Magnetic Resonance Studies of Xylose Metabolism by Recombinant Zymomonas mobilisZM4(pZB5) journal January 2000
The role of integration host factor In gene expression in Escherichia coli journal September 1992

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60 APPLIED LIFE SCIENCES