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Title: Insights into the respiratory tract microbiota of patients with cystic fibrosis during early Pseudomonas aeruginosa colonization

Abstract

Pseudomonas aeruginosa plays a major role in cystic fibrosis (CF) progression. Therefore, it is important to understand the initial steps of P. aeruginosa infection. The structure and dynamics of CF respiratory tract microbial communities during the early stages of P. aeruginosa colonization were characterized by pyrosequencing and cloning-sequencing. The respiratory microbiota showed high diversity, related to the young age of the CF cohort (mean age 10 years). Wide inter- and intra-individual variations were revealed. A common core microbiota of 5 phyla and 13 predominant genera was found, the majority of which were obligate anaerobes. A few genera were significantly more prevalent in patients never infected by P. aeruginosa. Persistence of an anaerobic core microbiota regardless of P. aeruginosa status suggests a major role of certain anaerobes in the pathophysiology of lung infections in CF. Some genera may be potential biomarkers of pulmonary infection state.

Authors:
 [1];  [1];  [2];  [3];  [4];  [1];  [5];  [1];  [6];  [1];  [1];  [3]
  1. Univ. of Brest, Brest Cedex (France)
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Research and Development Luminy Biotech Enterprises, Marseille (France)
  3. Univ. of Brest, Brest Cedex (France); Univ. Hospital of Brest, Brest (France)
  4. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  5. Univ. Hospital of Brest, Brest (France)
  6. CF Center, Roscoff (France)
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE; French Cystic Fibrosis Association: 'Vaincre la Mucoviscidose'; French Ministry of Higher Education and Research (Ministère de l’Enseignement Supérieur et de la Recherche: MESR, France)
OSTI Identifier:
1212084
Grant/Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
SpringerPlus
Additional Journal Information:
Journal Volume: 4; Journal Issue: 1; Journal ID: ISSN 2193-1801
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; cystic fibrosis; respiratory tract microbiota; Pseudomonas aeruginosa; early colonization; anaerobes; respiratory viruses

Citation Formats

Keravec, Marlène, Mounier, Jérôme, Prestat, Emmanuel, Vallet, Sophie, Jansson, Janet K., Burgaud, Gaëtan, Rosec, Sylvain, Gouriou, Stéphanie, Rault, Gilles, Coton, Emmanuel, Barbier, Georges, and Héry-Arnaud, Geneviève. Insights into the respiratory tract microbiota of patients with cystic fibrosis during early Pseudomonas aeruginosa colonization. United States: N. p., 2015. Web. doi:10.1186/s40064-015-1207-0.
Keravec, Marlène, Mounier, Jérôme, Prestat, Emmanuel, Vallet, Sophie, Jansson, Janet K., Burgaud, Gaëtan, Rosec, Sylvain, Gouriou, Stéphanie, Rault, Gilles, Coton, Emmanuel, Barbier, Georges, & Héry-Arnaud, Geneviève. Insights into the respiratory tract microbiota of patients with cystic fibrosis during early Pseudomonas aeruginosa colonization. United States. https://doi.org/10.1186/s40064-015-1207-0
Keravec, Marlène, Mounier, Jérôme, Prestat, Emmanuel, Vallet, Sophie, Jansson, Janet K., Burgaud, Gaëtan, Rosec, Sylvain, Gouriou, Stéphanie, Rault, Gilles, Coton, Emmanuel, Barbier, Georges, and Héry-Arnaud, Geneviève. 2015. "Insights into the respiratory tract microbiota of patients with cystic fibrosis during early Pseudomonas aeruginosa colonization". United States. https://doi.org/10.1186/s40064-015-1207-0. https://www.osti.gov/servlets/purl/1212084.
@article{osti_1212084,
title = {Insights into the respiratory tract microbiota of patients with cystic fibrosis during early Pseudomonas aeruginosa colonization},
author = {Keravec, Marlène and Mounier, Jérôme and Prestat, Emmanuel and Vallet, Sophie and Jansson, Janet K. and Burgaud, Gaëtan and Rosec, Sylvain and Gouriou, Stéphanie and Rault, Gilles and Coton, Emmanuel and Barbier, Georges and Héry-Arnaud, Geneviève},
abstractNote = {Pseudomonas aeruginosa plays a major role in cystic fibrosis (CF) progression. Therefore, it is important to understand the initial steps of P. aeruginosa infection. The structure and dynamics of CF respiratory tract microbial communities during the early stages of P. aeruginosa colonization were characterized by pyrosequencing and cloning-sequencing. The respiratory microbiota showed high diversity, related to the young age of the CF cohort (mean age 10 years). Wide inter- and intra-individual variations were revealed. A common core microbiota of 5 phyla and 13 predominant genera was found, the majority of which were obligate anaerobes. A few genera were significantly more prevalent in patients never infected by P. aeruginosa. Persistence of an anaerobic core microbiota regardless of P. aeruginosa status suggests a major role of certain anaerobes in the pathophysiology of lung infections in CF. Some genera may be potential biomarkers of pulmonary infection state.},
doi = {10.1186/s40064-015-1207-0},
url = {https://www.osti.gov/biblio/1212084}, journal = {SpringerPlus},
issn = {2193-1801},
number = 1,
volume = 4,
place = {United States},
year = {Sun Aug 09 00:00:00 EDT 2015},
month = {Sun Aug 09 00:00:00 EDT 2015}
}

