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Title: The Stanford Automated Mounter: Pushing the limits of sample exchange at the SSRL macromolecular crystallography beamlines

Abstract

The Stanford Automated Mounter System, a system for mounting and dismounting cryo-cooled crystals, has been upgraded to increase the throughput of samples on the macromolecular crystallography beamlines at the Stanford Synchrotron Radiation Lightsource. This upgrade speeds up robot maneuvers, reduces the heating/drying cycles, pre-fetches samples and adds an air-knife to remove frost from the gripper arms. As a result, sample pin exchange during automated crystal quality screening now takes about 25 s, five times faster than before this upgrade.

Authors:
 [1];  [1];  [1];  [1]
  1. SLAC National Accelerator Lab., Menlo Park, CA (United States)
Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1253923
Report Number(s):
SLAC-PUB-16477
Journal ID: ISSN 1600-5767; JACGAR; PII: S1600576716000649
Grant/Contract Number:  
AC02-76SF00515
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Journal of Applied Crystallography (Online)
Additional Journal Information:
Journal Volume: 49; Journal Issue: 2; Journal ID: ISSN 1600-5767
Publisher:
International Union of Crystallography
Country of Publication:
United States
Language:
English
Subject:
43 PARTICLE ACCELERATORS; automated crystal mounting; high-throughput sample exchange; sample exchange robot; synchrotron robotics; protein crystallography; remote access; cryo-crystallography

Citation Formats

Russi, Silvia, Song, Jinhu, McPhillips, Scott E., and Cohen, Aina E. The Stanford Automated Mounter: Pushing the limits of sample exchange at the SSRL macromolecular crystallography beamlines. United States: N. p., 2016. Web. doi:10.1107/S1600576716000649.
Russi, Silvia, Song, Jinhu, McPhillips, Scott E., & Cohen, Aina E. The Stanford Automated Mounter: Pushing the limits of sample exchange at the SSRL macromolecular crystallography beamlines. United States. https://doi.org/10.1107/S1600576716000649
Russi, Silvia, Song, Jinhu, McPhillips, Scott E., and Cohen, Aina E. 2016. "The Stanford Automated Mounter: Pushing the limits of sample exchange at the SSRL macromolecular crystallography beamlines". United States. https://doi.org/10.1107/S1600576716000649. https://www.osti.gov/servlets/purl/1253923.
@article{osti_1253923,
title = {The Stanford Automated Mounter: Pushing the limits of sample exchange at the SSRL macromolecular crystallography beamlines},
author = {Russi, Silvia and Song, Jinhu and McPhillips, Scott E. and Cohen, Aina E.},
abstractNote = {The Stanford Automated Mounter System, a system for mounting and dismounting cryo-cooled crystals, has been upgraded to increase the throughput of samples on the macromolecular crystallography beamlines at the Stanford Synchrotron Radiation Lightsource. This upgrade speeds up robot maneuvers, reduces the heating/drying cycles, pre-fetches samples and adds an air-knife to remove frost from the gripper arms. As a result, sample pin exchange during automated crystal quality screening now takes about 25 s, five times faster than before this upgrade.},
doi = {10.1107/S1600576716000649},
url = {https://www.osti.gov/biblio/1253923}, journal = {Journal of Applied Crystallography (Online)},
issn = {1600-5767},
number = 2,
volume = 49,
place = {United States},
year = {Wed Feb 24 00:00:00 EST 2016},
month = {Wed Feb 24 00:00:00 EST 2016}
}

Journal Article:
Free Publicly Available Full Text
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Cited by: 22 works
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Works referenced in this record:

High-density grids for efficient data collection from multiple crystals
journal, January 2016


Beamline automation and remote access at NSRRC BL-13
journal, August 2008


An automated system to mount cryo-cooled protein crystals on a synchrotron beamline, using compact sample cassettes and a small-scale robot
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Goniometer-based femtosecond crystallography with X-ray free electron lasers
journal, October 2014


MX1: a bending-magnet crystallography beamline serving both chemical and macromolecular crystallography communities at the Australian Synchrotron
journal, January 2015


Canadian macromolecular crystallography facility: a suite of fully automated beamlines
journal, January 2012


High-throughput operation of sample-exchange robots with double tongs at the Photon Factory beamlines
journal, April 2008


Improvement of an automated protein crystal exchange system PAM for high-throughput data collection
journal, October 2013


Capture and X-ray diffraction studies of protein microcrystals in a microfluidic trap array
journal, March 2015


Automated technologies and novel techniques to accelerate protein crystallography for structural genomics
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