High-yield hydrogen production from biomass by in vitro metabolic engineering: Mixed sugars coutilization and kinetic modeling

Rollin, Joseph A.; Martin del Campo, Julia; Myung, Suwan; Sun, Fangfang; You, Chun; Bakovic, Allison; Castro, Roberto; Chandrayan, Sanjeev K.; Wu, Chang-Hao; Adams, Michael W.  W.; Senger, Ryan S.; Zhang, Y. -H.  Percival

doi:10.1073/pnas.1417719112

Title: High-yield hydrogen production from biomass by in vitro metabolic engineering: Mixed sugars coutilization and kinetic modeling

Journal Article · Mon Apr 06 00:00:00 EDT 2015 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1417719112· OSTI ID:1293980

Rollin, Joseph A. ^[1]; Martin del Campo, Julia ^[2]; Myung, Suwan ^[3]; Sun, Fangfang ^[4]; You, Chun ^[1]; Bakovic, Allison ^[5]; Castro, Roberto ^[6]; Chandrayan, Sanjeev K. ^[7]; Wu, Chang-Hao ^[7]; Adams, Michael W. W. ^[8]; Senger, Ryan S. ^[2]; Zhang, Y. -H. Percival ^[9]

Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Dept. of Biological Systems Engineering; Cell-Free Bioinnovations, Blacksburg, VA (United States)
Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Dept. of Biological Systems Engineering
Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Dept. of Biological Systems Engineering; Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Inst. for Critical Technology and Applied Science
Cell-Free Bioinnovations, Blacksburg, VA (United States)
Milwaukee School of Engineering, Milwaukee, WI (United States). Dept. of Physics and Chemistry
Texas A&M Univ., Kingsville, TX (United States). Dept. of Chemical and Natural Gas Engineering
Univ. of Georgia, Athens, GA (United States). Dept. of Biochemistry and Molecular Biology
Univ. of Georgia, Athens, GA (United States). Dept. of Biochemistry and Molecular Biology; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Dept. of Biological Systems Engineering; Cell-Free Bioinnovations, Blacksburg, VA (United States); Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Inst. for Critical Technology and Applied Science; Chinese Academy of Sciences (CAS), Tianjin(China). Tianjin Inst. of Industrial Biotechnology

The use of hydrogen (H₂) as a fuel offers enhanced energy conversion efficiency and tremendous potential to decrease greenhouse gas emissions, but producing it in a distributed, carbon-neutral, low-cost manner requires new technologies. Herein we demonstrate the complete conversion of glucose and xylose from plant biomass to H₂ and CO₂ based on an in vitro synthetic enzymatic pathway. Glucose and xylose were simultaneously converted to H₂ with a yield of two H₂ per carbon, the maximum possible yield. Parameters of a nonlinear kinetic model were fitted with experimental data using a genetic algorithm, and a global sensitivity analysis was used to identify the enzymes that have the greatest impact on reaction rate and yield. After optimizing enzyme loadings using this model, volumetric H₂ productivity was increased 3-fold to 32 mmol H₂∙L^₋1∙h^₋1. The productivity was further enhanced to 54 mmol H₂∙L^₋1∙h^₋1 by increasing reaction temperature, substrate, and enzyme concentrations—an increase of 67-fold compared with the initial studies using this method. The production of hydrogen from locally produced biomass is a promising means to achieve global green energy production.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: FG05-95ER20175; IIP-1321528; IIP-1353266

OSTI ID:: 1293980

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 16; ISSN 0027-8424

Publisher:: National Academy of Sciences, Washington, DC (United States)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 159 works

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Title: High-yield hydrogen production from biomass by in vitro metabolic engineering: Mixed sugars coutilization and kinetic modeling

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