Evaluation of candidate vaccine approaches for MERS-CoV

Wang, Lingshu; Shi, Wei; Joyce, M. Gordon; Modjarrad, Kayvon; Zhang, Yi; Leung, Kwanyee; Lees, Christopher R.; Zhou, Tongqing; Yassine, Hadi M.; Kanekiyo, Masaru; Yang, Zhi-yong; Chen, Xuejun; Becker, Michelle M.; Freeman, Megan; Vogel, Leatrice; Johnson, Joshua C.; Olinger, Gene; Todd, John P.; Bagci, Ulas; Solomon, Jeffrey; Mollura, Daniel J.; Hensley, Lisa; Jahrling, Peter; Denison, Mark R.; Rao, Srinivas S.; Subbarao, Kanta; Kwong, Peter D.; Mascola, John R.; Kong, Wing-Pui; Graham, Barney S.

doi:10.1038/ncomms8712

Title: Evaluation of candidate vaccine approaches for MERS-CoV

Journal Article · Tue Jul 28 00:00:00 EDT 2015 · Nature Communications

DOI:https://doi.org/10.1038/ncomms8712· OSTI ID:1214709

Wang, Lingshu; Shi, Wei; Joyce, M. Gordon; Modjarrad, Kayvon; Zhang, Yi; Leung, Kwanyee; Lees, Christopher R.; Zhou, Tongqing; Yassine, Hadi M.; Kanekiyo, Masaru; Yang, Zhi-yong; Chen, Xuejun; Becker, Michelle M.; Freeman, Megan; Vogel, Leatrice; Johnson, Joshua C.; Olinger, Gene; Todd, John P.; Bagci, Ulas; Solomon, Jeffrey more »

The emergence of Middle East respiratory syndrome coronavirus (MERS-CoV) as a cause of severe respiratory disease highlights the need for effective approaches to CoV vaccine development. Efforts focused solely on the receptor-binding domain (RBD) of the viral Spike (S) glycoprotein may not optimize neutralizing antibody (NAb) responses. Here we show that immunogens based on full-length S DNA and S1 subunit protein elicit robust serum-neutralizing activity against several MERS-CoV strains in mice and non-human primates. Serological analysis and isolation of murine monoclonal antibodies revealed that immunization elicits NAbs to RBD and, non-RBD portions of S1 and S2 subunit. Multiple neutralization mechanisms were demonstrated by solving the atomic structure of a NAb-RBD complex, through sequencing of neutralization escape viruses and by constructing MERS-CoV S variants for serological assays. Immunization of rhesus macaques confers protection against MERS-CoV-induced radiographic pneumonia, as assessed using computerized tomography, supporting this strategy as a promising approach for MERS-CoV vaccine development.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-06CH11357; W-31-109-ENG-38

OSTI ID:: 1214709

Journal Information:: Nature Communications, Vol. 6; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 190 works

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References (64)

Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector. Naldini, L.; Blomer, U.; Gage, F. H. Proceedings of the National Academy of Sciences, Vol. 93, Issue 21 https://doi.org/10.1073/pnas.93.21.11382	journal	October 1996
PHENIX: a comprehensive Python-based system for macromolecular structure solution. Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Apollo - University of Cambridge Repository https://doi.org/10.17863/cam.45787	text	January 2010
Reverse genetics with a full-length infectious cDNA of the Middle East respiratory syndrome coronavirus Scobey, T.; Yount, B. L.; Sims, A. C. Proceedings of the National Academy of Sciences, Vol. 110, Issue 40 https://doi.org/10.1073/pnas.1311542110	journal	September 2013