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Cited by: 22 works
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Works referenced in this record:

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Sputum Microbiota in Tuberculosis as Revealed by 16S rRNA Pyrosequencing
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Airway Microbiota and Pathogen Abundance in Age-Stratified Cystic Fibrosis Patients
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UCHIME improves sensitivity and speed of chimera detection
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Airway microbiota and bronchial hyperresponsiveness in patients with suboptimally controlled asthma
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Clinical outcome after early Pseudomonas aeruginosa infection in cystic fibrosis
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Discerning the Complexity of Community Interactions Using a Drosophila Model of Polymicrobial Infections
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Investigating Deep Phylogenetic Relationships among Cyanobacteria and Plastids by Small Subunit rRNA Sequence Analysis
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Prevention of chronic Pseudomonas aeruginosa colonisation in cystic fibrosis by early treatment
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Partitioning core and satellite taxa from within cystic fibrosis lung bacterial communities
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Application of a Neutral Community Model To Assess Structuring of the Human Lung Microbiome
journal, January 2015


The role of respiratory viruses in cystic fibrosis
journal, July 2008


Spatial distribution of microbial communities in the cystic fibrosis lung
journal, July 2011


Inflammation and Airway Microbiota during Cystic Fibrosis Pulmonary Exacerbations
journal, April 2013


Detection of anaerobic bacteria in high numbers in sputum from patients with cystic fibrosis
text, January 2008


Acceleration of lung disease in children with cystic fibrosis after Pseudomonas aeruginosa acquisition
journal, October 2001


Prevention of chronic Pseudomonas aeruginosa colonisation in cystic fibrosis by early treatment
journal, September 1991


Assessment of the microbial diversity at the surface of Livarot cheese using culture-dependent and independent approaches
journal, July 2009


Airway microbiota and bronchial hyperresponsiveness in patients with suboptimally controlled asthma
journal, February 2011


Role of respiratory viruses in pulmonary exacerbations in children with cystic fibrosis
journal, September 2012


Evaluation of a new definition for chronic Pseudomonas aeruginosa infection in cystic fibrosis patients
journal, March 2003


Partitioning core and satellite taxa from within cystic fibrosis lung bacterial communities
journal, December 2010


Spatial distribution of microbial communities in the cystic fibrosis lung
journal, July 2011


Sequencing ancient calcified dental plaque shows changes in oral microbiota with dietary shifts of the Neolithic and Industrial revolutions
journal, February 2013


UPARSE: highly accurate OTU sequences from microbial amplicon reads
journal, August 2013


Clinical outcome after early Pseudomonas aeruginosa infection in cystic fibrosis
journal, May 2001


UCHIME improves sensitivity and speed of chimera detection
journal, June 2011


Investigating Deep Phylogenetic Relationships among Cyanobacteria and Plastids by Small Subunit rRNA Sequence Analysis
journal, July 1999


Quantitative Analysis of the Human Airway Microbial Ecology Reveals a Pervasive Signature for Cystic Fibrosis
journal, September 2012


Anaerobic Bacterial Infection in Patients with Cystic Fibrosis
journal, November 2008


Detection of Anaerobic Bacteria in High Numbers in Sputum from Patients with Cystic Fibrosis
journal, May 2008


Proposal of a quantitative PCR-based protocol for an optimal Pseudomonas aeruginosa detection in patients with cystic fibrosis
journal, January 2013


Airway Microbiota and Pathogen Abundance in Age-Stratified Cystic Fibrosis Patients
journal, June 2010


Sputum Microbiota in Tuberculosis as Revealed by 16S rRNA Pyrosequencing
journal, January 2013


Inflammation and Airway Microbiota during Cystic Fibrosis Pulmonary Exacerbations
journal, April 2013


Discerning the Complexity of Community Interactions Using a Drosophila Model of Polymicrobial Infections
journal, October 2008


Cystic fibrosis: a polymicrobial infectious disease
journal, June 2006


Works referencing / citing this record:

The airway microbiota in early cystic fibrosis lung disease
journal, August 2017


Inhibitory role of acyl homoserine lactones in hemolytic activity and viability of Streptococcus pyogenes M6 S165
journal, March 2017


Viruses in cystic fibrosis patients’ airways
journal, March 2017


Anaerobes in cystic fibrosis patients’ airways
journal, January 2019


The diagnosis and management of respiratory viral infections in cystic fibrosis
journal, February 2017


Cystic Fibrosis Airway Microbiome: Overturning the Old, Opening the Way for the New
journal, October 2017


Cystic fibrosis lung environment and Pseudomonas aeruginosa infection
journal, December 2016


Strong incidence of Pseudomonas aeruginosa on bacterial rrs and ITS genetic structures of cystic fibrosis sputa
journal, March 2017


Strong incidence of Pseudomonas aeruginosa on bacterial rrs and ITS genetic structures of cystic fibrosis sputa
journal, March 2017