Thermostable cellulases, and mutants thereof, capable of hydrolyzing cellulose in ionic liquid

Sapra, Rajat; Datta, Supratim; Chen, Zhiwei; Holmes, Bradley M.; Simmons, Blake A.; Blanch, Harvey W.

Title: Thermostable cellulases, and mutants thereof, capable of hydrolyzing cellulose in ionic liquid

Patent · Tue Apr 26 00:00:00 EDT 2016

OSTI ID:1248892

Sapra, Rajat; Datta, Supratim; Chen, Zhiwei; Holmes, Bradley M.; Simmons, Blake A.; Blanch, Harvey W.

The present invention provides for a composition comprising an ionic liquid and a thermostable cellulose, and a method of hydrolyzing a cellulose, comprising: (a) providing a composition comprising a solution comprising an ionic liquid and a cellulose, and (b) introducing a thermostable cellulase to the solution, such that the cellulose is hydrolyzed by the cellulase. The present invention also provides for a Thermatoga maritima thermostable cellulase mutant with increased cellulase activity.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC02-05CH11231

Assignee:: The Regents of the University of California (Oakland, CA) Sandia Corporation (Livermore, CA)

Patent Number(s):: 9,322,042

Application Number:: 13/265,786

OSTI ID:: 1248892

Resource Relation:: Patent File Date: 2010 Apr 23

Country of Publication:: United States

Language:: English

References (9)

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Carboxymethyl cellulase fromthermotoga maritima Mathur, Eric J.; Lam, David E. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5962258 US Patent Document 5,962,258	patent	October 1999
Carboxymethyl cellulase from Thermotoga maritima Mathur, Eric J.; Lam, David E. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6008032 US Patent Document 6,008,032	patent	December 1999
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Carboxymethyl cellulose from thermotoga maritima Mathur, Eric J.; Lam, David E. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6245547 US Patent Document 6,245,547	patent	June 2001
Methods for generating cellulases Short, Jay M.; Mathur, Eric J.; Lam, David E. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7422876 US Patent Document 7,422,876	patent	September 2008
Thermostable cellulase and methods of use Brumm, Phillip J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7510857 US Patent Document 7,510,857	patent	March 2009
Biomass pretreatment Varanasi, Sasidhar; Schall, Constance Ann; Dadi, Anantharam Prasad https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20080227162 US Patent Application 12/075762; 20080227162	patent-application	September 2008

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