Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing strains of Synechococcus sp. PCC 7002

Therien, Jesse B.; Zadvornyy, Oleg A.; Posewitz, Matthew C.; Bryant, Donald A.; Peters, John W.

doi:10.1186/s13068-014-0154-2

Title: Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing strains of Synechococcus sp. PCC 7002

Journal Article · Sat Oct 18 00:00:00 EDT 2014 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-014-0154-2· OSTI ID:1221648

Therien, Jesse B. ^[1]; Zadvornyy, Oleg A. ^[1]; Posewitz, Matthew C. ^[2]; Bryant, Donald A. ^[3]; Peters, John W. ^[1]

Montana State Univ., Bozeman, MT (United States)
Colorado School of Mines, Golden, CO (United States)
Montana State Univ., Bozeman, MT (United States); The Pennsylvania State Univ., University Park, PA (United States)

The model alga Chlamydomonas reinhardtii requires acetate as a co-substrate for optimal production of lipids, and the addition of acetate to culture media has practical and economic implications for algal biofuel production. We demonstrate the growth of C. reinhardtii on acetate provided by mutant strains of the cyanobacterium Synechococcus sp. PCC7002.

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Research Organization:: Montana State Univ., Bozeman, MT (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE)

Grant/Contract Number:: EE0003136

OSTI ID:: 1221648

Journal Information:: Biotechnology for Biofuels, Vol. 7, Issue 1; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 23 works

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References (22)

Genetic engineering of fatty acid chain length in Phaeodactylum tricornutum Radakovits, Randor; Eduafo, Patrick M.; Posewitz, Matthew C. Metabolic Engineering, Vol. 13, Issue 1 https://doi.org/10.1016/j.ymben.2010.10.003	journal	January 2011
Biogenic hydrogen and methane production from Chlorella vulgaris and Dunaliella tertiolecta biomass Lakaniemi, Aino-Maija; Hulatt, Christopher J.; Thomas, David N. Biotechnology for Biofuels, Vol. 4, Issue 1 https://doi.org/10.1186/1754-6834-4-34	journal	January 2011
Co-culturing a novel Bacillus strain with Clostridium tyrobutyricum ATCC 25755 to produce butyric acid from sucrose Dwidar, Mohammed; Kim, Seil; Jeon, Byoung Seung Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-35	journal	January 2013
Biofuels from algae: challenges and potential Hannon, Michael; Gimpel, Javier; Tran, Miller Biofuels, Vol. 1, Issue 5 https://doi.org/10.4155/bfs.10.44	journal	September 2010
Biodiesel production by simultaneous extraction and conversion of total lipids from microalgae, cyanobacteria, and wild mixed-cultures Wahlen, Bradley D.; Willis, Robert M.; Seefeldt, Lance C. Bioresource Technology, Vol. 102, Issue 3 https://doi.org/10.1016/j.biortech.2010.11.026	journal	February 2011
Synechococcus sp. Strain PCC 7002 Transcriptome: Acclimation to Temperature, Salinity, Oxidative Stress, and Mixotrophic Growth Conditions Ludwig, Marcus; Bryant, Donald A. Frontiers in Microbiology, Vol. 3 https://doi.org/10.3389/fmicb.2012.00354	journal	January 2012
Determination of Unknown Concentrations of Sodium Acetate Using the Method of Standard Addition and Proton NMR: An Experiment for the Undergraduate Analytical Chemistry Laboratory Rajabzadeh, Massy Journal of Chemical Education, Vol. 89, Issue 11 https://doi.org/10.1021/ed200577v	journal	August 2012
Increased Lipid Accumulation in the Chlamydomonas reinhardtii sta7-10 Starchless Isoamylase Mutant and Increased Carbohydrate Synthesis in Complemented Strains Work, Victoria H.; Radakovits, Randor; Jinkerson, Robert E. Eukaryotic Cell, Vol. 9, Issue 8 https://doi.org/10.1128/EC.00075-10	journal	June 2010
Hydrogen production by sulfur-deprived Chlamydomonas reinhardtii under photoautotrophic conditions Tsygankov, A.; Kosourov, S.; Tolstygina, I. International Journal of Hydrogen Energy, Vol. 31, Issue 11 https://doi.org/10.1016/j.ijhydene.2006.06.024	journal	September 2006
Engineering algae for biohydrogen and biofuel production Beer, Laura L.; Boyd, Eric S.; Peters, John W. Current Opinion in Biotechnology, Vol. 20, Issue 3 https://doi.org/10.1016/j.copbio.2009.06.002	journal	June 2009
Regulatory branch points affecting protein and lipid biosynthesis in the diatom Phaeodactylum tricornutum Guerra, L. Tiago; Levitan, Orly; Frada, Miguel J. Biomass and Bioenergy, Vol. 59 https://doi.org/10.1016/j.biombioe.2013.10.007	journal	December 2013
Microalgae and terrestrial biomass as source for fuels—A process view Posten, Clemens; Schaub, Georg Journal of Biotechnology, Vol. 142, Issue 1 https://doi.org/10.1016/j.jbiotec.2009.03.015	journal	June 2009
Demonstration of sustained hydrogen photoproduction by immobilized, sulfur-deprived Chlamydomonas reinhardtii cells Laurinavichene, T.; Fedorov, A.; Ghirardi, M. International Journal of Hydrogen Energy, Vol. 31, Issue 5 https://doi.org/10.1016/j.ijhydene.2005.05.002	journal	April 2006
Altered carbohydrate metabolism in glycogen synthase mutants of Synechococcus sp. strain PCC 7002: Cell factories for soluble sugars Xu, Yu; Tiago Guerra, L.; Li, Zhongkui Metabolic Engineering, Vol. 16 https://doi.org/10.1016/j.ymben.2012.12.002	journal	March 2013
Second Generation Biofuels: High-Efficiency Microalgae for Biodiesel Production Schenk, Peer M.; Thomas-Hall, Skye R.; Stephens, Evan BioEnergy Research, Vol. 1, Issue 1 https://doi.org/10.1007/s12155-008-9008-8	journal	March 2008
Starchless Mutants of Chlamydomonas reinhardtii Lack the Small Subunit of a Heterotetrameric ADP-Glucose Pyrophosphorylase Zabawinski, C.; Van Den Koornhuyse, N.; D'Hulst, C. Journal of Bacteriology, Vol. 183, Issue 3 https://doi.org/10.1128/JB.183.3.1069-1077.2001	journal	February 2001
Life cycle assessment of biodiesel production from microalgae in ponds Campbell, Peter K.; Beer, Tom; Batten, David Bioresource Technology, Vol. 102, Issue 1 https://doi.org/10.1016/j.biortech.2010.06.048	journal	January 2011
Phenotypic diversity of hydrogen production in chlorophycean algae reflects distinct anaerobic metabolisms Meuser, Jonathan E.; Ananyev, Gennady; Wittig, Lauren E. Journal of Biotechnology, Vol. 142, Issue 1 https://doi.org/10.1016/j.jbiotec.2009.01.015	journal	June 2009
Redirecting Reductant Flux into Hydrogen Production via Metabolic Engineering of Fermentative Carbon Metabolism in a Cyanobacterium McNeely, K.; Xu, Y.; Bennette, N. Applied and Environmental Microbiology, Vol. 76, Issue 15 https://doi.org/10.1128/aem.00862-10	journal	June 2010
NUTRITIONAL STUDIES WITH CHLAMYDOMONAS REINHARDI Sager, Ruth; Granick, S. Annals of the New York Academy of Sciences, Vol. 56, Issue 5 https://doi.org/10.1111/j.1749-6632.1953.tb30261.x	journal	October 1953
A quick isolation method for mutants with high lipid yield in oleaginous yeast Wang, Jufang; Li, Renmin; Lu, Dong World Journal of Microbiology and Biotechnology, Vol. 25, Issue 5 https://doi.org/10.1007/s11274-009-9960-2	journal	January 2009
The Production of Hydrogen Peroxide by Blue-Green Algae: a Survey Stevens, S. E.; Patterson, C. O. Pat; Myers, Jack Journal of Phycology, Vol. 9, Issue 4 https://doi.org/10.1111/j.1529-8817.1973.tb04116.x	journal	December 1973

