skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Thermophilic and thermoacidophilic glycosylation genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods

Patent ·
OSTI ID:1234645

Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for glycosylating and/or post-translationally modifying proteins using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.

Research Organization:
Idaho National Laboratory (INL), Idaho Falls, ID (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC07-99ID13727
Assignee:
BATTELLE ENERGY ALLIANCE, LLC
Patent Number(s):
9,234,228
Application Number:
12/380,450
OSTI ID:
1234645
Resource Relation:
Patent File Date: 2009 Feb 26
Country of Publication:
United States
Language:
English

References (119)

Deciphering the message in protein sequences: tolerance to amino acid substitutions journal March 1990
Powers and Pitfalls in Sequence Analysis: The 70% Hurdle journal April 2000
Maltose and Maltodextrin Transport in the Thermoacidophilic Gram-Positive Bacterium Alicyclobacillus acidocaldarius Is Mediated by a High-Affinity Transport System That Includes a Maltose Binding Protein Tolerant to Low pH journal November 2000
Cloning and transcriptional analysis of the Thermoanaerobacter ethanolicus strain 39E maltose ABC transport system journal March 2002
X-ray Structures of the Maltose–Maltodextrin-binding Protein of the Thermoacidophilic Bacterium Alicyclobacillus acidocaldarius Provide Insight into Acid Stability of Proteins journal January 2004
Functional reconstitution of a maltose ATP-binding cassette transporter from the thermoacidophilic gram-positive bacterium Alicyclobacillus acidocaldarius journal May 2004
Isolation and characterization of a new family 42 β-galactosidase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius: Identification of the active site residues journal February 2008
Thermoacidophilic Microorganisms and their Novel Biocatalysts journal December 2004
Yeast systems for the expression of heterologous gene products journal October 1993
Single molecule detection of RNA reporter probes by amplification with Qβ replicase journal August 1996
Synthesis of an amplifiable reporter RNA for bioassays journal January 1986
Current applications of COS cell based transient expression systems journal October 1993
Manufacture of Xylose-Based Fermentation Media from Corncobs by Posthydrolysis of Autohydrolysis Liquors journal January 2001
Ethanol from lignocellulosic biomass techno-economic performance in short-, middle- and long-term journal April 2005
Immunogenicity and protective efficacy of a tuberculosis DNA vaccine journal August 1996
Biochemistry and genetics of microbial xylanases journal June 1996
Continuous cultures of fused cells secreting antibody of predefined specificity journal August 1975
The acid hydrolysis of sugarcane bagasse hemicellulose to produce xylose, arabinose, glucose and other products journal November 2002
The Effect of Flow Rate of Compressed Hot Water on Xylan, Lignin, and Total Mass Removal from Corn Stover journal September 2003
Baculovirus systems for the expression of human gene products journal October 1993
Microbial Cellulose Utilization: Fundamentals and Biotechnology journal September 2002
Lignocellulose biodegradation: Fundamentals and applications journal June 2002
Analytical strategies for the use of DNA probes journal February 1988
The Synthesis of Bovine Insulin by the Solid Phase Method journal November 1966
Autocatalytic replication of a recombinant RNA journal December 1983
A general method applicable to the search for similarities in the amino acid sequence of two proteins journal March 1970
Recent advances in heterologous gene expression in Escherichia coli journal October 1993
Prokaryotic glycosylation journal February 2001
Strand displacement amplification—an isothermal, in vitro DNA amplification technique journal January 1992
Microbial β-Glucosidases: Cloning, Properties, and Applications journal January 2002
Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids journal November 1998
Practical limits of function prediction journal January 2000
Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different journal April 2001
Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine journal September 1999
Xylanases, xylanase families and extremophilic xylanases journal January 2005
Purification and Properties of Acid Stable Xylanases from Aspergillus kawachii journal January 1992