A SARS DNA vaccine induces neutralizing antibody and cellular immune responses in healthy adults in a Phase I clinical trial Martin, Julie E.; Louder, Mark K.; Holman, LaSonji A. Vaccine, Vol. 26, Issue 50 https://doi.org/10.1016/j.vaccine.2008.09.026	journal	November 2008
Purified coronavirus spike protein nanoparticles induce coronavirus neutralizing antibodies in mice Coleman, Christopher M.; Liu, Ye V.; Mu, Haiyan Vaccine, Vol. 32, Issue 26 https://doi.org/10.1016/j.vaccine.2014.04.016	journal	May 2014
Structure of RSV Fusion Glycoprotein Trimer Bound to a Prefusion-Specific Neutralizing Antibody McLellan, J. S.; Chen, M.; Leung, S. Science, Vol. 340, Issue 6136 https://doi.org/10.1126/science.1234914	journal	April 2013
Intranasal vaccination with recombinant receptor-binding domain of MERS-CoV spike protein induces much stronger local mucosal immune responses than subcutaneous immunization: Implication for designing novel mucosal MERS vaccines Ma, Cuiqing; Li, Ye; Wang, Lili Vaccine, Vol. 32, Issue 18 https://doi.org/10.1016/j.vaccine.2014.02.004	journal	April 2014
The Receptor Binding Domain of the New Middle East Respiratory Syndrome Coronavirus Maps to a 231-Residue Region in the Spike Protein That Efficiently Elicits Neutralizing Antibodies Mou, H.; Raj, V. S.; van Kuppeveld, F. J. M. Journal of Virology, Vol. 87, Issue 16 https://doi.org/10.1128/JVI.01277-13	journal	June 2013
Evasion of antibody neutralization in emerging severe acute respiratory syndrome coronaviruses Yang, Z. -y.; Werner, H. C.; Kong, W. -p. Proceedings of the National Academy of Sciences, Vol. 102, Issue 3 https://doi.org/10.1073/pnas.0409065102	journal	January 2005
Refinement of severely incomplete structures with maximum likelihood in BUSTER–TNT Blanc, E.; Roversi, P.; Vonrhein, C. Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12 https://doi.org/10.1107/S0907444904016427	journal	November 2004
Molecular basis of binding between novel human coronavirus MERS-CoV and its receptor CD26 Lu, Guangwen; Hu, Yawei; Wang, Qihui Nature, Vol. 500, Issue 7461 https://doi.org/10.1038/nature12328	journal	July 2013
Middle East respiratory syndrome coronavirus: quantification of the extent of the epidemic, surveillance biases, and transmissibility Cauchemez, Simon; Fraser, Christophe; Van Kerkhove, Maria D. The Lancet Infectious Diseases, Vol. 14, Issue 1 https://doi.org/10.1016/s1473-3099(13)70304-9	journal	January 2014
A Truncated Receptor-Binding Domain of MERS-CoV Spike Protein Potently Inhibits MERS-CoV Infection and Induces Strong Neutralizing Antibody Responses: Implication for Developing Therapeutics and Vaccines Du, Lanying; Kou, Zhihua; Ma, Cuiqing PLoS ONE, Vol. 8, Issue 12 https://doi.org/10.1371/journal.pone.0081587	journal	December 2013
A decade after SARS: strategies for controlling emerging coronaviruses Graham, Rachel L.; Donaldson, Eric F.; Baric, Ralph S. Macmillan Publishers https://doi.org/10.17615/ss8c-tg10	text	January 2013
DNA priming and influenza vaccine immunogenicity: two phase 1 open label randomised clinical trials Ledgerwood, Julie E.; Wei, Chih-Jen; Hu, Zonghui The Lancet Infectious Diseases, Vol. 11, Issue 12 https://doi.org/10.1016/S1473-3099(11)70240-7	journal	December 2011
Human Infection with MERS Coronavirus after Exposure to Infected Camels, Saudi Arabia, 2013 Memish, Ziad A.; Cotten, Matthew; Meyer, Benjamin Emerging Infectious Diseases, Vol. 20, Issue 6 https://doi.org/10.3201/eid2006.140402	journal	June 2014
