skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: High quality draft genome sequence of Leucobacter chironomi strain MM2LBT (DSM 19883T) isolated from a Chironomus sp. egg mass

Journal Article · · Standards in Genomic Sciences
 [1];  [2];  [3];  [3];  [3];  [3];  [3];  [4];  [5];  [5];  [3];  [6];  [1]
  1. Univ. of Haifa (Israel)
  2. St. Petersburg Academic Univ. (Russia); St. Petersburg Academic Univ. (Russia)
  3. Dept. of Energy Joint Genome Inst., Walnut Creek, CA (United States)
  4. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  5. Leibniz Institute DSMZ, Braunschweig (Germany)
  6. Dept. of Energy Joint Genome Inst., Walnut Creek, CA (United States); King Abdulaziz Univ., Jeddah (Saudi Arabia)
+ Show Author Affiliations

Leucobacter chironomi strain MM2LBT (Halpern et al., Int J Syst Evol Microbiol 59:665-70 2009) is a Gram-positive, rod shaped, non-motile, aerobic, chemoorganotroph bacterium. L. chironomi belongs to the family Microbacteriaceae, a family within the class Actinobacteria. Strain MM2LBT was isolated from a chironomid (Diptera; Chironomidae) egg mass that was sampled from a waste stabilization pond in northern Israel. In a phylogenetic tree based on 16S rRNA gene sequences, strain MM2LBT formed a distinct branch within the radiation encompassing the genus Leucobacter. Here we describe the features of this organism, together with the complete genome sequence and annotation. We find that the DNA GC content is 69.90%. The chromosome length is 2,964,712 bp. It encodes 2,690 proteins and 61 RNA genes. L. chironomi genome is part of the Genomic Encyclopedia of Type Strains, Phase I: the one thousand microbial genomes (KMG) project.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1256905
Journal Information:
Standards in Genomic Sciences, Vol. 10, Issue 1; ISSN 1944-3277
Publisher:
BioMed CentralCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 6 works
Citation information provided by
Web of Science

References (50)

