Enhanced citric acid production in aspergillus with inactivated asparagine-linked glycosylation protein 3 (ALG3), and/or increased laeA expression
Abstract
Provided herein are fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (Lae), or both. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also provided, as are compositions and kits including the disclosed fungi.
- Inventors:
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1228371
- Patent Number(s):
- 9,206,450
- Application Number:
- 14/703,499
- Assignee:
- Battelle Memorial Institute
- DOE Contract Number:
- AC05-76RL01830
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2015 May 04
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Dai, Ziyu, and Baker, Scott E. Enhanced citric acid production in aspergillus with inactivated asparagine-linked glycosylation protein 3 (ALG3), and/or increased laeA expression. United States: N. p., 2015.
Web.
Dai, Ziyu, & Baker, Scott E. Enhanced citric acid production in aspergillus with inactivated asparagine-linked glycosylation protein 3 (ALG3), and/or increased laeA expression. United States.
Dai, Ziyu, and Baker, Scott E. 2015.
"Enhanced citric acid production in aspergillus with inactivated asparagine-linked glycosylation protein 3 (ALG3), and/or increased laeA expression". United States. https://www.osti.gov/servlets/purl/1228371.
@article{osti_1228371,
title = {Enhanced citric acid production in aspergillus with inactivated asparagine-linked glycosylation protein 3 (ALG3), and/or increased laeA expression},
author = {Dai, Ziyu and Baker, Scott E.},
abstractNote = {Provided herein are fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (Lae), or both. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also provided, as are compositions and kits including the disclosed fungi.},
doi = {},
url = {https://www.osti.gov/biblio/1228371},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Dec 08 00:00:00 EST 2015},
month = {Tue Dec 08 00:00:00 EST 2015}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Process for producing of citric acid and monovalent citrate salts
patent, July 1996
- Datta, Rathin; Bergemann, Eugene P.
- US Patent Document 5,532,148
Global regulator of secondary metabolite biosynthesis and methods of use
patent-application, March 2004
- Keller, Nancy P.; Bok, Jin Woo
- US Patent Application 10/668696; 20040058872
Novel Hansenula Polymorpha Gene Coding for Dolichyl-Phosphate- Mannose Dependent Alpha-1,3 Mannosyltransferase and Process for the Production of Recombinant Glycoproteins With Hansenula Polymorpha Mutant Strain Deficient in
patent-application, March 2010
- Kang, Hyun Ah; Park, Jeong Seok; Kim, Moo Woong
- US Patent Application 12/091217; 20100062485
Cloning and characterization of the ALG3 gene of Saccharomyces cerevisiae
journal, January 1996
- Aebi, Markus; Gassenhuber, Johann; Domdey, Horst
- Glycobiology, Vol. 6, Issue 4, p. 439-444
Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015 versus enzyme-producing CBS 513.88
journal, May 2011
- Andersen, M. R.; Salazar, M. P.; Schaap, P. J.
- Genome Research, Vol. 21, Issue 6, p. 885-897
On the frequency of protein glycosylation, as deduced from analysis of the SWISS-PROT database
journal, December 1999
- Apweiler, Rolf; Hermjakob, Henning; Sharon, Nathan
- Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1473, Issue 1, p. 4-8
Identification and characterization of Penicillium citrinum VeA and LaeA as global regulators for ML-236B production
journal, December 2011
- Baba, Satoshi; Kinoshita, Hiroshi; Nihira, Takuya
- Current Genetics, Vol. 58, Issue 1, p. 1-11
VelB/VeA/LaeA Complex Coordinates Light Signal with Fungal Development and Secondary Metabolism
journal, June 2008
- Bayram, O.; Krappmann, S.; Ni, M.
- Science, Vol. 320, Issue 5882, p. 1504-1506
LaeA Control of Velvet Family Regulatory Proteins for Light-Dependent Development and Fungal Cell-Type Specificity
journal, December 2010
- Sarikaya Bayram, Özlem; Bayram, Özgür; Valerius, Oliver
- PLoS Genetics, Vol. 6, Issue 12, Article No. e1001226
LaeA, a Regulator of Secondary Metabolism in Aspergillus spp.
journal, April 2004
- Bok, Jin Woo; Keller, Nancy P.
- Eukaryotic Cell, Vol. 3, Issue 2, p. 527-535
Secondary metabolic gene cluster silencing in Aspergillus nidulans
journal, September 2006
- Bok, Jin Woo; Noordermeer, Daan; Kale, Shubha P.