Rapid generation of a mouse model for Middle East respiratory syndrome Zhao, Jincun; Li, Kun; Wohlford-Lenane, Christine Proceedings of the National Academy of Sciences, Vol. 111, Issue 13 https://doi.org/10.1073/pnas.1323279111	journal	March 2014
Structure-Based Design of a Fusion Glycoprotein Vaccine for Respiratory Syncytial Virus McLellan, J. S.; Chen, M.; Joyce, M. G. Science, Vol. 342, Issue 6158 https://doi.org/10.1126/science.1243283	journal	October 2013
Effects of Human Anti-Spike Protein Receptor Binding Domain Antibodies on Severe Acute Respiratory Syndrome Coronavirus Neutralization Escape and Fitness Marascoa, Wayne A.; Zhu, Quan Karen; Rockx, Barry American Society for Microbiology https://doi.org/10.17615/3qjm-qq07	text	January 2014
Phase I clinical evaluation of a six-plasmid multiclade HIV-1 DNA candidate vaccine Catanzaro, Andrew T.; Roederer, Mario; Koup, Richard A. Vaccine, Vol. 25, Issue 20 https://doi.org/10.1016/j.vaccine.2007.02.050	journal	May 2007
DNA Priming Prior to Inactivated Influenza A(H5N1) Vaccination Expands the Antibody Epitope Repertoire and Increases Affinity Maturation in a Boost-Interval–Dependent Manner in Adults Khurana, Surender; Wu, Jian; Dimitrova, Milena The Journal of Infectious Diseases, Vol. 208, Issue 3 https://doi.org/10.1093/infdis/jit178	journal	April 2013
Structural Insights into Immune Recognition of the Severe Acute Respiratory Syndrome Coronavirus S Protein Receptor Binding Domain Pak, John E.; Sharon, Chetna; Satkunarajah, Malathy Journal of Molecular Biology, Vol. 388, Issue 4 https://doi.org/10.1016/j.jmb.2009.03.042	journal	May 2009
Rapid generation of a mouse model for Middle East respiratory syndrome Christine, Wohlford-Lenane,; B., McCray, Paul; Jincun, Zhao, The University of North Carolina at Chapel Hill University Libraries https://doi.org/10.17615/45jv-j334	text	January 2014
Effects of Human Anti-Spike Protein Receptor Binding Domain Antibodies on Severe Acute Respiratory Syndrome Coronavirus Neutralization Escape and Fitness Sui, J.; Deming, M.; Rockx, B. Journal of Virology, Vol. 88, Issue 23 https://doi.org/10.1128/JVI.02232-14	journal	September 2014
MolProbity : all-atom structure validation for macromolecular crystallography Chen, Vincent B.; Arendall, W. Bryan; Headd, Jeffrey J. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 1 https://doi.org/10.1107/S0907444909042073	journal	December 2009
Inhibition of Middle East Respiratory Syndrome Coronavirus Infection by Anti-CD26 Monoclonal Antibody Ohnuma, K.; Haagmans, B. L.; Hatano, R. Journal of Virology, Vol. 87, Issue 24 https://doi.org/10.1128/jvi.02448-13	journal	September 2013
Sustained Peptide-Specific Gamma Interferon T-Cell Response in Rhesus Macaques Immunized with Human Immunodeficiency Virus gag DNA Vaccines Caulfield, M. J.; Wang, S.; Smith, J. G. Journal of Virology, Vol. 76, Issue 19 https://doi.org/10.1128/jvi.76.19.10038-10043.2002	journal	October 2002
Modulation of immune responses in mice to recombinant antigens from PE and PPE families of proteins of Mycobacterium tuberculosis by the Ribi adjuvant Chaitra, M. G.; Nayak, R.; Shaila, M. S. Vaccine, Vol. 25, Issue 41 https://doi.org/10.1016/j.vaccine.2007.07.026	journal	October 2007