Proposal for a New Hierarchic Classification System, Actinobacteria classis nov. journal April 1997
The DOE-JGI Standard Operating Procedure for the Annotations of Microbial Genomes journal July 2009
Re-identification of Aeromonas isolates from chironomid egg masses as the potential pathogenic bacteria Aeromonas aquariorum: Aeromonas aquarirum in chironomid eggs journal October 2010
Identification of novel biomass-degrading enzymes from genomic dark matter: Populating genomic sequence space with functional annotation: Context-Guided Identification of Novel Cellulases journal May 2014
Leucobacter tardus sp. nov., isolated from the phyllosphere of Solanum tuberosum L. journal November 2008
The DNA Bank Network: The Start from a German Initiative journal March 2011
Draft Genome Sequence of Leucobacter chromiiresistens, an Extremely Chromium-Tolerant Strain journal December 2011
Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB: List No. 53 journal April 1995
Chironomid Egg Masses as a Natural Reservoir of Vibrio cholerae Non-O1 and Non-O139 in Freshwater Habitats journal December 2003
Leucobacter chironomi sp. nov., a chromate-resistant bacterium isolated from a chironomid egg mass journal April 2009
High-quality draft assemblies of mammalian genomes from massively parallel sequence data journal December 2010
Leucobacter iarius sp. nov., in the family Microbacteriaceae journal April 2007
Approved Lists of Bacterial Names journal January 1980
The protective role of endogenous bacterial communities in chironomid egg masses and larvae journal June 2013
Solexa Ltd journal June 2004
Chironomid egg masses and Vibrio cholerae journal July 2001
Vibrio cholerae and Aeromonas: do they share a mutual host? journal February 2008
The Genomic Standards Consortium journal June 2011
Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya. journal June 1990
Genomic Encyclopedia of Type Strains, Phase I: The one thousand microbial genomes (KMG-I) project journal December 2013
Prodigal: prokaryotic gene recognition and translation initiation site identification journal March 2010
Leucobacter celer sp. nov., isolated from Korean fermented seafood journal October 2010
Genome sequence of the chromate-resistant bacterium Leucobacter salsicius type strain M1-8T journal December 2013
Leucobacter komagatae gen. nov., sp. nov., a New Aerobic Gram-Positive, Nonsporulating Rod with 2,4-Diaminobutyric Acid in the Cell Wall journal October 1996
Leucobacter chromiireducens subsp. solipictus subsp. nov., a pigmented bacterium isolated from the nematode Caenorhabditis elegans, and emended description of L. chromiireducens journal December 2007
Chironomid egg masses harbour the clinical species Aeromonas taiwanensis and Aeromonas sanarellii journal October 2012
Velvet: Algorithms for de novo short read assembly using de Bruijn graphs journal February 2008
Culturable and VBNC Vibrio cholerae: Interactions with Chironomid Egg Masses and Their Bacterial Population journal December 2006
An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa journal March 2009
Leucobacter aerolatus sp. nov., from the air of a duck barn journal January 2010
The Genomes OnLine Database (GOLD) v.5: a metadata management system based on a four level (meta)genome project classification journal October 2014
Bacterial Community Composition Associated with Chironomid Egg Masses journal December 2012
Leucobacter exalbidus sp. nov., an actinobacterium isolated from a mixed culture from compost journal January 2011
Phylogeny-driven target selection for large-scale genome-sequencing (and other) projects journal May 2013
Leucobacter luti sp. nov., and Leucobacter alluvii sp. nov., two new species of the genus Leucobacter isolated under chromium stress journal July 2006
MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods journal May 2011
The minimum information about a genome sequence (MIGS) specification journal May 2008
Leucobacter denitrificans sp. nov., isolated from cow dung journal February 2012
Dependent population dynamics between chironomids (nonbiting midges) and Vibrio cholerae: Chironomids and Vibrio cholerae journal January 2006
Suprageneric classification of peptidoglycan group B actinomycetes by nucleotide sequencing of 5S ribosomal RNA journal January 1994
The Fast Changing Landscape of Sequencing Technologies and Their Impact on Microbial Genome Assemblies and Annotation journal December 2012
IMG ER: a system for microbial genome annotation expert review and curation journal June 2009
En route to a genome-based classification of Archaea and Bacteria? journal June 2010
A phylogeny-driven genomic encyclopaedia of Bacteria and Archaea journal December 2009
Genomic Encyclopedia of Bacteria and Archaea: Sequencing a Myriad of Type Strains journal August 2014
Improving Microbial Genome Annotations in an Integrated Database Context journal February 2013
Studies in the Nomenclature and Classification of the Bacteria ii. the Primary Subdivisions of the Schizomycetes journal January 1917
Draft Genome Sequence of Leucobacter sp. Strain UCD-THU (Phylum Actinobacteria ) journal June 2013
Genome-wide identification, structural analysis and expression profiles of GRAS gene family in orchardgrass journal February 2020
PICRUSt2 for prediction of metagenome functions journal June 2020

Cited By (4)

Exemplar Abstract for Leucobacter chironomi Halpern et al. 2009 emend. Nouioui et al. 2018. dataset January 2013
Antibiotic resistance genes in the Actinobacteria phylum journal June 2019
Comparative genomics reveals a novel genetic organization of the sad cluster in the sulfonamide-degrader ‘Candidatus Leucobacter sulfamidivorax’ strain GP journal November 2019
High-Quality Draft Genome Sequence of Leucobacter sp. Strain G161, a Distinct and Effective Chromium Reducer journal February 2016

Similar Records

High quality draft genome sequence of Brachymonas chironomi AIMA4T (DSM 19884T) isolated from a Chironomus sp. egg mass
Journal Article · Wed May 27 00:00:00 EDT 2015 · Standards in Genomic Sciences · OSTI ID:1256905
+13 more
High quality permanent draft genome sequence of Phaseolibacter flectens ATCC 12775T, a plant pathogen of French bean pods
Journal Article · Wed Jan 13 00:00:00 EST 2016 · Standards in Genomic Sciences · OSTI ID:1256905
+10 more
High quality permanent draft genome sequence of Chryseobacterium bovis DSM 19482T, isolated from raw cow milk
Journal Article · Mon May 08 00:00:00 EDT 2017 · Standards in Genomic Sciences · OSTI ID:1256905
+15 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Leucobacter chironomi
Microbacteriaceae
Chironomid
Chironomus
Egg mass
Hexavalent chromium