Thermoresponsive PNIPAM Coatings on Nanostructured Gratings for Cell Alignment and Release

Zhernenkov, Mikhail; Ashkar, Rana; Feng, Hao; Akintewe, Olukemi O.; Gallant, Nathan D.; Toomey, Ryan; Ankner, John F.; Pynn, Roger

doi:10.1021/acsami.5b01453

Title: Thermoresponsive PNIPAM Coatings on Nanostructured Gratings for Cell Alignment and Release

Journal Article · Wed May 20 00:00:00 EDT 2015 · ACS Applied Materials and Interfaces

DOI:https://doi.org/10.1021/acsami.5b01453· OSTI ID:1237172

Zhernenkov, Mikhail ^[1]; Ashkar, Rana ^[2]; Feng, Hao ^[3]; Akintewe, Olukemi O. ^[4]; Gallant, Nathan D. ^[4]; Toomey, Ryan ^[4]; Ankner, John F. ^[5]; Pynn, Roger ^[6]

Brookhaven National Lab. (BNL), Upton, NY (United States)
Univ. of Maryland, College Park, MD (United States)
Indiana Univ., Bloomington, IN (United States)
Univ. of South Florida, Tampa, FL (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Indiana Univ., Bloomington, IN (United States). Center for the Exploration of Energy and Matter and Physics Dept.; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Neutron Sciences Directorate

Thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) has been widely used as a surface coating to thermally control the detachment of adsorbed cells without the need for extreme stimuli such as enzyme treatment. Recently, the use of 2D and 3D scaffolds in controlling cell positioning, growth, spreading, and migration has been of a great interest in tissue engineering and cell biology. We use a PNIPAM polymer surface coating atop a nanostructured linear diffraction grating to controllably change the surface topography of 2D linear structures using temperature stimuli. Neutron reflectometry and surface diffraction are utilized to examine the conformity of the polymer coating to the grating surface, its hydration profile, and its evolution in response to temperature variations. Our results show that, in the collapsed state, the PNIPAM coating conforms to the grating structures and retains a uniform hydration of 63%. In the swollen state, the polymer expands beyond the grating channels and absorbs up to 87% water. Such properties are particularly desirable for 2D cell growth scaffolds with a built-in nonextreme tissue-release mechanism. Indeed, the current system demonstrates advanced performance in the effective alignment of cultured fibroblast cells and the easy release of the cells upon temperature change.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0012704; AC05-00OR22725

OSTI ID:: 1237172

Alternate ID(s):: OSTI ID: 1426595

Report Number(s):: BNL-108299-2015-JA; R&D Project: LS001

Journal Information:: ACS Applied Materials and Interfaces, Vol. 7, Issue 22; ISSN 1944-8244

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 22 works

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A review on thermoresponsive cell culture systems based on poly( N -isopropylacrylamide) and derivatives Li, Jiaxing; Fan, Xiaoguang; Yang, Lei International Journal of Polymeric Materials and Polymeric Biomaterials, Vol. 67, Issue 6 https://doi.org/10.1080/00914037.2017.1327436	journal	July 2017
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