A decade after SARS: strategies for controlling emerging coronaviruses Graham, Rachel L.; Donaldson, Eric F.; Baric, Ralph S. Nature Reviews Microbiology, Vol. 11, Issue 12 https://doi.org/10.1038/nrmicro3143	journal	November 2013
The Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Does Not Replicate in Syrian Hamsters de Wit, Emmie; Prescott, Joseph; Baseler, Laura PLoS ONE, Vol. 8, Issue 7 https://doi.org/10.1371/journal.pone.0069127	journal	July 2013
PHENIX: a comprehensive Python-based system for macromolecular structure solution Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221 https://doi.org/10.1107/S0907444909052925	journal	January 2010
DNA-Mediated Immunization in Mice Induces a Potent MHC Class I-Restricted Cytotoxic T Lymphocyte Response to the Hepatitis B Envelope Protein Davis, Heather L.; Schirmbeck, Reinhold; Reimann, Jörg Human Gene Therapy, Vol. 6, Issue 11 https://doi.org/10.1089/hum.1995.6.11-1447	journal	November 1995
Three-dimensional structure of an Fv from a human IgM immunoglobulin Fan, Zhao-chang; Shan, Lin; Guddat, Luke W. Journal of Molecular Biology, Vol. 228, Issue 1 https://doi.org/10.1016/0022-2836(92)90500-J	journal	November 1992
A DNA vaccine induces SARS coronavirus neutralization and protective immunity in mice Yang, Zhi-yong; Kong, Wing-pui; Huang, Yue Nature, Vol. 428, Issue 6982 https://doi.org/10.1038/nature02463	journal	April 2004
Middle East Respiratory Syndrome Coronavirus Quasispecies That Include Homologues of Human Isolates Revealed through Whole-Genome Analysis and Virus Cultured from Dromedary Camels in Saudi Arabia Briese, Thomas; Mishra, Nischay; Jain, Komal mBio, Vol. 5, Issue 3 https://doi.org/10.1128/mBio.01146-14	journal	April 2014
Identification of human neutralizing antibodies against MERS-CoV and their role in virus adaptive evolution A., Marasco, Wayne; Jared, Sheehan,; L., Graham, Rachel The University of North Carolina at Chapel Hill University Libraries https://doi.org/10.17615/7xm3-1892	text	January 2014
Potent cross-reactive neutralization of SARS coronavirus isolates by human monoclonal antibodies Zhu, Z.; Chakraborti, S.; He, Y. Proceedings of the National Academy of Sciences, Vol. 104, Issue 29 https://doi.org/10.1073/pnas.0701000104	journal	July 2007
[20] Processing of X-ray diffraction data collected in oscillation mode Otwinowski, Zbyszek; Minor, Wladek Macromolecular Crystallography Part A, 307-326 https://doi.org/10.1016/S0076-6879(97)76066-X	book	January 1997
A Generic Approach to Pathological Lung Segmentation Mansoor, Awais; Bagci, Ulas; Xu, Ziyue IEEE Transactions on Medical Imaging, Vol. 33, Issue 12 https://doi.org/10.1109/tmi.2014.2337057	journal	December 2014
Two is better than one Lu, Shan The Lancet Infectious Diseases, Vol. 11, Issue 12 https://doi.org/10.1016/s1473-3099(11)70256-0	journal	December 2011
Potent Neutralization of MERS-CoV by Human Neutralizing Monoclonal Antibodies to the Viral Spike Glycoprotein Jiang, L.; Wang, N.; Zuo, T. Science Translational Medicine, Vol. 6, Issue 234 https://doi.org/10.1126/scitranslmed.3008140	journal	April 2014
Exploring the gas access routes in a [NiFeSe] hydrogenase using crystals pressurized with krypton and oxygen Zacarias, Sónia; Temporão, Adriana; Carpentier, Philippe JBIC Journal of Biological Inorganic Chemistry, Vol. 25, Issue 6 https://doi.org/10.1007/s00775-020-01814-y	journal	August 2020