- Molecular Microbiology, Vol. 61, Issue 6, p. 1636-1645
Chromatin-level regulation of biosynthetic gene clusters
journal, May 2009
- Bok, Jin Woo; Chiang, Yi-Ming; Szewczyk, Edyta
- Nature Chemical Biology, Vol. 5, Issue 7, p. 462-464
Accurate prediction of the Aspergillus nidulans terrequinone gene cluster boundaries using the transcriptional regulator LaeA
journal, November 2007
- Bouhired, Sarah; Weber, Monika; Kempf-Sontag, Anita
- Fungal Genetics and Biology, Vol. 44, Issue 11, p. 1134-1145
Mutational Analysis of the Glycosylphosphatidylinositol (GPI) Anchor Pathway Demonstrates that GPI-Anchored Proteins Are Required for Cell Wall Biogenesis and Normal Hyphal Growth in Neurospora crassa
journal, March 2006
- Bowman, Shaun M.; Piwowar, Amy; Al Dabbous, Mash'el
- Eukaryotic Cell, Vol. 5, Issue 3, p. 587-600
Lae1 regulates expression of multiple secondary metabolite gene clusters in Fusarium verticillioides
journal, August 2012
- Butchko, Robert A. E.; Brown, Daren W.; Busman, Mark
- Fungal Genetics and Biology, Vol. 49, Issue 8, p. 602-612
The VeA regulatory system and its role in morphological and chemical development in fungi
journal, July 2008
- Calvo, Ana M.
- Fungal Genetics and Biology, Vol. 45, Issue 7, p. 1053-1061
Functional analysis of the ALG3 gene encoding the Dol-P-Man: Man5GlcNAc2-PP-Dol mannosyltransferase enzyme of P. pastoris
journal, January 2004
- Davidson, Robert C.; Nett, Juergen H.; Renfer, Eduard
- Glycobiology, Vol. 14, Issue 5, p. 399-407
A plant DNA minipreparation: Version II
journal, September 1983
- Dellaporta, Stephen L.; Wood, Jonathan; Hicks, James B.
- Plant Molecular Biology Reporter, Vol. 1, Issue 4, p. 19-21
Congenital Disorder of Glycosylation Type Id: Clinical Phenotype, Molecular Analysis, Prenatal Diagnosis, and Glycosylation of Fetal Proteins
journal, August 2005
- Denecke, Jonas; Kranz, Christian; Von Kleist-Retzow, Juergen Ch.
- Pediatric Research, Vol. 58, Issue 2, p. 248-253
Proteomics of industrial fungi: trends and insights for biotechnology
journal, October 2010
- de Oliveira, José Miguel P. Ferreira; de Graaff, Leo H.
- Applied Microbiology and Biotechnology, Vol. 89, Issue 2, p. 225-237
Protein glycosylation pathways in filamentous fungi
journal, May 2008
- Deshpande, Nandan; Wilkins, Marc R.; Packer, Nicolle
- Glycobiology, Vol. 18, Issue 8, p. 626-637
Beyond aflatoxin: four distinct expression patterns and functional roles associated with Aspergillus flavus secondary metabolism gene clusters
journal, March 2010
- Georgianna, D. Ryan; Fedorova, Natalie D.; Burroughs, James L.
- Molecular Plant Pathology, Vol. 11, Issue 2, p. 213-226
Advances in the production of human therapeutic proteins in yeasts and filamentous fungi
journal, November 2004
- Gerngross, Tillman U.
- Nature Biotechnology, Vol. 22, Issue 11, p. 1409-1414
Genomics of protein folding in the endoplasmic reticulum, secretion stress and glycosylation in the aspergilli
journal, March 2009
- Geysens, Steven; Whyteside, Graham; Archer, David B.
- Fungal Genetics and Biology, Vol. 46, Issue 1, p. S121-S140
Role of Glycosylation in Development
journal, June 2004
- Haltiwanger, Robert S.; Lowe, John B.
- Annual Review of Biochemistry, Vol. 73, Issue 1, p. 491-537
Effective lead selection for improved protein production in Aspergillus niger based on integrated genomics
journal, March 2009
- Jacobs, Denise I.; Olsthoorn, Maurien M. A.; Maillet, Isabelle
- Fungal Genetics and Biology, Vol. 46, Issue 1, p. S141-S152
N-Glycan Modification in Aspergillus Species
journal, December 2007
- Kainz, E.; Gallmetzer, A.; Hatzl, C.
- Applied and Environmental Microbiology, Vol. 74, Issue 4, p. 1076-1086
Requirement of LaeA for secondary metabolism and sclerotial production in Aspergillus flavus
journal, October 2008
- Kale, Shubha P.; Milde, Lane; Trapp, Marisa K.
- Fungal Genetics and Biology, Vol. 45, Issue 10, p. 1422-1429
Arabidopsis thaliana ALG3 mutant synthesizes immature oligosaccharides in the ER and accumulates unique N-glycans
journal, March 2010
- Kajiura, H.; Seki, T.; Fujiyama, K.
