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Title: Determination of terpenoid content in pine by organic solvent extraction and fast-GC analysis

Journal Article · · Frontiers in Energy Research
 [1];  [1];  [2];  [1]
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States)
  2. Univ. of Florida, Gainesville, FL (United States)
+ Show Author Affiliations

Terpenoids, naturally occurring compounds derived from isoprene units present in pine oleoresin, are a valuable source of chemicals used in solvents, fragrances, flavors, and have shown potential use as a biofuel. This paper describes a method to extract and analyze the terpenoids present in loblolly pine saplings and pine lighter wood. Various extraction solvents were tested over different times and temperatures. Samples were analyzed by pyrolysis-molecular beam mass spectrometry before and after extractions to monitor the extraction efficiency. The pyrolysis studies indicated that the optimal extraction method used a 1:1 hexane/acetone solvent system at 22°C for 1 h. Extracts from the hexane/acetone experiments were analyzed using a low thermal mass modular accelerated column heater for fast-GC/FID analysis. The most abundant terpenoids from the pine samples were quantified, using standard curves, and included the monoterpenes, α- and β-pinene, camphene, and δ-carene. Sesquiterpenes analyzed included caryophyllene, humulene, and α-bisabolene. In conclusion, diterpenoid resin acids were quantified in derivatized extractions, including pimaric, isopimaric, levopimaric, palustric, dehydroabietic, abietic, and neoabietic acids.

Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE Advanced Research Projects Agency - Energy (ARPA-E)
Grant/Contract Number:
AC36-08GO28308
OSTI ID:
1239644
Report Number(s):
NREL/JA-5100-64186
Journal Information:
Frontiers in Energy Research, Vol. 4, Issue 1; ISSN 2296-598X
Publisher:
Frontiers Research FoundationCopyright Statement
Country of Publication:
United States
Language:
English

References (24)

Sesquiterpenoid Emissions from Agricultural Crops: Correlations to Monoterpenoid Emissions and Leaf Terpene Content journal May 2010
Terpenoid biomaterials journal May 2008
Efficient conversion of pure and mixed terpene feedstocks to high density fuels journal July 2012
Pine oil–biodiesel blends: A double biofuel strategy to completely eliminate the use of diesel in a diesel engine journal October 2014
Plant coexistence alters terpene emission and content of Mediterranean species journal March 2007
Origin identification of Pinus nigra populations in southwestern Europe using terpene composition variations journal February 2005
Diterpene resin acids in conifers journal November 2006
Molecular characterization of the pyrolysis of biomass journal March 1987
GC/MS of terpenes in walnut-tree leaves after accelerated solvent extraction journal January 2008
Solvents from Pine journal October 1943
High-Throughput Screening of Plant Cell-Wall Composition Using Pyrolysis Molecular Beam Mass Spectroscopy book January 2009
Methyl Jasmonate Induces Traumatic Resin Ducts, Terpenoid Resin Biosynthesis, and Terpenoid Accumulation in Developing Xylem of Norway Spruce Stems journal July 2002
Needle terpene concentrations and emissions of two coexisting subspecies of Scots pine attacked by the pine processionary moth (Thaumetopoea pityocampa) journal July 2013
Oleoresin tapping of planted slash pine ( Pinus elliottii Engelm. var. elliottii ) adds value and management flexibility to landowners in the southern United States journal September 2014
Catalytic Conversion of Terpenes into Fine Chemicals journal February 2004
Identification and microbial production of a terpene-based advanced biofuel journal September 2011
Simultaneous analysis of monoterpenes and diterpenoids of conifer oleoresin journal September 1993
Induction of isoprenyl diphosphate synthases, plant hormones and defense signalling genes correlates with traumatic resin duct formation in Norway spruce (Picea abies) journal October 2011
Seasonal and geographical variation of terpenes, resin acids and total phenolics in nursery grown seedlings of Scots pine (Pinus sylvestris L.) journal December 1994
The influence of Ceratocystis polonica inoculation and methyl jasmonate application on terpene chemistry of Norway spruce, Picea abies journal August 2010
High-Density Renewable Fuels Based on the Selective Dimerization of Pinenes journal January 2010
Combustion and emissions characterization of terpenes with a view to their biological production in cyanobacteria journal September 2013
Volatile Monoterpenes in Black Currant (Ribes nigrum L.) Juice:  Effects of Heating and Enzymatic Treatment by β-Glucosidase journal February 2006
Pine oleoresin: tapping green chemicals, biofuels, food protection, and carbon sequestration from multipurpose trees
  • Rodrigues-Corrêa, Kelly Cristine da Silva; de Lima, Júlio César; Fett-Neto, Arthur Germano
  • Food and Energy Security, Vol. 1, Issue 2 https://doi.org/10.1002/fes3.13
journal November 2012

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Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANAYLYTICAL CHEMISTRY
fast-GC
pine
pyrolysis-molecular beam mass spectrometry
cell wall chemistry
biofuels