Identification of human neutralizing antibodies against MERS-CoV and their role in virus adaptive evolution Tang, X. -C.; Agnihothram, S. S.; Jiao, Y. Proceedings of the National Academy of Sciences, Vol. 111, Issue 19 https://doi.org/10.1073/pnas.1402074111	journal	April 2014
Regulation of antibody isotype secretion by subsets of antigen-specific helper T cells Stevens, Tracy L.; Bossie, Alexis; Sanders, Virginia M. Nature, Vol. 334, Issue 6179 https://doi.org/10.1038/334255a0	journal	July 1988
Assessing the pandemic potential of MERS-CoV Bauch, Chris T.; Oraby, Tamer The Lancet, Vol. 382, Issue 9893 https://doi.org/10.1016/s0140-6736(13)61504-4	journal	August 2013
PARP1 exhibits enhanced association and catalytic efficiency with γH2A.X-nucleosome Sharma, Deepti; De Falco, Louis; Padavattan, Sivaraman Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-13641-0	journal	December 2019
Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation Tillu, Vikas A.; Rae, James; Gao, Ya Nature Communications, Vol. 12, Issue 1 https://doi.org/10.1038/s41467-021-21035-4	journal	February 2021
Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA Wang, Daming; He, Shaogui; Wang, Xiaohui Nature Biomedical Engineering, Vol. 4, Issue 12 https://doi.org/10.1038/s41551-020-00655-z	journal	December 2020
Coronavirus IBV: Structural Characterization of the Spike Protein Cavanagh, D. Journal of General Virology, Vol. 64, Issue 12 https://doi.org/10.1099/0022-1317-64-12-2577	journal	December 1983
Features and development of Coot Emsley, P.; Lohkamp, B.; Scott, W. G. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4 https://doi.org/10.1107/S0907444910007493	journal	March 2010
A Conformation-Dependent Neutralizing Monoclonal Antibody Specifically Targeting Receptor-Binding Domain in Middle East Respiratory Syndrome Coronavirus Spike Protein Du, L.; Zhao, G.; Yang, Y. Journal of Virology, Vol. 88, Issue 12 https://doi.org/10.1128/jvi.00433-14	journal	April 2014
Is MERS another SARS? Drosten, Christian The Lancet Infectious Diseases, Vol. 13, Issue 9 https://doi.org/10.1016/s1473-3099(13)70159-2	journal	September 2013
Epidemiological, demographic, and clinical characteristics of 47 cases of Middle East respiratory syndrome coronavirus disease from Saudi Arabia: a descriptive study Assiri, Abdullah; Al-Tawfiq, Jaffar A.; Al-Rabeeah, Abdullah A. The Lancet Infectious Diseases, Vol. 13, Issue 9 https://doi.org/10.1016/S1473-3099(13)70204-4	journal	September 2013
Interhuman transmissibility of Middle East respiratory syndrome coronavirus: estimation of pandemic risk Breban, Romulus; Riou, Julien; Fontanet, Arnaud The Lancet, Vol. 382, Issue 9893 https://doi.org/10.1016/S0140-6736(13)61492-0	journal	August 2013
Contributions of the structural proteins of severe acute respiratory syndrome coronavirus to protective immunity Buchholz, U. J.; Bukreyev, A.; Yang, L. Proceedings of the National Academy of Sciences, Vol. 101, Issue 26 https://doi.org/10.1073/pnas.0403492101	journal	June 2004
Prime-Boost Interval Matters: A Randomized Phase 1 Study to Identify the Minimum Interval Necessary to Observe the H5 DNA Influenza Vaccine Priming Effect Ledgerwood, Julie E.; Zephir, Kathryn; Hu, Zonghui The Journal of Infectious Diseases, Vol. 208, Issue 3 https://doi.org/10.1093/infdis/jit180	journal	April 2013
