Normalized cDNA libraries
Patent
·
OSTI ID:870993
- New York, NY
- Englewood, NJ
This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.
- Research Organization:
- Columbia Univ., New York, NY (United States)
- DOE Contract Number:
- FG02-91ER61233
- Assignee:
- Trustees of Columbia University in City of New York (New York, NY)
- Patent Number(s):
- US 5637685
- Application Number:
- 08/465811
- OSTI ID:
- 870993
- Country of Publication:
- United States
- Language:
- English
An ‘equalized cDNA library’ by the reassociation of short double-stranded cDNAs
|
journal | January 1990 |
Construction of a uniform-abundance (normalized) cDNA library.
|
journal | March 1991 |
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generating
fragments
complementary
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melting
reassociating
purified
moderate
cot
unassociated
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circle form
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