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  1. Chemiluminescence sulfur detection in capillary supercritical fluid chromatography

    The need for selective detectors in supercritical fluid chromatography (SFC) is particularly evident for the sensitive analysis of sulfur-containing species. These sulfur species are of interest to the environmental community, for the analysis of pesticides and atmospheric pollutants, as well as to the petroleum industry. Various detectors have been employed for the analysis of sulfur. The authors describe the use of a chemiluminescence sulfur detector (SCD) for the analysis of sulfur-containing organic analytes as separated by supercritical fluid chromatography. Detection limits of 35 and 115 pg of isopropylthiol and dodecanethiol, respectively, have been obtained for the SFC-SCD system with themore » linear dynamic range of about 10/sup 3/. Sulfur detectability appears to be independent of chromatographic pressure and the utility of the system is illustrated by the presentation of several real world analyses.« less
  2. A sensitive and rapid method for identification and characterization of low abundance receptors

    An improved method for detection of low intensity radioligand-receptor complexes resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is described. (3H)Azidopine-labeled 1,4-dihydropyridine (DHP) receptor from skeletal muscle resolved by SDS-PAGE was transferred to nitrocellulose and cut into strips and individual slices were analyzed for radioincorporation by liquid scintillation counting. (3H)Azidopine-labeled DHP binding subunit migrated as a single entity with a mass of 170 kDa and was confirmed using conventional methods. Results were obtained within 4 h after resolution by SDS-PAGE compared to 3-40 days using conventional methods. In addition, detection of extremely low signals (less than 50 cpm/lane), otherwise overwhelmedmore » by background noise using conventional methods, was possible due to removal of free ligand during electro-transfer to nitrocellulose. This technique offers a rapid sensitive, cost effective alternative to fluorography or other conventional gel slice analysis methods for detecting low intensity radiolabeled complexes resolved by SDS-PAGE.« less
  3. Dihydropyridine receptor of L-type Ca sup 2+ channels: Identification of binding domains for ( sup 3 H)(+)-PN200-110 and ( sup 3 H)azidopine within the. alpha. 1 subunit

    To identify the binding domain for dihydropyridine Ca{sup 2{plus}} antagonists, skeletal muscle Ca{sup 2{plus}} channels were photolabeled with ({sup 3}H)({plus})-PN200-110 and ({sup 3}H) azidopine. Regions of {alpha}1 photolabeled by these ligands were then identified by antibody mapping of proteolytic fragments. Approximately 50% of the specific labeling by both ligands was incorporated in domain III. ({sup 3}H)Azidopine labeled peptide Gln-989-Arg-1022, which contains a portion of the connecting loop between transmembrane segments IIIS5 and IIIS6 (IIIS5/S6), and peptide Ala-1023-Lys-1077, which contains IIIS6 itself and some adjacent amino acid residues. In contrast, ({sup 3}H)({plus})-PN200-110 labeling occurred almost exclusively in the fragment containing IIIS6.more » A second site labeled by both ligands was identified in transmembrane segment S6 of domain IV and adjacent residues. It is proposed, based on physiological studies, that these three peptide segments interact to form a receptor site accessible from the extracellular surface of the Ca{sup 2{plus}} channel.« less
  4. Energy conservation is a four-way street

    Nationwide, public utility commissions are struggling to develop novel approaches to promote energy conservation. Commissions are striving to guarantee that this decade is one of energy conservation and not lost opportunities. The commission, consumer advocate, utility, and consumer must in one way or another meet at the intersection of energy conservation. In the District of Columbia, there is a move to have these groups work cooperatively and collaboratively. These strategies have not always been successful; however, recently, given the additional experience and knowledge gained from least-cost planning, these groups have begun to understand the benefits of working together toward amore » common goal. If all join the cause, this decade will become the first of many decades of meaningful energy conservation.« less
  5. Transportation energy conservation data book: Edition 4

    This is the fourth edition of the Transportation Energy Conservation Data Book, a statistical compendium compiled and published by ORNL for DOE. Secondary data on transportation characteristics by mode, on transportation energy use, and on other related variables are presented in tabular and/or graphic form. All major modes of transportation are represented: highway, air, rail, marine, and pipeline. The six main chapters focus on various characteristics of the transportation sector including (1) modal characteristics, (2) current energy use, efficiency and conservation, (3) projections of modal energy use, (4) impact of government activities, (5) supply and cost of energy, and (6)more » general demographic and economic characteristics. Included in the tables and figures are the following transportation stock and use statistics: number of vehicles, vehicle-miles traveled, passenger-miles and freight ton-miles, fleet characteristics, household automobile ownership, size mix of automobiles, vehicle travel characteristics, and commuting patterns. Energy characteristics presented include energy use by fuel source and transportation mode, energy intensity figures by mode, indirect energy use, production as a percent of consumption, imports as a percent of domestic production, energy prices from the wellhead to the retail outlet, and alternative fuels.« less

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"Murphy, B.J."

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