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Title: Forming giant-sized polymersomes using gel-assisted rehydration

Journal Article · · Journal of Visualized Experiments
DOI:https://doi.org/10.3791/54051· OSTI ID:1257838
 [1];  [2];  [1]
  1. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
  2. Sandia National Lab. (SNL-CA), Livermore, CA (United States)

Here, we present a protocol to rapidly form giant polymer vesicles (pGVs). Briefly, polymer solutions are dehydrated on dried agarose films adhered to coverslips. Rehydration of the polymer films results in rapid formation of pGVs. This method greatly advances the preparation of synthetic giant vesicles for direct applications in biomimetic studies.

Research Organization:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Sponsoring Organization:
USDOE
Grant/Contract Number:
AC04-94AL85000
OSTI ID:
1257838
Report Number(s):
SAND-2016-2307J; jove; 621961
Journal Information:
Journal of Visualized Experiments, Journal Issue: 111; ISSN 1940-087X
Publisher:
MyJoVE Corp.Copyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 6 works
Citation information provided by
Web of Science

References (4)

Integrin reconstituted in GUVs: A biomimetic system to study initial steps of cell spreading journal October 2009
Biocompatible Functionalization of Polymersome Surfaces: A New Approach to Surface Immobilization and Cell Targeting Using Polymersomes journal March 2011
Films of Agarose Enable Rapid Formation of Giant Liposomes in Solutions of Physiologic Ionic Strength journal February 2009
Polymersomes: Tough Vesicles Made from Diblock Copolymers journal May 1999