Method to produce acetyldiacylglycerols (ac-TAGs) by expression of an acetyltransferase gene isolated from Euonymus alatus (burning bush)

Durrett, Timothy; Ohlrogge, John; Pollard, Michael

Title: Method to produce acetyldiacylglycerols (ac-TAGs) by expression of an acetyltransferase gene isolated from Euonymus alatus (burning bush)

Patent · Tue May 03 00:00:00 EDT 2016

OSTI ID:1250460

Durrett, Timothy; Ohlrogge, John; Pollard, Michael

The present invention relates to novel diacylglycerol acyltransferase genes and proteins, and methods of their use. In particular, the invention describes genes encoding proteins having diacylglycerol acetyltransferase activity, specifically for transferring an acetyl group to a diacylglycerol substrate to form acetyl-Triacylglycerols (ac-TAGS), for example, a 3-acetyl-1,2-diacyl-sn-glycerol. The present invention encompasses both native and recombinant wild-type forms of the transferase, as well as mutants and variant forms. The present invention also relates to methods of using novel diacylglycerol acyltransferase genes and proteins, including their expression in transgenic organisms at commercially viable levels, for increasing production of 3-acetyl-1,2-diacyl-sn-glycerols in plant oils and altering the composition of oils produced by microorganisms, such as yeast, by increasing ac-TAG production. Additionally, oils produced by methods of the present inventions comprising genes and proteins are contemplated for use as biodiesel fuel, in polymer production and as naturally produced food oils with reduced calories.

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Research Organization:: Michigan State Univ., East Lansing, MI (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FC02-07ER64494

Assignee:: Board of Trustees of Michigan State University (East Lansing, MI)

Patent Number(s):: 9,328,335

Application Number:: 13/519,660

OSTI ID:: 1250460

Resource Relation:: Patent File Date: 2010 Dec 29

Country of Publication:: United States

Language:: English

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Enzyme directed oil biosynthesis in microalgae Benning, Christoph; Miller, Rachel; Moellering, Eric R. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8362318 US Patent Document 8,362,318	patent	January 2013
Enzyme directed oil biosynthesis in microalgae Benning, Christoph; Miller, Rachel; Moellering, Eric R. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9062331 US Patent Document 9,062,331	patent	June 2015
Diacylglycerol Acyltransferase Genes, Proteins, and Uses Thereof Milcamps, Anne; Pan, David A.; Pollard, Michael R. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070028329 US Patent Application 11/541881; 20070028329	patent-application	February 2007
Fatty Acid Elongase (FAE) Genes and Their Utility in Increasing Erucic Acid and Other Very Long-Chain Fatty Acid Proportions in Seed Oil Mietkiewska, Elzbieta; Taylor, David C.; Katavic, Vesna https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070204370 US Patent Application 10/596024; 20070204370	patent-application	August 2007
Targeted and Non-Targeted Gene Insertions Using a Linear Material Element Construct Lawrence, Christopher; Cho, Yangrae https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070231819 US Patent Application 11/686589; 20070231819	patent-application	October 2007
Molecular Approaches for the Optimization of Biofuel Production Sayre, Richard T.; Pereira, Suzette L. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20100317073 US Patent Application 12/743434; 20100317073	patent-application	December 2010
Diacylglycerol Acyltransferase 2 Genes and Proteins Encoded Thereby from Algae Zou, Jitao; Xu, Jingyu; Zheng, Zhifu https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20110061130 US Patent Application 12/735132; 20110061130	patent-application	March 2011
Enzyme Directed Oil Biosynthesis in Microalgae Benning, Christoph; Miller, Rachel; Moellering, Eric R. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20130212736 US Patent Application 13/719868; 20130212736	patent-application	August 2013

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