Reverse genetics with a full-length infectious cDNA of the Middle East respiratory syndrome coronavirus Yount, Boyd L.; Menachery, Vineet D.; Graham, Rachel L. The University of North Carolina at Chapel Hill University Libraries https://doi.org/10.17615/kqa5-9507	text	January 2013
A Human T-Cell Leukemia Virus Type 1 Regulatory Element Enhances the Immunogenicity of Human Immunodeficiency Virus Type 1 DNA Vaccines in Mice and Nonhuman Primates Barouch, D. H.; Yang, Z. -y.; Kong, W. -p. Journal of Virology, Vol. 79, Issue 14 https://doi.org/10.1128/JVI.79.14.8828-8834.2005	journal	June 2005
Modulation of the Immune Response to the Severe Acute Respiratory Syndrome Spike Glycoprotein by Gene-Based and Inactivated Virus Immunization Kong, W. -p.; Xu, L.; Stadler, K. Journal of Virology, Vol. 79, Issue 22 https://doi.org/10.1128/jvi.79.22.13915-13923.2005	journal	October 2005
Structure of Severe Acute Respiratory Syndrome Coronavirus Receptor-binding Domain Complexed with Neutralizing Antibody Prabakaran, Ponraj; Gan, Jianhua; Feng, Yang Journal of Biological Chemistry, Vol. 281, Issue 23 https://doi.org/10.1074/jbc.M600697200	journal	April 2006
Inhibitory effects of aprotinin on influenza A and B viruses in vitro and in vivo Song, Eun-Jung; Españo, Erica; Shim, Sang-Mu Scientific Reports, Vol. 11, Issue 1 https://doi.org/10.1038/s41598-021-88886-1	journal	May 2021
A Simple Method of Estimating Fifty per cent Endpoints12 Reed, L. J.; Muench, H. American Journal of Epidemiology, Vol. 27, Issue 3 https://doi.org/10.1093/oxfordjournals.aje.a118408	journal	May 1938
Phaser crystallographic software McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D. Journal of Applied Crystallography, Vol. 40, Issue 4 https://doi.org/10.1107/S0021889807021206	journal	July 2007
DNA vaccination: antigen presentation and the induction of immunity Shedlock, Devon J.; Weiner, David B. Journal of Leukocyte Biology, Vol. 68, Issue 6 https://doi.org/10.1189/jlb.68.6.793	journal	December 2000

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Recent Advances in the Vaccine Development Against Middle East Respiratory Syndrome-Coronavirus Yong, Chean Yeah; Ong, Hui Kian; Yeap, Swee Keong Frontiers in Microbiology, Vol. 10 https://doi.org/10.3389/fmicb.2019.01781	journal	August 2019
Vaccines for emerging infectious diseases: Lessons from MERS coronavirus and Zika virus Maslow, Joel N. Human Vaccines & Immunotherapeutics, Vol. 13, Issue 12 https://doi.org/10.1080/21645515.2017.1358325	journal	August 2017
Comparative Serological Study for the Prevalence of Anti-MERS Coronavirus Antibodies in High- and Low-Risk Groups in Qatar Al Kahlout, Reham A.; Nasrallah, Gheyath K.; Farag, Elmoubasher A. Journal of Immunology Research, Vol. 2019 https://doi.org/10.1155/2019/1386740	journal	February 2019
Junctional and allele-specific residues are critical for MERS-CoV neutralization by an exceptionally potent germline-like antibody Ying, Tianlei; Prabakaran, Ponraj; Du, Lanying Nature Communications, Vol. 6, Issue 1 https://doi.org/10.1038/ncomms9223	journal	September 2015
Immunogenicity of Candidate MERS-CoV DNA Vaccines Based on the Spike Protein Al-amri, Sawsan S.; Abbas, Ayman T.; Siddiq, Loai A. Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/srep44875	journal	March 2017
