Characterization of a 1,4-{beta}-D-glucan synthase from Dictyostelium discoideum. Progress report, May 1990--January 1992
Various aspects of research concerning Dictyostelium discoideum are presented. The initial focus of this project was upon: the characterization of potential probes for the cellulose synthase (antibody and nucleic acid), the determination of the cultural induction conditions of cellulose synthesis, the solubilization of the enzyme activity, the development of a non-inhibitory disruption buffer, the generation and isolation of mutant strains deficient in cellulose synthesis, and the development of the capability to determine the degree of polymerization of the in vitro product. I have briefly summarized our most significant findings with only selected data sets being shown in this report in the interest of brevity.
- Research Organization:
- Texas Tech Univ., Lubbock, TX (United States). Dept. of Biological Sciences
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG05-90ER20006
- OSTI ID:
- 10138930
- Report Number(s):
- DOE/ER/20006-1; ON: DE92011937
- Resource Relation:
- Other Information: PBD: 15 Jan 1992
- Country of Publication:
- United States
- Language:
- English
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