Capture and X-ray diffraction studies of protein microcrystals in a microfluidic trap array

Lyubimov, Artem Y.; Murray, Thomas D.; Koehl, Antoine; Araci, Ismail Emre; Uervirojnangkoorn, Monarin; Zeldin, Oliver B.; Cohen, Aina E.; Soltis, S. Michael; Baxter, Elizabeth L.; Brewster, Aaron S.; Sauter, Nicholas K.; Brunger, Axel T.; Berger, James M.

doi:10.1107/S1399004715002308

Title: Capture and X-ray diffraction studies of protein microcrystals in a microfluidic trap array

Journal Article · Fri Mar 27 00:00:00 EDT 2015 · Acta Crystallographica. Section D: Biological Crystallography (Online)

DOI:https://doi.org/10.1107/S1399004715002308· OSTI ID:1189952

Lyubimov, Artem Y. ^[1]; Murray, Thomas D. ^[2]; Koehl, Antoine ^[1]; Araci, Ismail Emre ^[1]; Uervirojnangkoorn, Monarin ^[1]; Zeldin, Oliver B. ^[1]; Cohen, Aina E. ^[3]; Soltis, S. Michael ^[3]; Baxter, Elizabeth L. ^[3]; Brewster, Aaron S. ^[4]; Sauter, Nicholas K. ^[4]; Brunger, Axel T. ^[1]; Berger, James M. ^[5]

Stanford Univ., Stanford, CA (United States)
Univ. of California, Berkeley, CA (United States); Johns Hopkins Univ. School of Medicine, Balitmore, MD (United States)
SLAC National Accelerator Laboratory, Stanford, CA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Johns Hopkins Univ., Baltimore, MD (United States)

X-ray free-electron lasers (XFELs) promise to enable the collection of interpretable diffraction data from samples that are refractory to data collection at synchrotron sources. At present, however, more efficient sample-delivery methods that minimize the consumption of microcrystalline material are needed to allow the application of XFEL sources to a wide range of challenging structural targets of biological importance. Here, a microfluidic chip is presented in which microcrystals can be captured at fixed, addressable points in a trap array from a small volume (<10 µl) of a pre-existing slurry grown off-chip. The device can be mounted on a standard goniostat for conducting diffraction experiments at room temperature without the need for flash-cooling. Proof-of-principle tests with a model system (hen egg-white lysozyme) demonstrated the high efficiency of the microfluidic approach for crystal harvesting, permitting the collection of sufficient data from only 265 single-crystal still images to permit determination and refinement of the structure of the protein. This work shows that microfluidic capture devices can be readily used to facilitate data collection from protein microcrystals grown in traditional laboratory formats, enabling analysis when cryopreservation is problematic or when only small numbers of crystals are available. Such microfluidic capture devices may also be useful for data collection at synchrotron sources.

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Research Organization:: SLAC National Accelerator Lab., Menlo Park, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-76SF00515; AC02-05CH11231

OSTI ID:: 1189952

Alternate ID(s):: OSTI ID: 1512102

Journal Information:: Acta Crystallographica. Section D: Biological Crystallography (Online), Vol. 71, Issue 4; ISSN 1399-0047

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 52 works

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