MERS-CoV spike protein: a key target for antivirals Du, Lanying; Yang, Yang; Zhou, Yusen Expert Opinion on Therapeutic Targets, Vol. 21, Issue 2 https://doi.org/10.1080/14728222.2017.1271415	journal	December 2016
Epitope‐based peptide vaccine design and target site depiction against Middle East Respiratory Syndrome Coronavirus: an immune-informatics study Tahir ul Qamar, Muhammad; Saleem, Saman; Ashfaq, Usman Ali Journal of Translational Medicine, Vol. 17, Issue 1 https://doi.org/10.1186/s12967-019-2116-8	journal	November 2019
ChAdOx1 and MVA based vaccine candidates against MERS-CoV elicit neutralising antibodies and cellular immune responses in mice Alharbi, Naif Khalaf; Padron-Regalado, Eriko; Thompson, Craig P. Vaccine, Vol. 35, Issue 30 https://doi.org/10.1016/j.vaccine.2017.05.032	journal	June 2017
DNA vaccine encoding Middle East respiratory syndrome coronavirus S1 protein induces protective immune responses in mice Chi, Hang; Zheng, Xuexing; Wang, Xiwen Vaccine, Vol. 35, Issue 16 https://doi.org/10.1016/j.vaccine.2017.02.063	journal	April 2017
Live-attenuated bivalent measles virus-derived vaccines targeting Middle East respiratory syndrome coronavirus induce robust and multifunctional T cell responses against both viruses in an appropriate mouse model Bodmer, Bianca S.; Fiedler, Anna H.; Hanauer, Jan R. H. Virology, Vol. 521 https://doi.org/10.1016/j.virol.2018.05.028	journal	August 2018
CD8+ T Cells Responding to the Middle East Respiratory Syndrome Coronavirus Nucleocapsid Protein Delivered by Vaccinia Virus MVA in Mice Veit, Svenja; Jany, Sylvia; Fux, Robert Viruses, Vol. 10, Issue 12 https://doi.org/10.3390/v10120718	journal	December 2018
A Highly Immunogenic, Protective, and Safe Adenovirus-Based Vaccine Expressing Middle East Respiratory Syndrome Coronavirus S1-CD40L Fusion Protein in a Transgenic Human Dipeptidyl Peptidase 4 Mouse Model Hashem, Anwar M.; Algaissi, Abdullah; Agrawal, Anurodh Shankar The Journal of Infectious Diseases, Vol. 220, Issue 10 https://doi.org/10.1093/infdis/jiz137	journal	July 2019
Adjuvants and the vaccine response to the DS-Cav1-stabilized fusion glycoprotein of respiratory syncytial virus Sastry, Mallika; Zhang, Baoshan; Chen, Man PLOS ONE, Vol. 12, Issue 10 https://doi.org/10.1371/journal.pone.0186854	journal	October 2017
Heterologous prime–boost vaccination with adenoviral vector and protein nanoparticles induces both Th1 and Th2 responses against Middle East respiratory syndrome coronavirus Jung, Seo-Yeon; Kang, Kyung Won; Lee, Eun-Young Vaccine, Vol. 36, Issue 24 https://doi.org/10.1016/j.vaccine.2018.04.082	journal	June 2018
Strategies Using Bio-Layer Interferometry Biosensor Technology for Vaccine Research and Development Petersen, Rejane Biosensors, Vol. 7, Issue 4 https://doi.org/10.3390/bios7040049	journal	October 2017
Production of a Monoclonal Antibody Targeting the M Protein of MERS-CoV for Detection of MERS-CoV Using a Synthetic Peptide Epitope Formulated with a CpG–DNA–Liposome Complex Park, Byoung Kwon; Lee, Su In; Bae, Joon-Yong International Journal of Peptide Research and Therapeutics, Vol. 25, Issue 3 https://doi.org/10.1007/s10989-018-9731-8	journal	July 2018
Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigen Pallesen, Jesper; Wang, Nianshuang; Corbett, Kizzmekia S. Proceedings of the National Academy of Sciences, Vol. 114, Issue 35 https://doi.org/10.1073/pnas.1707304114	journal	August 2017
Subunit Vaccines Against Emerging Pathogenic Human Coronaviruses Wang, Ning; Shang, Jian; Jiang, Shibo Frontiers in Microbiology, Vol. 11 https://doi.org/10.3389/fmicb.2020.00298	journal	February 2020
Middle East respiratory syndrome: current status and future prospects for vaccine development Du, Lanying; Jiang, Shibo Expert Opinion on Biological Therapy, Vol. 15, Issue 11 https://doi.org/10.1517/14712598.2015.1092518	journal	July 2015
Antibodies and vaccines against Middle East respiratory syndrome coronavirus Xu, Jiuyang; Jia, Wenxu; Wang, Pengfei Emerging Microbes & Infections, Vol. 8, Issue 1 https://doi.org/10.1080/22221751.2019.1624482	journal	January 2019
Vaccines for the prevention against the threat of MERS-CoV Du, Lanying; Tai, Wanbo; Zhou, Yusen Expert Review of Vaccines, Vol. 15, Issue 9 https://doi.org/10.1586/14760584.2016.1167603	journal	April 2016
Enhanced protection in mice induced by immunization with inactivated whole viruses compare to spike protein of middle east respiratory syndrome coronavirus Deng, Yao; Lan, Jiaming; Bao, Linlin Emerging Microbes & Infections, Vol. 7, Issue 1 https://doi.org/10.1038/s41426-018-0056-7	journal	April 2018
Cryo-EM structures of MERS-CoV and SARS-CoV spike glycoproteins reveal the dynamic receptor binding domains Yuan, Yuan; Cao, Duanfang; Zhang, Yanfang Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/ncomms15092	journal	April 2017
Ultrapotent Human Neutralizing Antibody Repertoires Against Middle East Respiratory Syndrome Coronavirus From a Recovered Patient Niu, Peihua; Zhang, Senyan; Zhou, Panpan The Journal of Infectious Diseases, Vol. 218, Issue 8 https://doi.org/10.1093/infdis/jiy311	journal	May 2018
A recombinant VSV-vectored MERS-CoV vaccine induces neutralizing antibody and T cell responses in rhesus monkeys after single dose immunization Liu, Renqiang; Wang, Jinliang; Shao, Yu Antiviral Research, Vol. 150 https://doi.org/10.1016/j.antiviral.2017.12.007	journal	February 2018
A high-throughput inhibition assay to study MERS-CoV antibody interactions using image cytometry Rosen, Osnat; Chan, Leo Li-Ying; Abiona, Olubukola M. Journal of Virological Methods, Vol. 265 https://doi.org/10.1016/j.jviromet.2018.11.009	journal	March 2019
Narrative review of Middle East respiratory syndrome coronavirus (MERS-CoV) infection: updates and implications for practice Al Mutair, Abbas; Ambani, Zainab Journal of International Medical Research https://doi.org/10.1177/0300060519858030	journal	July 2019
Structural basis of development of multi-epitope vaccine against Middle East respiratory syndrome using in silico approach Srivastava, Sukrit; Kamthania, Mohit; Singh, Soni Infection and Drug Resistance, Vol. Volume 11 https://doi.org/10.2147/idr.s175114	journal	November 2018
Viromimetic STING Agonist‐Loaded Hollow Polymeric Nanoparticles for Safe and Effective Vaccination against Middle East Respiratory Syndrome Coronavirus Lin, Leon Chien‐Wei; Huang, Chen‐Yu; Yao, Bing‐Yu Advanced Functional Materials, Vol. 29, Issue 28 https://doi.org/10.1002/adfm.201807616	journal	April 2019

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