Speciation of uranium after microbial action by XANES and XPS

Conference · Fri Dec 31 00:00:00 EST 1993

OSTI ID:10116420

Dodge, C J; Francis, A J; Lu, F ^[1]; Halada, G P; Kagwade, S V; Clayton, C R ^[2]

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1. Brookhaven National Lab., Upton, NY (United States)
2. State Univ. of New York, Stony Brook, NY (United States). Dept. of Materials Science

The speciation of radionuclides and toxic metals in wastes subjected to microbial action is important in determining the extent of stabilization in a disposal environment. As part of an ongoing study, we investigated the reduction of uranium by a Clostridium sp. using X-ray absorption neat edge spectroscopy (XANES) at the National Synchrotron Light Source (NSLS) and X-ray photoelectron spectroscopy (XPS). XPS analysis of uranyl acetate containing hexavalent uranium exhibited a binding energy of 382.0eV at the U 4f{sub 7/2} peak. The sample incubated in the presence of bacteria was shifted to lower binding energy (380.6eV), confirming the reduction of U{sup 6+} to U{sub 4+} at the bacterial surface. XANES analysis, using an electron yield detector, was performed at the M{sub v} absorption edge (3d-->5f). The absorption peak energy of the sample exhibited a shift from 3551.1eV to 3550.1eV which is higher than uranium metal (3549.6eV ) but lower than U{sup 4+} (3550.4eV). This indicates the presence of U{sup 3+} which is probably located beneath the surface within the biomass. Anaerobic bacterial treatment of wastes containing uranyl ion can result in the stabilization of uranium.

• Research Organization: Brookhaven National Lab., Upton, NY (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: AC02-76CH00016
• OSTI ID: 10116420
• Report Number(s): BNL-48856; CONF-930405-53; ON: DE94005450; TRN: 94:003495
• Resource Relation: Conference: Spring meeting of the Materials Research Society,San Francisco, CA (United States),12-16 Apr 1993; Other Information: PBD: [1993]
• Country of Publication: United States
• Language: English

Related Subjects

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE WASTES FROM NUCLEAR FACILITIES
59 BASIC BIOLOGICAL SCIENCES
URANIUM
BIODEGRADATION
SYNCHROTRON RADIATION
USES
CLOSTRIDIUM
RADIOACTIVE WASTE PROCESSING
X-RAY SPECTROSCOPY
PHOTOELECTRON SPECTROSCOPY
ABSORPTION SPECTROSCOPY
440103
052001
550500
NUCLEAR SPECTROSCOPIC INSTRUMENTATION
WASTE PROCESSING
METABOLISM