Microbial hemicellulases journal June 2003
Gene cloning, sequencing, and characterization of a family 9 endoglucanase (CelA) with an unusual pattern of activity from the thermoacidophile Alicyclobacillus acidocaldarius ATCC27009 journal December 2002
A new class of biodegradable materials: Poly-3-hydroxy-butyrate/steam exploded straw fiber composites. I. Thermal and impact behavior journal September 1993
Interlaboratory testing of methods for assay of xylanase activity journal May 1992
Molecular diversity of thermophilic cellulolytic and hemicellulolytic bacteria journal February 1999
Glycosidases and glycosyl transferases in glycoside and oligosaccharide synthesis journal February 1998
The nature of lignocellulosics and their pretreatments for enzymatic hydrolysis book January 1982
Development of gas phase bioreactors for the removal of nitrogen oxides from synthetic flue gas streams journal October 2002
Crystallographic and mutational analyses of an extremely acidophilic and acid-stable xylanase: biased distribution of acidic residues and importance of Asp37 for catalysis at low pH journal December 1998
Transformation of Acidiphilium by electroporation and conjugation journal May 1992
Pretreatment for enhanced hydrolysis of cellulosic biomass journal January 1984
Comparison of the economics of acid and enzymatic hydrolysis of newsprint journal April 1978
Lignochemicals journal February 1982
Fungal Upgrading of Wheat Straw for Straw-Thermoplastics Production journal January 2004
Microbial Pretreatment of Biomass: Potential for Reducing Severity of Thermochemical Biomass Pretreatment journal January 2003
journal October 2003
Partial acid hydrolysis of cellulosic materials as a pretreatment for enzymatic hydrolysis journal July 1980
Molecular and biotechnological aspects of xylanases journal July 1999
Characterization of a β-glycosidase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius journal April 2006
Oxygen effects on thermophilic microbial populations in biofilters treating nitric oxide containing off-gas streams journal October 2001
Overview and Evaluation of Fuel Ethanol from Cellulosic Biomass: Technology, Economics, the Environment, and Policy journal November 1996
Specificity of the protein secretory apparatus: secretion of the heat-labile enterotoxin B subunit pentamers by different species of Gram- bacteria journal January 1995
Industrial Scale-Up of pH-Controlled Liquid Hot Water Pretreatment of Corn Fiber for Fuel Ethanol Production journal January 2005
Purification and characterization of an acidophilic xylanase from Aureobasidium pullulans var. melanigenum and sequence analysis of the encoding gene journal January 2001
Simultaneous saccharification and fermentation of cellulose: Effect of ethanol on enzymatic saccharification of cellulose journal April 1985
Generation of transposon insertion mutant libraries for Gram-positive bacteria by electroporation of phage Mu DNA transposition complexes journal April 2005
Biotransformation of Toxic Organic and Inorganic Contaminants by Halophilic Bacteria book January 2004
The Path Forward for Biofuels and Biomaterials journal January 2006
Biomechanical and Biochemical Pulping of Sugarcane Bagasse with Ceriporiopsis subvermispora Fungal and Xylanase Pretreatments journal March 2001
Quantitative Saccharification of Wood and Cellulose journal January 1945
Dilute Acid Pretreatment, Enzymatic Saccharification, and Fermentation of Rice Hulls to Ethanol journal January 2005
Rapid production of thermostable cellulase-free xylanase by a strain of Bacillus subtilis and its properties journal June 2002
Dilute-Sulfuric Acid Pretreatment of Corn Stover in Pilot-Scale Reactor book January 2003
An extremely thermostable xylanase from the thermophilic eubacterium Thermotoga journal July 1991
Cellulase-free xylanases from Bacillus and other microorganisms journal February 2000
Glycosyl hydrolases from hyperthermophiles journal February 1997
Production of a cellulase-free xylanase from agricultural waste materials by a thermotolerant Streptomyces sp. journal October 2001
Comparison of pretreatment methods on the basis of available surface area journal January 1992
In vitro degradation of natural insoluble lignin in aqueous media by the extracellular peroxidases of Phanerochaete chrysosporium journal March 1998
Measurement of fumonisins in corn with a fiber optic fluoroimmunosensor conference May 1997
Preliminary Investigation of Fungal Bioprocessing of Wheat Straw for Production of Straw-Thermoplastic Composites book January 2003
