Radiation-induced gene responses
In the process of identifying genes that are differentially regulated in cells exposed to ultraviolet radiation (UV), we identified a transcript that was repressed following the exposure of cells to a combination of UV and salicylate, a known inhibitor of NF-kappaB. Sequencing this band determined that it has identify to lactate dehydrogenase, and Northern blots confirmed the initial expression pattern. Analysis of the sequence of the LDH 5` region established the presence of NF-kappaB, Sp1, and two Ap-2 elements; two partial AP- 1; one partial RE, and two halves of E-UV elements were also found. Electromobility shift assays were then performed for the AP-1, NF- kappaB, and E-UV elements. These experiments revealed that binding to NF-kappaB was induced by UV but repressed with salicylic acid; UV did not affect AP-1 binding, but salicylic acid inhibited it alone or following UV exposure; and E-UV binding was repressed by UV, and salicylic acid had little effect. Since the binding of no single element correlated with the expression pattern of LDH, it is likely that multiple elements govern UV/salicylate-mediated expression.
- Research Organization:
- Argonne National Lab., IL (United States). Center for Mechanistic Biology and Biotechnology
- Sponsoring Organization:
- USDOE Office of Energy Research, Washington, DC (United States); National Insts. of Health, Bethesda, MD (United States)
- DOE Contract Number:
- W-31109-ENG-38
- OSTI ID:
- 477624
- Report Number(s):
- ANL/CMB/CP-91543; CONF-961150-1; ON: DE97001397; CNN: Grant CA73042; TRN: 97:010891
- Resource Relation:
- Conference: International conference on radiation and health, Beer Sheva (Israel), 3-7 Nov 1996; Other Information: PBD: [1996]
- Country of Publication:
- United States
- Language:
- English
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