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Title: Characterization of a defective interfering RNA that contains a mosaic of a plant viral genome. Final report

Abstract

Our lab was the first to describe and characterize a defective interfering RNA (DI RNAs or DIs) in association with a small RNA plant virus. The features of the DIs that we discovered in infections of tomato bushy stunt virus were compatible with the properties of DIs identified in many animal virus infections. Animal virologists have generally recognized the importance of studying DIs because they are invaluable tools for identifying cis-acting sequences important in virus multiplication and because they offer the opportunity to elucidate mechanisms involved in viral persistence and disease attenuation. Hence our discovery offered a comparably valuable tool for use in plant virus studies for the first time. Since then, we have also discovered the second example of plant viral DI RNAs associated with turnip crinkle virus (TCV), a virus structurally related to TBSV. We proposed a thorough characterization of this unique class of symptom modulating RNAs with the overall objective of identifying viral RNA nucleotide, sequences involved in such fundamental processes as virus replication and encapsidation as well as the degree of symptom expression resulting from the viral-DI-host interaction. The proposed research focused on the molecular characterization of the DI RNAs and the helper virus. We hadmore » demonstrated that the DIs were collinear deletion mutants of the genome of a cherry strain of tomato bushy stunt virus (TBSV). We had also shown that these low molecular weight RNAs interfered with the helper plant virus and modulated disease expression by preventing the development of a lethal necrotic disease in susceptible host plants. We also suggested that by exploring the mechanisms associated with the symptom attenuation effect, we might be able to devise novel strategies useful for engineering viral disease resistance.« less

Authors:
;
Publication Date:
Research Org.:
California Univ., Berkeley, CA (United States). Dept. of Plant Pathology
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
10151304
Report Number(s):
DOE/ER/13908-T1
ON: DE92015221
DOE Contract Number:  
FG03-88ER13908
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: [1991]
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PLANTS; DISEASE RESISTANCE; VIRUSES; DNA SEQUENCING; RNA; CLONING; PROGRESS REPORT; TRANSCRIPTION; 550200; 550400; BIOCHEMISTRY; GENETICS

Citation Formats

Morris, T J, and Jackson, A O. Characterization of a defective interfering RNA that contains a mosaic of a plant viral genome. Final report. United States: N. p., 1991. Web. doi:10.2172/10151304.
Morris, T J, & Jackson, A O. Characterization of a defective interfering RNA that contains a mosaic of a plant viral genome. Final report. United States. https://doi.org/10.2172/10151304
Morris, T J, and Jackson, A O. 1991. "Characterization of a defective interfering RNA that contains a mosaic of a plant viral genome. Final report". United States. https://doi.org/10.2172/10151304. https://www.osti.gov/servlets/purl/10151304.
@article{osti_10151304,
title = {Characterization of a defective interfering RNA that contains a mosaic of a plant viral genome. Final report},
author = {Morris, T J and Jackson, A O},
abstractNote = {Our lab was the first to describe and characterize a defective interfering RNA (DI RNAs or DIs) in association with a small RNA plant virus. The features of the DIs that we discovered in infections of tomato bushy stunt virus were compatible with the properties of DIs identified in many animal virus infections. Animal virologists have generally recognized the importance of studying DIs because they are invaluable tools for identifying cis-acting sequences important in virus multiplication and because they offer the opportunity to elucidate mechanisms involved in viral persistence and disease attenuation. Hence our discovery offered a comparably valuable tool for use in plant virus studies for the first time. Since then, we have also discovered the second example of plant viral DI RNAs associated with turnip crinkle virus (TCV), a virus structurally related to TBSV. We proposed a thorough characterization of this unique class of symptom modulating RNAs with the overall objective of identifying viral RNA nucleotide, sequences involved in such fundamental processes as virus replication and encapsidation as well as the degree of symptom expression resulting from the viral-DI-host interaction. The proposed research focused on the molecular characterization of the DI RNAs and the helper virus. We had demonstrated that the DIs were collinear deletion mutants of the genome of a cherry strain of tomato bushy stunt virus (TBSV). We had also shown that these low molecular weight RNAs interfered with the helper plant virus and modulated disease expression by preventing the development of a lethal necrotic disease in susceptible host plants. We also suggested that by exploring the mechanisms associated with the symptom attenuation effect, we might be able to devise novel strategies useful for engineering viral disease resistance.},
doi = {10.2172/10151304},
url = {https://www.osti.gov/biblio/10151304}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Dec 31 00:00:00 EST 1991},
month = {Tue Dec 31 00:00:00 EST 1991}
}