Purification and Characterization of a Novel Thermo-Alkali-Stable Catalase from Thermus brockianus journal January 2003
Potential and utilization of thermophiles and thermostable enzymes in biorefining journal January 2007
The first description of an archaeal hemicellulase: the xylanase from Thermococcus zilligii strain AN1 journal November 1999
Xylanases in bleaching: From an idea to the industry journal March 1994
Characterization of a new bacteriophage which infects bacteria of the genus Acidiphilium journal November 1993
Electrotransformation of Acidophilic, Heterotrophic, Gram-negative Bacteria book January 2000
PCR ligation mutagenesis in transformable streptococci: application and efficiency journal April 2002
Purification, Properties and Structural Aspects of a Thermoacidophilic alpha-Amylase from Alicyclobacillus Acidocaldarius Atcc 27009 journal December 1994
Bacterial glycoproteins: Functions, biosynthesis and applications journal April 2003
Process for pretreating cellulosic substrates and for producing sugar therefrom patent December 1980
Method of detecting and characterizing a nucleic acid or reactant for the application of this method patent April 1986
Plasmid, method for construction of the same, microorganism carrying the plasmid and method for cultivation of the microorganism patent November 1986
Probe containing a modified nucleic acid recognizable by specific antibodies and use of this probe to detect and characterize a homologous dna sequence patent March 1992
Thermostable .alpha.-L-arabinofuranosidase from Aureobasidium pullulans patent March 1999
Thermostable xylanase patent June 1999
Xylanase obtained from an anaerobic fungus patent September 1999
Thermostable xylanases patent July 2000
Xyloglucan-specific alkaline xyloglucanase from bacillus patent July 2001
Xylanase derived from a Bacillus species, expression vectors for such xylanase and other proteins, host organisms therefor and use thereof patent July 2002
Polypeptides having haloperoxidase activity patent January 2003
Microbial process for producing L-ascorbic acid, D-erythorbic acid, and salts thereof patent August 2004
‘Pseudomonas stutzeri’ strain and process for preparation of xylanase patent December 2004
Enzyme and methodology for the treatment of a biomass patent June 2010
Process for hydrolyzing starch without pH adjustment patent-application July 2003
Tomato plants that exhibit resistance to botrytis cinerea patent-application December 2003
Expression of microbial proteins in plants for production of plants with improved properties patent-application December 2003
Identification of essential genes in microorganisms patent-application February 2004
High temperature and alkaline stable catalase patent-application May 2005
Promoters and proteins from Clostridium thermocellum and uses thereof patent-application May 2006
Products and processes for in vitro synthesis of biomolecules patent-application September 2006
Polypeptides of Alicyclobacillus sp. patent-application April 2007
Compositions and methods for the synthesis and subsequent modification of uridine-5'-diphosphosulfoquinovose (UDP-SQ) patent-application June 2007
Synthesis of oligosaccharides, glycolipds, and glycoproteins using bacterial glycosyltransferases patent-application June 2007
Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods patent-application August 2009
Transcriptional control in alicyclobacillus acidocaldarius and associated genes, proteins, and methods patent-application August 2009
Thermophilic Organisms For Conversion Of Lignocellulosic Biomass To Ethanol patent-application September 2009
Thermal and acid tolerant beta-xylosidases, genes encoding, related organisms, and methods patent-application September 2009
Thermophilic and thermoacidophilic sugar transporter genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods patent-application October 2009
Thermophilic and thermoacidophilic metabolism genes and enzymes from alicyclobacillus acidocaldadarius and related organisms, methods patent-application October 2009
Alteration and modulation of protein activity by varying post-translational modification patent-application August 2010
Thermal and acid tolerant beta xylosidases, arabinofuranosidases, genes encoding, related organisms, and methods patent-application December 2010
Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes patent-application April 2011