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Alignment of microbial fitness with engineered product formation: obligatory coupling between acetate production and photoautotrophic growth Du, Wei; Jongbloets, Joeri A.; van Boxtel, Coco Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1037-8	journal	February 2018
Mimicking lichens: incorporation of yeast strains together with sucrose-secreting cyanobacteria improves survival, growth, ROS removal, and lipid production in a stable mutualistic co-culture production platform Li, Tingting; Li, Chien-Ting; Butler, Kirk Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0736-x	journal	March 2017
Interactions Between Microalgae and Microorganisms for Wastewater Remediation and Biofuel Production Hu, Zhan; Qi, Yun; Zhao, Liu Waste and Biomass Valorization, Vol. 10, Issue 12 https://doi.org/10.1007/s12649-018-0325-7	journal	May 2018
Lauric Acid Production in a Glycogen-Less Strain of Synechococcus sp. PCC 7002 Work, Victoria H.; Melnicki, Matthew R.; Hill, Eric A. Frontiers in Bioengineering and Biotechnology, Vol. 3 https://doi.org/10.3389/fbioe.2015.00048	journal	April 2015
Metabolomic and physiological changes of Chlamydomonas reinhardtii (Chlorophyceae, Chlorophyta) during batch culture development Puzanskiy, Roman; Tarakhovskaya, Elena; Shavarda, Alexey Journal of Applied Phycology, Vol. 30, Issue 2 https://doi.org/10.1007/s10811-017-1326-9	journal	November 2017

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59 BASIC BIOLOGICAL SCIENCES
09 BIOMASS FUELS
biofuels
algae
cyanobacteria
lipid production
acetate production
co-culture
alginate immobilization

Title: Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing strains of Synechococcus sp. PCC 7002

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