Bimodal effect of hydrogen peroxide and oxidative events in nitrite-induced rapid root abscission by the water fern Azolla pinnata

Cohen, Michael F.; Gurung, Sushma; Birarda, Giovanni; Holman, Hoi-Ying N.; Yamasaki, Hideo

doi:10.3389/fpls.2015.00518

Title: Bimodal effect of hydrogen peroxide and oxidative events in nitrite-induced rapid root abscission by the water fern Azolla pinnata

Journal Article · Thu Jul 09 00:00:00 EDT 2015 · Frontiers in Plant Science

DOI:https://doi.org/10.3389/fpls.2015.00518· OSTI ID:1257258

Cohen, Michael F. ^[1]; Gurung, Sushma ^[2]; Birarda, Giovanni ^[3]; Holman, Hoi-Ying N. ^[3]; Yamasaki, Hideo ^[2]

Sonoma State Univ., Rohnert Park, CA (United States); Okinawa Inst. of Science and Technology, Okinawa (Japan)
Univ. of the Ryukyus, Nishihara (Japan)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

In the genus Azolla rapid abscission of roots from floating fronds occurs within minutes in response to a variety of stresses, including exposure to nitrite. We found that hydrogen peroxide, though itself not an inducer of root abscission, modulates nitrite-induced root abscission by Azolla pinnata in a dose-dependent manner, with 2 mM H₂O₂ significantly diminishing the responsiveness to 2 mM NaNO₂, and 10 mM H₂O₂ slightly enhancing it. Hypoxia, which has been found in other plants to result in autogenic production of H₂O₂, dramatically stimulated root abscission of A. pinnata in response to nitrite, especially for plants previously cultivated in medium containing 5 mM KNO₃ compared to plants cultivated under N₂-fixing conditions without combined nitrogen. Plants, including Azolla, produce the small signaling molecule nitric oxide (NO) from nitrite using nitrate reductase. We found Azolla plants to display dose-dependent root abscission in response to the NO donor spermine NONOate. Treatment of plants with the thiol-modifying agents S-methyl methanethiosulfonate or glutathione inhibited the nitrite-induced root abscission response. Synchrotron radiation-based Fourier transform infrared spectromicroscopy revealed higher levels of carbonylation in the abscission zone of dropped roots, indicative of reaction products of polysaccharides with potent free radical oxidants. Lastly, we hypothesize that metabolic products of nitrite and NO react with H₂O₂ in the apoplast leading to free-radical-mediated cleavage of structural polysaccharides and consequent rapid root abscission.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1257258

Journal Information:: Frontiers in Plant Science, Vol. 6; ISSN 1664-462X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 3 works

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d-Cysteine-Induced Rapid Root Abscission in the Water Fern Azolla Pinnata: Implications for the Linkage between d-Amino Acid and Reactive Sulfur Species (RSS) in Plant Environmental Responses Yamasaki, Hideo; Ogura, Masahiro P.; Kingjoe, Katsumi A. Antioxidants, Vol. 8, Issue 9 https://doi.org/10.3390/antiox8090411	journal	September 2019

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59 BASIC BIOLOGICAL SCIENCES
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
root abscission
apoplast
free radical cleavage
FTIR spectromicroscopy
hydrogen peroxide
nitric oxide
nitrite
plant cell wall loosening

Title: Bimodal effect of hydrogen peroxide and oxidative events in nitrite-induced rapid root abscission by the water fern Azolla pinnata

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