- Glycobiology, Vol. 20, Issue 6, p. 736-751
LaeA, a global regulator of Aspergillus toxins
journal, January 2006
- Keller, Nancy; Bok, Jinwoo; Chung, Dawoon
- Medical Mycology, Vol. 44, Issue s1, p. 83-85
Centralized Modularity of N-Linked Glycosylation Pathways in Mammalian Cells
journal, October 2009
- Kim, Pan-Jun; Lee, Dong-Yup; Jeong, Hawoong
- PLoS ONE, Vol. 4, Issue 10, Article No. e7317
Carbohydrate deficient glycoprotein syndrome type IV: deficiency of dolichyl‐P‐Man:Man5 GlcNAc2‐PP‐dolichyl mannosyltransferase
journal, December 1999
- Körner, Christian; Knauer, Roland; Stephani, Ulrich
- The EMBO Journal, Vol. 18, Issue 23, p. 6816-6822
Assembly of Asparagine-Linked Oligosaccharides
journal, June 1985
- Kornfeld, R.; Kornfeld, S.
- Annual Review of Biochemistry, Vol. 54, Issue 1, p. 631-664
Approaching the Secrets of N-Glycosylation in Aspergillus fumigatus: Characterization of the AfOch1 Protein
journal, December 2010
- Kotz, Andrea; Wagener, Johannes; Engel, Jakob
- PLoS ONE, Vol. 5, Issue 12, Article No. e15729
CDG-Id in two siblings with partially different phenotypes
journal, January 2007
- Kranz, Christian; Sun, Liangwu; Eklund, Erik A.
- American Journal of Medical Genetics Part A, Vol. 143A, Issue 13, p. 1414-1420
Protein Glycosylation in Yeast
journal, June 1987
- Kukuruzinska, M. A.; Bergh, M. L. E.; Jackson, B. J.
- Annual Review of Biochemistry, Vol. 56, Issue 1, p. 915-944
α-1,6-Mannosylation of N-Linked Oligosaccharide Present on Cell Wall Proteins Is Required for Their Incorporation into the Cell Wall in the Filamentous Fungus Neurospora crassa
journal, September 2010
- Maddi, Abhiram; Free, Stephen J.
- Eukaryotic Cell, Vol. 9, Issue 11, p. 1766-1775
Deletion of the TbALG3 gene demonstrates site-specific N-glycosylation and N-glycan processing in Trypanosoma brucei
journal, February 2008
- Manthri, Sujatha; Güther, M. Lucia S.; Izquierdo, Luis
- Glycobiology, Vol. 18, Issue 5, p. 367-383
Molecular cloning and enzymatic characterization of a Trichoderma reesei 1,2-α-d-mannosidase
journal, February 2000
- Maras, Marleen; Callewaert, Nico; Piens, Kathleen
- Journal of Biotechnology, Vol. 77, Issue 2-3, p. 255-263
The effects of culture conditions on the glycosylation of secreted human placental alkaline phosphatase produced in Chinese hamster ovary cells
journal, August 2008
- Nam, Jong Hyun; Zhang, Fuming; Ermonval, Myriam
- Biotechnology and Bioengineering, Vol. 100, Issue 6, p. 1178-1192
Heterologous protein expression in filamentous fungi
journal, September 2005
- Nevalainen, K. M. Helena; Te'o, Valentino S. J.; Bergquist, Peter L.
- Trends in Biotechnology, Vol. 23, Issue 9, p. 468-474
Aspergillus oryzae laeA Regulates Kojic Acid Synthesis Genes
journal, September 2011
- Oda, Ken; Kobayashi, Akiko; Ohashi, Shinichi
- Bioscience, Biotechnology, and Biochemistry, Vol. 75, Issue 9, p. 1832-1834
Human Sperm Binding Is Mediated by the Sialyl-Lewisx Oligosaccharide on the Zona Pellucida
journal, August 2011
- Pang, P.-C.; Chiu, P. C. N.; Lee, C.-L.
- Science, Vol. 333, Issue 6050, p. 1761-1764
Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88
journal, January 2007
- Pel, Herman J.; de Winde, Johannes H.; Archer, David B.
