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Title: Quantitative metrics for assessment of chemical image quality and spatial resolution

Abstract

Rationale: Currently objective/quantitative descriptions of the quality and spatial resolution of mass spectrometry derived chemical images are not standardized. Development of these standardized metrics is required to objectively describe chemical imaging capabilities of existing and/or new mass spectrometry imaging technologies. Such metrics would allow unbiased judgment of intra-laboratory advancement and/or inter-laboratory comparison for these technologies if used together with standardized surfaces. Methods: We developed two image metrics, viz., chemical image contrast (ChemIC) based on signal-to-noise related statistical measures on chemical image pixels and corrected resolving power factor (cRPF) constructed from statistical analysis of mass-to-charge chronograms across features of interest in an image. These metrics, quantifying chemical image quality and spatial resolution, respectively, were used to evaluate chemical images of a model photoresist patterned surface collected using a laser ablation/liquid vortex capture mass spectrometry imaging system under different instrument operational parameters. Results: The calculated ChemIC and cRPF metrics determined in an unbiased fashion the relative ranking of chemical image quality obtained with the laser ablation/liquid vortex capture mass spectrometry imaging system. These rankings were used to show that both chemical image contrast and spatial resolution deteriorated with increasing surface scan speed, increased lane spacing and decreasing size of surface features. Conclusions:more » ChemIC and cRPF, respectively, were developed and successfully applied for the objective description of chemical image quality and spatial resolution of chemical images collected from model surfaces using a laser ablation/liquid vortex capture mass spectrometry imaging system.« less

Authors:
 [1];  [1];  [1]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Chemical Sciences Division
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1240566
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Rapid Communications in Mass Spectrometry
Additional Journal Information:
Journal Volume: 30; Journal Issue: 7; Journal ID: ISSN 0951-4198
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Kertesz, Vilmos, Cahill, John F., and Van Berkel, Gary J. Quantitative metrics for assessment of chemical image quality and spatial resolution. United States: N. p., 2016. Web. doi:10.1002/rcm.7519.
Kertesz, Vilmos, Cahill, John F., & Van Berkel, Gary J. Quantitative metrics for assessment of chemical image quality and spatial resolution. United States. https://doi.org/10.1002/rcm.7519
Kertesz, Vilmos, Cahill, John F., and Van Berkel, Gary J. 2016. "Quantitative metrics for assessment of chemical image quality and spatial resolution". United States. https://doi.org/10.1002/rcm.7519. https://www.osti.gov/servlets/purl/1240566.
@article{osti_1240566,
title = {Quantitative metrics for assessment of chemical image quality and spatial resolution},
author = {Kertesz, Vilmos and Cahill, John F. and Van Berkel, Gary J.},
abstractNote = {Rationale: Currently objective/quantitative descriptions of the quality and spatial resolution of mass spectrometry derived chemical images are not standardized. Development of these standardized metrics is required to objectively describe chemical imaging capabilities of existing and/or new mass spectrometry imaging technologies. Such metrics would allow unbiased judgment of intra-laboratory advancement and/or inter-laboratory comparison for these technologies if used together with standardized surfaces. Methods: We developed two image metrics, viz., chemical image contrast (ChemIC) based on signal-to-noise related statistical measures on chemical image pixels and corrected resolving power factor (cRPF) constructed from statistical analysis of mass-to-charge chronograms across features of interest in an image. These metrics, quantifying chemical image quality and spatial resolution, respectively, were used to evaluate chemical images of a model photoresist patterned surface collected using a laser ablation/liquid vortex capture mass spectrometry imaging system under different instrument operational parameters. Results: The calculated ChemIC and cRPF metrics determined in an unbiased fashion the relative ranking of chemical image quality obtained with the laser ablation/liquid vortex capture mass spectrometry imaging system. These rankings were used to show that both chemical image contrast and spatial resolution deteriorated with increasing surface scan speed, increased lane spacing and decreasing size of surface features. Conclusions: ChemIC and cRPF, respectively, were developed and successfully applied for the objective description of chemical image quality and spatial resolution of chemical images collected from model surfaces using a laser ablation/liquid vortex capture mass spectrometry imaging system.},
doi = {10.1002/rcm.7519},
url = {https://www.osti.gov/biblio/1240566}, journal = {Rapid Communications in Mass Spectrometry},
issn = {0951-4198},
number = 7,
volume = 30,
place = {United States},
year = {Sun Feb 28 00:00:00 EST 2016},
month = {Sun Feb 28 00:00:00 EST 2016}
}

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  • Ovchinnikova, Olga S.; Bhandari, Deepak; Lorenz, Matthias
  • Rapid Communications in Mass Spectrometry, Vol. 28, Issue 15, p. 1665-1673
  • https://doi.org/10.1002/rcm.6946

Works referencing / citing this record:

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