- Nature Biotechnology, Vol. 25, Issue 2, p. 221-231
veA-dependent RNA-pol II transcription elongation factor-like protein, RtfA, is associated with secondary metabolism and morphological development in Aspergillus nidulans
journal, July 2012
- Ramamoorthy, Vellaisamy; Shantappa, Sourabha; Dhingra, Sourabh
- Molecular Microbiology, Vol. 85, Issue 4, p. 795-814
Heterochromatic marks are associated with the repression of secondary metabolism clusters in Aspergillus nidulans
journal, February 2010
- Reyes-Dominguez, Yazmid; Bok, Jin Woo; Berger, Harald
- Molecular Microbiology, Vol. 76, Issue 6, p. 1376-1386
Volatile profiling reveals intracellular metabolic changes in Aspergillus parasiticus: veA regulates branched chain amino acid and ethanol metabolism
journal, January 2010
- Roze, Ludmila V.; Chanda, Anindya; Laivenieks, Maris
- BMC Biochemistry, Vol. 11, Issue 1, Article No. 33
Heterologous expression system in Aspergillus oryzae for fungal biosynthetic gene clusters of secondary metabolites
journal, November 2011
- Sakai, Kanae; Kinoshita, Hiroshi; Nihira, Takuya
- Applied Microbiology and Biotechnology, Vol. 93, Issue 5, p. 2011-2022
Microbial production of organic acids: expanding the markets
journal, February 2008
- Sauer, Michael; Porro, Danilo; Mattanovich, Diethard
- Trends in Biotechnology, Vol. 26, Issue 2, p. 100-108
Organic Acid Production by Filamentous Fungi
book, January 2004
- Magnuson, Jon K.; Lasure, Linda L.; Tkacz, Jan S.
- Advances in Fungal Biotechnology for Industry, Agriculture, and Medicine, p. 307-340
CDG-Id caused by homozygosity for an ALG3 mutation due to segmental maternal isodisomy UPD3(q21.3-qter)
journal, April 2005
- Schollen, E.; Grünewald, S.; Keldermans, L.
- European Journal of Medical Genetics, Vol. 48, Issue 2, p. 153-158
On the safety of Aspergillus niger - a review
journal, January 2002
- Schuster, E.; Dunn-Coleman, N.; Frisvad, J.
- Applied Microbiology and Biotechnology, Vol. 59, Issue 4-5, p. 426-435
Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different
journal, April 2001
- Seffernick, J. L.; de Souza, M. L.; Sadowsky, M. J.
- Journal of Bacteriology, Vol. 183, Issue 8, p. 2405-2410
Overexpression of the Aspergillus nidulans histone 4 acetyltransferase EsaA increases activation of secondary metabolite production
journal, August 2012
- Soukup, Alexandra A.; Chiang, Yi-Ming; Bok, Jin Woo
- Molecular Microbiology, Vol. 86, Issue 2, p. 314-330
The ARO4 gene of Candida albicans encodes a tyrosine-sensitive DAHP synthase: evolution, functional conservation and phenotype of Aro3p-, Aro4p-deficient mutants
journal, May 2002
- Livi, George P.; McLaughlin, Megan M.; Sousa, Silvino
- Microbiology, Vol. 148, Issue 5, p. 1291-1303
Carbohydrate-Deficient Glycoprotein Syndrome - A Fourth Subtype
journal, October 1995
- Stibler, Helena; Stephani, U.; Kutsch, Ulrike
- Neuropediatrics, Vol. 26, Issue 05, p. 235-237
Congenital Disorder of Glycosylation Id Presenting with Hyperinsulinemic Hypoglycemia and Islet Cell Hyperplasia
journal, July 2005
- Sun, Liangwu; Eklund, Erik A.; Chung, Wendy K.
- The Journal of Clinical Endocrinology & Metabolism, Vol. 90, Issue 7, p. 4371-4375
Quality Control and Protein Folding in the Secretory Pathway
journal, November 2003
- Trombetta, E. Sergio; Parodi, Armando J.
- Annual Review of Cell and Developmental Biology, Vol. 19, Issue 1, p. 649-676
Analytical and computational approaches to define the Aspergillus niger secretome
journal, March 2009
- Tsang, Adrian; Butler, Gregory; Powlowski, Justin
- Fungal Genetics and Biology, Vol. 46, Issue 1, p. S153-S160
Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine
journal, September 1999
- Witkowski, Andrzej; Joshi, Anil K.; Lindqvist, Ylva
- Biochemistry, Vol. 38, Issue 36, p. 11643-11650
Molecular cloning and characterization of the global regulator LaeA in Penicillium citrinum
journal, August 2010
- Xing, Wei; Deng, Chao; Hu, Chang-Hua
- Biotechnology Letters, Vol. 32, Issue 11, p. 1733-1737
Unraveling the Mechanism of Protein N-Glycosylation
journal, December 2004
- Yan, Aixin; Lennarz, William J.
- Journal of Biological Chemistry, Vol. 280, Issue 5, p. 3121-3124
Global analysis of gene transcription regulation in prokaryotes
journal, August 2006
- Zhou, D.; Yang, R.
- Cellular and Molecular Life Sciences, Vol. 63, Issue 19-20, p. 2260-2290